Abstract
Telomerase is a specialized enzyme that attributes to maintaining and lengthening of telomeric length as its primary function. This ribonucleoprotein complex requires two minimum subunits for its in vivo function: the TERT amino acid subunit and TER its ribonucleic subunit. Crystallographic studies have shown that stable interaction between subunits and their domains is essential during the active phase. Plants and humans have shown to be tissue specific and dependent on cell proliferation. In Arabidopsis thaliana (L.) Heynh, the expression of AtTERT was determined to be necessary for activity. The objective of this work is to perform the in silico characterization of TERT in Agave tequilana F.A.C. Weber and evaluate the expression of TERT and the specific tissue activity in 2-year-old plants. To this end, we use the transcriptome library of A. tequilana (Ávila de Dios unpublished data) in TPM and qTRAP. We observe in this work that the consensus sequence shows all the specific domains of the TERT subunit. Additionally, we carried out the in silico prediction of the structural model of AteqTERT. The model presents the characteristic TERT ring of the crystallized models in active state of telomerase, biological annotations were observed, such as heavy metals Mg2+, NUC and CH1, and finally we note that, there is no correlation between TERT expression in TPM and telomerase activity in 2-year meristematic tissue.
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Acknowledgements
To E. a person essential for my personal and academic development and giving me experimental guidance and knowage on this topic, thanks for sharing your experience and time with me, this project was funded by CONACyT Project CB-2012-180757-Z and by the grant awarded and Goty Bautelpacher, owner of the hacienda “MAYAPAN” farm for the plant material and June - Simpson for access to the RNA - Seq library, for its support and academic time granted.
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De la Torre Espinosa, Z., De Dios, E.Á., Sánchez Teyer, F. et al. AteqTERT expression and specific tissue activity in a 2-year-old complete plant in Agave tequilana in field conditions. Braz. J. Bot 43, 869–881 (2020). https://doi.org/10.1007/s40415-020-00642-3
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DOI: https://doi.org/10.1007/s40415-020-00642-3