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Magnetosome mediated oral Insulin delivery and its possible use in diabetes management

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Abstract

Our study investigates the effect of magnetosome mediated oral Insulin delivery on diabetic induced rat models. The study involves the development of Magnetosome-Insulin (MI) conjugates by direct and indirect (by means of PEG) coupling method and further characterized by microscopic and spectroscopic analysis. The in vivo oral delivery of magnetosome-Insulin conjugate against streptozotocin-induced rat models and its efficiency was investigated. The impact of MI showed a remarkable change in the reduction of FBG levels up to 65% than the standard (Insulin). Similarly, the serum parameters: triglycerides (43.81%), AST&ALT (39.4 and 57.2%), total cholesterol (43.8%) showed significant changes compared to the diabetic control. The histological results of MI treated rats were found similar to control rats. Thus, these significantly notable results on diabetic rats depicts that magnetosomes can be employed as a potential approach and a very promising alternative for the parenteral route of Insulin delivery.

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Abbreviations

MTB:

Magnetotactic bacteria

DSMZ:

Deutsche Sammlung von Mikroorganismen und Zellkulturen

MSGM:

Magnetospirillum growth medium

FTIR:

Fourier-transform infrared spectroscopy

HRTEM:

High resolution transmission electron microscopy

XRD:

X-ray diffraction

HPLC:

High pressure liquid chromatography

FBG:

Fast blood glucose

STZ:

Streptozotocin

PEG:

Polyethylene glycol

MI:

Insulin magnetosome conjugate

MPI:

Insulin Magnetosome conjugate via PEG

I:

Insulin (Standard drug)

References

  1. Elena M, Matoori S, Leroux J-C. Oral delivery of macromolecular drugs: where we are after almost 100 years of attempts. Adv Drug Deliv Rev. 2016;101:108–21.

    Article  Google Scholar 

  2. Pérez YA, Urista CM, Martínez JI, Nava MDCD, Rodríguez FAR. Functionalized polymers for enhance oral bioavailability of sensitive molecules. Polymers. 2016;8(6):214.

  3. Abdul M. et al. A review on the strategies for oral delivery of proteins and peptides and their clinical perspectives. Saudi Pharm J. 2016;24.4:413–28.

    Google Scholar 

  4. Ana B. et al. Nanostructured lipid carriers: Promising drug delivery systems for future clinics. Nanomedicine. 2016;12.1:43–161.

    Google Scholar 

  5. Parveen S, Misra R, Sahoo SK. Nanoparticles: a boon to drug delivery, therapeutics, diagnostics and imaging. Nanomedicine: Nanotechnol Biol Med. 2012;8(2):147–66.

    Article  CAS  Google Scholar 

  6. Ashaben P. et al. Recent advances in protein and peptide drug delivery: a special emphasis on polymeric nanoparticles. Protein Pept Lett. 2014;21.11:1102–20.

    Google Scholar 

  7. Ahmed TA, Aljaeid BM. Preparation, characterization, and potential application of chitosan, chitosan derivatives, and chitosan metal nanoparticles in pha rmaceutical drug delivery. Drug Des Devel Ther. 2016;10:483.

  8. Faraji AH, Wipf P. Nanoparticles in cellular drug delivery. Bioorg Med Chem. 2009;17(8):2950–62.

    Article  Google Scholar 

  9. Montanari Stefano, Antonietta Gatti M. Nanopathology: the health impact of nanoparticles. CRC Press, Boca Raton, Florida, USA; 2016.

  10. Almeida AJ, Souto E. Solid lipid nanoparticles as a drug delivery system for peptides and proteins. Adv. Drug Deliv Rev. 2007;59.6:478–90.

    Article  Google Scholar 

  11. Elçioğlu HK, Sezer AD. Nanoparticle insulin drug delivery—applications and new aspects. Appl Nanotechnol Drug Deliv. 2014;4:237.

    Google Scholar 

  12. Sharma G. et al. Nanoparticle based Insulin delivery system: the next generation efficient therapy for Type 1 diabetes. J Nanobiotechnol. 2015;13.1:74

    Article  Google Scholar 

  13. Alejandro S, Augustine R. Challenges in oral drug delivery of antiretrovirals and the innovative strategies to overcome them. Adv Drug Deliv Rev. 2016;103:105–20.

    Article  Google Scholar 

  14. Yun Y, Cho WY, Park K. Nanoparticles for oral delivery: targeted nanoparticles with peptidic ligands for oral protein delivery. Adv Drug Deliv Rev. 2013;65:822–32.

    Article  CAS  Google Scholar 

  15. Hua S. et al. Advances in oral nano-delivery systems for colon targeted drug delivery in inflammatory bowel disease: selective targeting to diseased versus healthy tissue. Nanomed Nanotechnol. 2015;11.5:1117–32.

    Article  Google Scholar 

  16. Jacob JJ, Suthindhiran K. Magnetotactic bacteria and magnetosomes—scope and challenges. Mater Sci Eng C Mater. 2016;68:919–28.

    Article  CAS  Google Scholar 

  17. Dasdag S, Bektas H. Magnetotactic bacteria and their application in medicine. J Phys Chem Biophys. 2014;2(4):2161–0398.

    Google Scholar 

  18. Raguraman, V, Suthindhiran K, Comparative ecotoxicity assessment of magnetosomes and magnetite nanoparticles, Int J Environ Health Res. 2019:1–13.

  19. Hungate, RE. Chapter IV A roll tube method for cultivation of strict anaerobes. Methods Microbiol. 1969;3:117–32.

  20. Berry CC, Adam SGC. Functionalisation of magnetic nanoparticles for applications in biomedicine. J Phys D: Appl Phys. 2003;36.13:R198.

    Article  Google Scholar 

  21. Xiang L. et al. Purified and sterilized magnetosomes from Magnetospirillum gryphiswaldense MSR‐1 were not toxic to mouse fibroblasts in vitro. Lett Appl Microbiol. 2007;45.1:75–81.

    Article  Google Scholar 

  22. Frankel RB, Blakemore RP, Wolfe RS. Magnetite in freshwater magnetotactic bacteria. Science. 1979;203.4387:1355–6.

    Article  Google Scholar 

  23. Revathy T, Jayasri MA, Suthindhiran K. Toxicity assessment of magnetosomes in different models. 3 Biotech. 2017;7.2:126.

    Article  Google Scholar 

  24. Bhumkar DR, Joshi HM, Sastry M, Pokharkar VB. Chitosan reduced gold nanoparticles as novel carriers for transmucosal delivery of Insulin. Pharm. Res. 2007;24(8):1415–26.

    Article  CAS  Google Scholar 

  25. Andreani T. et al. Preparation and characterization of PEG-coated silica nanoparticles for oral Insulin delivery. Int J Pharm. 2014;473.1-2:627–35.

    Article  Google Scholar 

  26. Piyasi M. et al. pH-sensitive chitosan/alginate core-shell nanoparticles for efficient and safe oral Insulin delivery. Int J Biol Macromol. 2015;72:640–8.

    Article  Google Scholar 

  27. Unnikrishnan PS, Jayasri MA. Antidiabetic studies of Chaetomorpha antennina extract using experimental models. J Appl Psychol. 2017;29.2:1047–56.

    Google Scholar 

  28. Hinds KD, Kim SW. Effects of PEG conjugation on Insulin properties. Adv Drug Deliv Rev. 2002;54:505–30.

    Article  CAS  Google Scholar 

  29. Marlene L. et al. Dual chitosan/albumin-coated alginate/dextran sulfate nanoparticles for enhanced oral delivery of Insulin. J Control Release. 2016;232:29–41.

    Article  Google Scholar 

  30. Pedro F. et al. Polymer-based nanoparticles for oral Insulin delivery: revisited approaches. Biotechnol Adv. 2015;33.6:1342–54.

    Google Scholar 

  31. Meredith HL, Lowman AM. Biodegradable nanoparticles for drug delivery and targeting. Curr Opin Solid State Mater Sci. 2002;6:319–27.

    Article  Google Scholar 

  32. Chen M-C. et al. A review of the prospects for polymeric nanoparticle platforms in oral Insulin delivery. Biomaterials. 2011;32:9826–38.

    Article  CAS  Google Scholar 

  33. Moussa BA, Farouk F, Azzazy HME. A validated RP-HPLC method for the determination of recombinant human Insulin in bulk and pharmaceutical dosage form. J Chem. 2010;7(S1):S449–57.

    CAS  Google Scholar 

  34. Singh MA. Magnetotactic bacteria: nanodrivers of the future. Crit Rev Biotechnol. 2016;36.5:788–802.

    Google Scholar 

  35. Jing X, Li A, Li J. Advances in pH‐sensitive polymers for smart insulin delivery. Macromol Rapid Commun. 2017;38.23:1700413.

    Google Scholar 

  36. Rinku DU, Paknikar KM. Zinc oxide nanoparticles show antidiabetic activity in streptozotocin-induced Type 1 and 2 diabetic rats. Nanomedicine. 2014;9.1:89–104.

    Google Scholar 

  37. Naskar S, Sharma S, Koutsu K. Chitosan-based nanoparticles: an overview of biomedical applications and its preparation. J Drug Deliv Sci Technol. 2019;49:66–81.

    Article  CAS  Google Scholar 

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Acknowledgements

This work was supported by VIT University. The authors thank the management for providing the facilities for the research.

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Authors and Affiliations

  1. Marine Biotechnology and Bioproducts Lab, School of Biosciences and Technology, Vellore Institute of Technology, Vellore, Tamilnadu, 632014, India

    Varalakshmi Raguraman, M. A. Jayasri & K. Suthindhiran

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  1. Varalakshmi Raguraman
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  2. M. A. Jayasri
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  3. K. Suthindhiran
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Correspondence to K. Suthindhiran.

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Raguraman, V., Jayasri, M.A. & Suthindhiran, K. Magnetosome mediated oral Insulin delivery and its possible use in diabetes management. J Mater Sci: Mater Med 31, 75 (2020). https://doi.org/10.1007/s10856-020-06417-2

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