A modular microarray imaging system for highly specific COVID-19 antibody testing

Per Niklas Hedde; Timothy J. Abram; Aarti Jain; Rie Nakajima; Rafael Ramiro de Assis; Trevor Pearce; Algis Jasinskas; Melody N. Toosky; Saahir Khan; Philip L. Felgner; Enrico Gratton; Weian Zhao

doi:10.1039/D0LC00547A

A modular microarray imaging system for highly specific COVID-19 antibody testing†

Per Niklas Hedde,

*^abc Timothy J. Abram,^d Aarti Jain,^e Rie Nakajima,^e Rafael Ramiro de Assis,^e Trevor Pearce,^b Algis Jasinskas,^e Melody N. Toosky,^d Saahir Khan,

^f Philip L. Felgner,^e Enrico Gratton^bc and Weian Zhao

^abghij

Author affiliations

* Corresponding authors

^a Department of Pharmaceutical Sciences, University of California, Irvine, Irvine, CA, USA
E-mail: phedde@uci.edu

^b Department of Biomedical Engineering, University of California, Irvine, Irvine, CA, USA

^c Laboratory for Fluorescence Dynamics, University of California, Irvine, Irvine, CA, USA

^d Velox Biosystems, Irvine, CA, USA

^e Department of Physiology and Biophysics, University of California, Irvine, Irvine, CA, USA

^f Division of Infectious Diseases, Department of Medicine, University of California Irvine Health, Orange, CA, USA

^g Sue and Bill Gross Stem Cell Research Center, University of California, Irvine, Irvine, CA, USA

^h Chao Family Comprehensive Cancer Center, University of California, Irvine, Irvine, CA, USA

ⁱ Edwards Life Sciences Center for Advanced Cardiovascular Technology, University of California, Irvine, Irvine, CA, USA

^j Department of Biological Chemistry, University of California, Irvine, Irvine, CA 92697, USA

Abstract

To detect the presence of antibodies in blood against SARS-CoV-2 in a highly sensitive and specific manner, here we describe a robust, inexpensive ($200), 3D-printable portable imaging platform (TinyArray imager) that can be deployed immediately in areas with minimal infrastructure to read coronavirus antigen microarrays (CoVAMs) that contain a panel of antigens from SARS-CoV-2, SARS-1, MERS, and other respiratory viruses. Application includes basic laboratories and makeshift field clinics where a few drops of blood from a finger prick could be rapidly tested in parallel for the presence of antibodies to SARS-CoV-2 with a test turnaround time of only 2–4 h. To evaluate our imaging device, we probed and imaged coronavirus microarrays with COVID-19-positive and negative sera and achieved a performance on par with a commercial microarray reader 100× more expensive than our imaging device. This work will enable large scale serosurveillance, which can play an important role in the months and years to come to implement efficient containment and mitigation measures, as well as help develop therapeutics and vaccines to treat and prevent the spread of COVID-19.

This article is part of the themed collections: Coronavirus articles - free to access collection and Coronavirus collection – Analytical

Lab on a Chip

A modular microarray imaging system for highly specific COVID-19 antibody testing†

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A modular microarray imaging system for highly specific COVID-19 antibody testing

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