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COX10-AS1 Facilitates Cell Proliferation and Inhibits Cell Apoptosis in Glioblastoma Cells at Post-Transcription Level

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Abstract

Glioblastoma (GBM) is an invasive cancer with poor prognosis in patients. Researching on molecular functions in GBM has attracted more and more attention. Actin gamma 1 (ACTG1) was reported as a pathogenic gene in skin cancer and colorectal cancer. Present study was designed to explore the biological role and underlying mechanism of ACTG1 in GBM cells. It was uncovered that ACTG1 presented high expression trends in GBM cells. Moreover, ACTG1 suppression hindered cell proliferation and boosted cell apoptosis in GBM. Then, according to the results of bioinformatics analysis and mechanism assays including RIP, RNA pull down and luciferase reporter assay, ACTG1 was verified to be targeted by miR-361-5p in GBM. Next, COX10-AS1 (COX10 antisense RNA 1) was identified as an endogenous sponge for miR-361-5p in GBM. Moreover, COX10-AS1 acted as a competing endogenous RNA (ceRNA) to positively regulate ACTG1 expression via sponging miR-361-5p. The following rescue assays demonstrated that COX10-AS1 promoted GBM cell proliferation and inhibited GBM cell apoptosis through ACTG1 up-regulation at a miR-361-5p dependent way. On the whole, present study uncovered a novel ceRNA pattern in which COX10-AS1 sponged miR-361-5p to elevate ACTG1 expression, therefore accelerating tumorigenesis in GBM. The findings suggested new promising targets for GBM treatment.

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Abbreviations

GBM:

Glioblastoma

LncRNA:

Long non-coding RNA

COX10-AS1:

COX10 antisense RNA 1

CeRNA:

Competing endogenous RNA

MiRNA:

MicroRNA

ACTG1:

Actin gamma 1

QRT-PCR:

Quantitative Real-time PCR

TUNEL:

Terminal deoxynucleotidyl transferase dUTP nick end labeling

FISH:

Fluorescence in Situ Hybridization

RIP:

RNA Binding Protein Immunoprecipitation

ShRNA:

Short hairpin RNA

FBS:

Fetal bovine serum

ATCC:

American Type Culture Collection

DMEM:

Dulbecco’s modified Eagle’s medium

RISC:

RNA induced silence complex

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We sincerely appreciate all lab members.

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Authors and Affiliations

  1. Department of Neurosurgery, Jiangxi Provincial People’s Hospital Affiliated to Nanchang University, No.92 Aiguo Road, Nanchang, 330006, Jiangxi, China

    Chaoyang Zhou, Xingxing Jiang, Aijun Liang, Ronglan Zhu, Yu Yang, Liangchen Zhong & Dengfeng Wan

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  1. Chaoyang Zhou
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  2. Xingxing Jiang
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  3. Aijun Liang
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  4. Ronglan Zhu
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  5. Yu Yang
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  6. Liangchen Zhong
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Correspondence to Dengfeng Wan.

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Zhou, C., Jiang, X., Liang, A. et al. COX10-AS1 Facilitates Cell Proliferation and Inhibits Cell Apoptosis in Glioblastoma Cells at Post-Transcription Level. Neurochem Res 45, 2196–2203 (2020). https://doi.org/10.1007/s11064-020-03081-4

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  • DOI: https://doi.org/10.1007/s11064-020-03081-4

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