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Influences of Regulation of miR-126 on Inflammation,Th17/Treg Subpopulation Differentiation, and Lymphocyte Apoptosis through Caspase Signaling Pathway in Sepsis

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Abstract

To observe the inflammatory response, differentiation of Th17/Treg subsets and apoptosis of lymphocytes, by regulating miR-126 in lymphocytes of septic rats. After using cecal ligation and puncture to establish sepsis model, miR-126 mimic and miR-126 inhibitor were used to transfect lymphocytes of septic rats in vitro and in vivo. ELISA was used to detect TNF-α, IL-6, IL-17, and IL-10, the differentiation of Th17 and Treg was measured by flow cytometry, and apoptosis of lymphocytes was observed by fluorescence microscope; the changes of caspase signaling pathway were detected by immunofluorescence, PCR, and Western blotting. The result show that the expression of miR-126 increased in sepsis. After overexpression of miR-126, the release of TNF-α, IL-6, and IL-17 decreased; the release of IL-10 increased; T lymphocyte subsets differentiated toward Treg; caspase signaling pathway weakened; and lymphocyte of apoptosis decreased compared with sepsis group. While, after inhibition of miR-126, the release of TNF-α, IL-6, and IL-17 increased; the release of IL-10 decreased; T lymphocyte subsets differentiated toward TH17; caspase signaling pathway enhanced; and lymphocyte of apoptosis increased compared with sepsis group. Taken together, regulation of miR-126 can alter the inflammatory response, differentiation of T lymphocyte subsets, and apoptosis of lymphocytes in septic rats.

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Acknowledgments

We acknowledge the financial support of the Natural Science Research Projects in Universities of Anhui Province (KJ2018A0244) and Natural Science Research Projects in Universities of Anhui Province (KJ2019A0351).

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  1. Qi Zou and Mei Yang contributed equally to this work.

Authors and Affiliations

  1. Department of Critical Care Medicine, The first Affiliated Hospital of Bengbu Medical College, Anhui, 233004, Bengbu, People’s Republic of China

    Qi Zou & Cheng Liu

  2. Department of Pediatrics, The first Affiliated Hospital of Bengbu Medical College, Anhui, 233004, Bengbu, People’s Republic of China

    Mei Yang

  3. Department of Pharmacy, The first Affiliated Hospital of Bengbu Medical College, Anhui, 233004, Bengbu, People’s Republic of China

    Meiling Yu

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  1. Qi Zou
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Correspondence to Cheng Liu.

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Zou, Q., Yang, M., Yu, M. et al. Influences of Regulation of miR-126 on Inflammation,Th17/Treg Subpopulation Differentiation, and Lymphocyte Apoptosis through Caspase Signaling Pathway in Sepsis. Inflammation 43, 2287–2300 (2020). https://doi.org/10.1007/s10753-020-01298-7

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