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Developmental potential of somatic and germ cells of hybrids between Carassius auratus females and Hemigrammocypris rasborella males

Published online by Cambridge University Press:  10 August 2020

Yuki Naya ,
Tomoka Matsunaga ,
Yu Shimizu ,
Eisuke Takahashi ,
Fumika Shima ,
Mitsuru Endoh ,
Takafumi Fujimoto Open the ORCID record for Takafumi Fujimoto [Opens in a new window] ,
Katsutoshi Arai  and
Etsuro Yamaha Open the ORCID record for Etsuro Yamaha [Opens in a new window]
Yuki Naya
Affiliation:
Nanae Fresh-Water Station, Field Science Center for Northern Biosphere, Hokkaido University Laboratory of Aquaculture Genetics and Genomics, Faculty of Fisheries Sciences, Hokkaido University
Tomoka Matsunaga
Affiliation:
Nanae Fresh-Water Station, Field Science Center for Northern Biosphere, Hokkaido University
Yu Shimizu
Affiliation:
Nanae Fresh-Water Station, Field Science Center for Northern Biosphere, Hokkaido University Laboratory of Aquaculture Genetics and Genomics, Faculty of Fisheries Sciences, Hokkaido University
Eisuke Takahashi
Affiliation:
Nanae Fresh-Water Station, Field Science Center for Northern Biosphere, Hokkaido University
Fumika Shima
Affiliation:
Laboratory of Aquaculture Genetics and Genomics, Faculty of Fisheries Sciences, Hokkaido University
Mitsuru Endoh
Affiliation:
Laboratory of Aquaculture Genetics and Genomics, Faculty of Fisheries Sciences, Hokkaido University
Takafumi Fujimoto
Affiliation:
Laboratory of Aquaculture Genetics and Genomics, Faculty of Fisheries Sciences, Hokkaido University
Katsutoshi Arai
Affiliation:
Institute for the Advancement of Higher Education, Hokkaido University
Etsuro Yamaha*
Affiliation:
Nanae Fresh-Water Station, Field Science Center for Northern Biosphere, Hokkaido University
*
Author for correspondence: Etsuro Yamaha. 2-9-1, Sakura, Nanae, Kameda, Hokkaido, 041-1105, Japan. Tel: +81 138-65-2344. Fax: +81 138-65-2239. E-mail: eyamaha@fsc.hokudai.ac.jp
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Summary

The cause of hybrid sterility and inviability has not been analyzed in the fin-fish hybrid, although large numbers of hybridizations have been carried out. In this study, we produced allo-diploid hybrids by cross-fertilization between female goldfish (Carassius auratus) and male golden venus chub (Hemigrammocypris rasborella). Inviability of these hybrids was due to breakage of the enveloping layer during epiboly or due to malformation with serious cardiac oedema around the hatching stage. Spontaneous allo-triploid hybrids with two sets of the goldfish genome and one set of the golden venus chub genome developed normally and survived beyond the feeding stage. This improved survival was confirmed by generating heat-shock-induced allo-triploid hybrids that possessed an extra goldfish genome. When inviable allo-diploid hybrid cells were transplanted into goldfish host embryos at the blastula stage, these embryos hatched normally, incorporating the allo-diploid cells. These allo-diploid hybrid cells persisted, and were genetically detected in a 6-month-old fish. In contrast, primordial germ cells taken from allo-diploid hybrids and transplanted into goldfish hosts at the blastula stage had disappeared by 10 days post-fertilization, even under chimeric conditions. In allo-triploid hybrid embryos, germ cells proliferated in the gonad, but had disappeared by 10 weeks post-fertilization. These results showed that while hybrid germ cells are inviable even in chimeric conditions, hybrid somatic cells remain viable.

Keywords

ChimeraFertilityHybridPolyploidSterility
Type
Research Article
Information
Zygote , Volume 28 , Issue 6 , December 2020 , pp. 470 - 481
Copyright
© The Author(s), 2020. Published by Cambridge University Press

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