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Regulation of photosystem I-light-harvesting complex I from a red alga Cyanidioschyzon merolae in response to light intensities

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Abstract

Photosynthetic organisms use different means to regulate their photosynthetic activity in respond to different light conditions under which they grow. In this study, we analyzed changes in the photosystem I (PSI) light-harvesting complex I (LHCI) supercomplex from a red alga Cyanidioschyzon merolae, upon growing under three different light intensities, low light (LL), medium light (ML), and high light (HL). The results showed that the red algal PSI-LHCI is separated into two bands on blue-native PAGE, which are designated PSI-LHCI-A and PSI-LHCI-B, respectively, from cells grown under LL and ML. The former has a higher molecular weight and binds more Lhcr subunits than the latter. They are considered to correspond to the two types of PSI-LHCI identified by cryo-electron microscopic analysis recently, namely, the former with five Lhcrs and the latter with three Lhcrs. The amount of PSI-LHCI-A is higher in the LL-grown cells than that in the ML-grown cells. In the HL-grown cells, PSI-LHCI-A completely disappeared and only PSI-LHCI-B was observed. Furthermore, PSI core complexes without Lhcr attached also appeared in the HL cells. Fluorescence decay kinetics measurement showed that Lhcrs are functionally connected with the PSI core in both PSI-LHCI-A and PSI-LHCI-B obtained from LL and ML cells; however, Lhcrs in the PSI-LHCI-B fraction from the HL cells are not coupled with the PSI core. These results indicate that the red algal PSI not only regulates its antenna size but also adjusts the functional connection of Lhcrs with the PSI core in response to different light intensities.

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Acknowledgements

This work was supported by National Key R&D Program of China (2017YFA0503700), National Natural Science Foundation of China grant (31470339), a Strategic Priority Research Program of CAS (XDB17000000), a Key Research Program of Frontier Sciences, CAS, Grant (QYZDY-SSW-SMC003), and a grant from The Innovative Academy for Seed Design, Chinese Academy of Sciences. This work was also partially supported by Natural Science Foundation of Hebei Province (C2020205051), Doctoral fund of Hebei Normal University (L2020B20), from the Hebei Collaboration Innovation Center for Cell Signaling, China (L. Tian).

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Author notes

  1. Jie Pan

    Present address: Department of Chemistry, Michigan State University, East Lansing, MI, 48824, USA

  2. Lijing Chang and Lirong Tian have contributed equally to this work.

Authors and Affiliations

  1. Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, No, 20, Nanxincun, Xiangshan, Beijing, 100093, China

    Lijing Chang, Lirong Tian, Fei Ma, Zhiyuan Mao, Xiaochi Liu, Guangye Han, Wenda Wang, Yanyan Yang, Tingyun Kuang, Jie Pan & Jian-Ren Shen

  2. University of Chinese Academy of Sciences, Yuquan Road, Shijingshan District, Beijing, 100049, China

    Lijing Chang, Zhiyuan Mao & Xiaochi Liu

  3. Hebei Key Laboratory of Molecular and Cellular Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang, 050024, China

    Lirong Tian

  4. Research Institute for Interdisciplinary Science, Okayama University, Okayama, 700-8530, Japan

    Jian-Ren Shen

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Chang, L., Tian, L., Ma, F. et al. Regulation of photosystem I-light-harvesting complex I from a red alga Cyanidioschyzon merolae in response to light intensities. Photosynth Res 146, 287–297 (2020). https://doi.org/10.1007/s11120-020-00778-z

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