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Cloning and tissue distribution of the ATP-binding cassette subfamily G member 2 gene in the marine pufferfish Takifugu rubripes

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  • Chemistry and Biochemistry
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Abstract

In this study, we cloned the ATP-binding cassette (ABC) subfamily G member 2 gene (Abcg2 gene; breast cancer resistance protein) from the liver of the marine pufferfish Takifugu rubripes, and examined its tissue distribution and gene structure. The full-length complementary DNA (cDNA) was 1977 base pairs (bp), comprising 15 exons in chromosome 17, and was assigned to the teleost Abcg2b paralogue. The open reading frame was 1839 bp in length, and encodes 612 amino acids constituting Walker A, Q-loop, C motif, Walker B, D-loop, and H-loop sequences in the first half and six transmembrane domains in the second half. Gene expression of Abcg2 was predominantly found in the intestines, followed by the liver and kidneys. The Kozak motif and the polyadenylation signal sequences were at respective ends of the transcript. The putative core promoter sequences including the transcription factor II B recognition elements, the TATA box, the initiator element, and the downstream core promoter element were found in the transcript and its upstream genomic DNA sequence. The hypoxia response element and the estrogen response element were located in the upstream genomic DNA sequence, suggesting that the pufferfish Abcg2 gene possesses the same regulation mechanism of transcription as the human ABCG2 gene.

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Acknowledgments

This work was partly supported by an academic grant from the Toyo Suisan Foundation, and a Grant-in-Aid for Scientific Research (B) (15H04550) from the Ministry of Education, Culture, Sports, Science and Technology, Japan.

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Authors and Affiliations

  1. Department of Health Sciences, Faculty of Human Culture and Science, Prefectural University of Hiroshima, Ujina-higashi 1-1-71, Minami-ku, Hiroshima, 734-8558, Japan

    Takuya Matsumoto

  2. Department of Environmental Sciences, Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, Nanatsuka 5562, Shobara, Hiroshima, 727-0023, Japan

    Saemi Kitajima, Chisato Yamamoto, Mitsuru Aoyagi, Yoshiharu Mitoma & Hiroyuki Harada

  3. Faculty of Agro-Food Science, Niigata Agro-Food University, Hiranedai 2416, Tainai, Niigata, 959-2702, Japan

    Yuji Nagashima

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  1. Takuya Matsumoto
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  4. Mitsuru Aoyagi
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Correspondence to Takuya Matsumoto.

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Matsumoto, T., Kitajima, S., Yamamoto, C. et al. Cloning and tissue distribution of the ATP-binding cassette subfamily G member 2 gene in the marine pufferfish Takifugu rubripes. Fish Sci 86, 873–887 (2020). https://doi.org/10.1007/s12562-020-01451-z

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