Abstract
A field-liberation technique for cocoons of the parasitoid Cotesia flavipes Cameron (Hymenoptera: Braconidae) against sugarcane borers was developed in two stages: (i) laboratory standardization of a gravimetric method to enumerate cocoons for field release and (ii) design and evaluation of a field-release device to dispense cocoons of the parasitoid. Field-collected and laboratory-reared cocoon masses of C. flavipes from two larval hosts were characterized for cocoon mass weight, cocoon number per mass (cocoon mass size), percent of adult emergence and percent of females. Non-parametric ANOVA of cocoon characteristics showed no significant differences among field-collected and laboratory-reared larvae of the two species. Cocoon mass size vs. cocoon mass weight correlation was significant for field-collected and laboratory-reared larvae of both species. Coincidence analysis of the four regression lines indicated adequacy of a single line to describe cocoon mass size vs. cocoon mass weight relationship and predict the former from the latter. Laboratory and field tests with a prototype field-release device established egress of the parasitoid from the device with minimum risk of predation. The protocol developed for dispensing C. flavipes cocoons in the field includes three steps: (i) determination of gross dosage from net dosage using proportions of females and adult emergence as correction factors; (ii) estimation of cocoon number in individual cocoon masses from cocoon mass weight using the regression equation and (iii) dispensation of gross dosage of cocoon masses using the field-release device.
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References
Anonymous. (2009). SBI Annual Report 2008–09 (82p). Coimbatore: ICAR-Sugarcane Breeding Institute.
Anonymous. (2010). SBI Annual Report 2009–10 (110p). Coimbatore: ICAR-Sugarcane Breeding Institute.
Anonymous. (2018). SBI annual report 2017–18 (208p). Coimbatore: ICAR-Sugarcane Breeding Institute.
Arakaki, N., & Ganaha, Y. (1986). Emergence pattern and mating behaviour of Apanteles flavipes (Cameron) (Hymenoptera: Braconidae). Applied Entomology and Zoology, 21(3), 382–388.
Cramer, D., & Howitt, D. L. (2004). The SAGE dictionary of statistics: A practical resource for students in social sciences. London: SAGE Publications Ltd..
Easwaramoorthy, S., & Shanmugasundaram, M. (1991). Mass rearing of Sesamia inferens Wlk. and Chilo sacchariphagus indicus (Kapur). In H. David & S. Easwaramoorthy (Eds.), Biocontrol Technology for Sugarcane Pest Management (pp. 101–108). Coimbatore: ICAR-Sugarcane Breeding Institute.
Easwaramoorthy, S., Srikanth, J., Shanmugasundaram, M., & Kumar, R. (1998). Field evaluation of Cotesia flavipes against internode borer Chilo sacchariphagus indicus of sugarcane. Sugarcane, 2, 18–21.
Eickwort, G. C. (1981). Presocial insects. In H. R. Hermann (Ed.), Social insects (Vol. II, pp. 199–280). New York: Academic Press.
Gupta, B. D., & Avasthy, P. N. (1959). Biology and control of the stem borer, Chilo tumidicostalis Hmpsn. Proceedings of the Congress, International Society of Sugarcane Technologists, 10, 886–901.
Hailemichael, Y., Schulthess, F., Smith Jr., J. W., & Overholt, W. A. (2009). Physiological suitability of six west African gramineous borers (Lepidoptera: Noctuidae, Pyralidae) for development of Cotesia species complex (Hymenoptera: Braconidae). International Journal of Tropical Insect Science, 29(3), 130–140.
Kajitha, H., & Drake, E. F. (1969). Biology of Apanteles chilonis and A. flavipes (Hymenoptera: Braconidae), parasites of Chilo suppressalis. Mushi, 42, 163–179.
Mitchell, E. R., Tingle, F. C., Navasero-Ward, R. C., & Kehat, M. (1997). Diamondback moth (Lepidoptera: Plutellidae): Parasitism by Cotesia plutellae (Hymenoptera: Braconidae) in cabbage. Florida Entomologist, 80(4), 477–489.
Omwega, C. O., Muchugu, E., Overholt, W. A., & Schulthess, F. (2006). Release and establishment of Cotesia flavipes Cameron (Hymenoptera: Braconidae) an exotic parasitoid of Chilo partellus (Swinhoe) (Lepidoptera: Crambidae) in east and southern Africa. Annales de la Société Entomologique de France, 42(3–4), 511–517.
Overholt, W. A., Ochieng, J. O., Lammers, P., & Ogedah, K. (1994). Rearing and field release methods for Cotesia flavipes Cameron (Hymenoptera: Braconidae), a parasitoid of tropical gramineous stem borers. Insect Science and its Application, 15(3), 253–259.
Sallam, M. N., Overholt, W. A., & Kairu, E. (2001). Dispersal of the exotic parasitoid Cotesia flavipes in a new ecosystem. Entomologia Experimentalis et Applicata, 98, 211–217.
Santos, R. F. D., Vacari, A. M., De Bortoli, S. A., De Bortoli, C. P., & Santos, J. A. (2015). Development of a new container for storage and release of the parasitoid Cotesia flavipes (Hymenoptera: Braconidae). Journal of Economic Entomology, 108(3), 969–974.
Shami, S., & Mohyuddin, A. I. (1992). Studies on host plant preference of Cotesia flavipes (Cameron) (Hymenoptera: Braconidae) an important parasitoid of graminaceous stalk borers. Pakistan Journal of Zoology, 24, 313–316.
Srikanth, J., & Salin, K. P. (2003). Does group size affect group dynamics of Cotesia flavipes Cameron (Hymenoptera: Braconidae) adults? Insect Environment, 9(1), 39–42.
Srikanth, J., Easwaramoorthy, S., Shanmugasundaram, M., & Kumar, R. (1999). Seasonal fluctuations of Cotesia flavipes Cameron (Hymenoptera: Braconidae) parasitism in borers of sugarcane and sorghum in Coimbatore, South India. Insect Science and its Application, 19(1), 65–74.
Srikanth, J., Easwaramoorthy, S., Kumar, R., & Shanmugasundaram, M. (2000). Patterns of Cotesia flavipes Cameron (Hymenoptera: Braconidae) parasitisation rates in sugarcane and sorghum borers in laboratory rearings. Insect Science and its Application, 20(3), 195–202.
Srikanth, J., Easwaramoorthy, S., Shanmugasundaram, M., & Kumar, R. (2003). Evaluation of two rearing methods of Cotesia flavipes Cameron (Hymenoptera: Braconidae), a parasitoid of sugarcane borers. Proceedings of Annual Convention of Sugar Technologists Association of India, 65, A75–A96.
StatSoft, Inc. (2004). STATISTICA (data analysis software system), version 7. www.statsoft.com.
Tagawa, J. (1996). Function of the cocoon of the parasitoid wasp, Cotesia glomerata L. (Hymenoptera: Braconidae): Protection against desiccation. Applied Entomology and Zoology, 31(1), 99–103.
Taneja, S. L., & Nwanze, K. F. (1991). Mass production of spotted stem borer, Chilo partellus (Swinhoe) on artificial diet. In H. David & S. Easwaramoorthy (Eds.), Biocontrol Technology for Sugarcane Pest Management (pp. 75–90). Coimbatore: Sugarcane Breeding Institute.
Tanwar, R. K., & Varma, A. (2001). Field evaluation of Cotesia flavipes Cameraon (Hymenoptera: Braconidae) (Indonesian strain) against sugarcane stalk and internode borers. Journal of Biological Control, 15(2), 127–131.
Varma, G. C., Mohanty, S. N., & Maninder, S. (1981). Natural enemies of Acigona steniella (Hmps.) (Crambidae: Lepidoptera) in the Punjab. Journal of Research Punjab Agricultural University, 18, 163–169.
Weisberg, S. (1980). Applied linear regression (1st ed., 283p). New York: Wiley.
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We thank our Director Dr. Bakshi Ram for the logistic support and academic encouragement. Two anonymous reviewers provided useful comments on the original manuscript.
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Srikanth, J., Salin, K.P., Punithavalli, M. et al. A field-release device and protocol for dispensing cocoons of Cotesia flavipes against sugarcane borers. Phytoparasitica 48, 785–800 (2020). https://doi.org/10.1007/s12600-020-00827-2
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DOI: https://doi.org/10.1007/s12600-020-00827-2