Skip to main content
SpringerLink
Log in

Specifics of Quantitation of Exogenous cis Fatty Acids in Biological Objects

  • PHYSICOCHEMICAL PROCESSES AT THE INTERFACES
  • Published:
Protection of Metals and Physical Chemistry of Surfaces Aims and scope Submit manuscript

Abstract

A method for quantitation of exogenous cis fatty acids in the presence of exogenous cis and trans fatty acids that does not require time-consuming and tedious chromatographic separation is described. In it, we use an inhibitor that blocks Elovl6, an enzyme involved in fatty acid synthesis in vivo. Samples prepared in the absence or presence of the inhibitor are sequentially analyzed by liquid chromatography with mass spectrometric detection. The developed method is used for analyzing endogenous cis fatty acids synthesized in cancer cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.

Similar content being viewed by others

REFERENCES

  1. Christie, W.W., Lipids, 1998, vol. 33, no. 4, p. 343.

    Article  CAS  Google Scholar 

  2. Zelles, L., Biol. Fertil. Soils, 1999, vol. 29, no. 2, p. 111.

    Article  CAS  Google Scholar 

  3. Jemal, A., Bray, F., Ward, E., et al., Ca-Cancer J. Clin., 2011, vol. 61, no. 2, p. 69.

    Article  Google Scholar 

  4. Slattery, M.L., Benson, J., Ma, K., et al., Nutr. Cancer, 2001, vol. 39, no. 2, p. 170.

    Article  CAS  Google Scholar 

  5. Gebauer, S.K., Chardigny, J.M., Jakobsen, M.U., et al., Adv. Nutr., 2011, vol. 2, no. 4, p. 332.

    Article  CAS  Google Scholar 

  6. Liu, W.H., Inbaraj, B.S., and Chen, B.H., Food Chem., 2007, vol. 104, no. 4, p. 1740.

    Article  CAS  Google Scholar 

  7. Katan, M.B., Zock, P.L., and Mensink, R.P., Annu. Rev. Nutr., 1995, vol. 15, p. 473.

    Article  CAS  Google Scholar 

  8. Costa, T.G., Castilho, H.S., and Sanches, S.A., Trends Food Sci. Technol., 2011, vol. 22, p. 543.

    Article  Google Scholar 

  9. Roach, J.A., Mossobaa, M.M., Yurawecza, M.P., et al., Anal. Chim. Acta, 2002, vol. 465, p. 207.

    Article  CAS  Google Scholar 

  10. Berdeaux, O., Dutta, P.C., Dobarganes, M.C., et al., Food Anal. Methods, 2009, vol. 2, p. 30.

    Article  Google Scholar 

  11. Zhang, M., Yang, X., Zhao, H.T., et al., Food Control, 2015, vol. 57, p. 293.

    Article  CAS  Google Scholar 

  12. Kulsing, C., Nolvachai, Y., Zeng, A.X., et al., ChemPlusChem, 2014, vol. 6, no. 79, p. 790.

    Article  Google Scholar 

  13. Christie, W.W. and Grace, H.M., J. Chromatogr. A, 1989, vol. 469, p. 261.

    Article  CAS  Google Scholar 

  14. Kraft, J., Kramer, J.K., Hernandez, G., et al., Lipid Technol., 2014, vol. 2, no. 26, p. 39.

    Article  Google Scholar 

  15. Stolyhwo, A. and Rutkowska, J., Food Anal. Methods, 2013, vol. 6, p. 457.

    Article  Google Scholar 

  16. Kyle, J.E., Zhang, X., Weitz, K.K., et al., Analyst, 2016, vol. 5, no. 141, p. 1649.

    Article  Google Scholar 

  17. Damen, C.W., Isaac, G., Langridge, J., et al., J. Lipid Res., 2014, vol. 22, p. 382.

    Google Scholar 

  18. Zakharova, G.S., Poloznikov, A.A., Astakhova, L.A., et al., Izv. Akad. Nauk, Ser. Khim., no. 12, p. 2307.

  19. Mutay, A., Özcan, F., Aslan, I., et al., Lipids Health Dis., 2013, vol. 169, no. 3, p. 189.

    Google Scholar 

  20. Gutiérrez-Juárez, R., Pocai, A., and Mulas, C., J. Clin. Invest., 2006, vol. 169, p. 1686.

    Article  Google Scholar 

  21. Moon, Y.A., Shah, N.A., Mohapatra, S., et al., J. Biol. Chem., 2001, vol. 276, p. 45358.

    Article  CAS  Google Scholar 

  22. Matsuzaka, T., Shimano, H., Yahagi, N., et al., J. Lipid Res., 2002, vol. 43, p. 911.

    CAS  Google Scholar 

  23. Green, C.D., Ozguden-Akkoc, C.G., Wang, Y., et al., J. Lipid Res., 2010, vol. 51, p. 1871.

    Article  CAS  Google Scholar 

  24. Shi, H.B., Wu, M., Zhu, J.J., and Zhang, C.H., J. Dairy Sci., 2017, vol. 100, p. 4987.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. A.N. Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, Moscow, Russia

    Z. B. Khesina, S. D. Iartsev & A. K. Buryak

Authors
  1. Z. B. Khesina
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. D. Iartsev
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. K. Buryak
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Z. B. Khesina.

Additional information

Translated by A. Kukharuk

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Khesina, Z.B., Iartsev, S.D. & Buryak, A.K. Specifics of Quantitation of Exogenous cis Fatty Acids in Biological Objects. Prot Met Phys Chem Surf 56, 480–484 (2020). https://doi.org/10.1134/S207020512003017X

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S207020512003017X

Search

Navigation