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Halomonas marinisediminis sp. nov., A Moderately Halophilic Bacterium Isolated from the Bohai Gulf

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Abstract

A novel Gram-stain-negative, moderately halophilic bacterium, designated strain 204T, was isolated from a marine sediment sample in the Bohai Gulf, Yellow Sea, China. Cells of strain 204T are aerobic, motile, cocci or short rods with two lateral flagella. Growth was observed at 15–40 °C (optimum, 30 °C), pH 6.0–9.0 (optimum, 7.0–7.5) and in the presence of 1.0–18.0% (w/v) NaCl (optimum, 3.0–8.0%). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 204T belonged to the genus Halomonas, showing highest sequence similarity to Halomonas alimentaria YKJ-16T (98.08%), followed by Halomonas sediminicola (97.47%), Halomonas fontilapidosi (97.14%), Halomonas halodenitrificans (96.98%), Halomonas ventosae (96.92%), and Halomonas shengliensis (96.85%). The draft genome is 3.8 Mb in length, containing 3673 protein-coding genes, 62 tRNA genes and 10 rRNA genes, and DNA G+C content is 62.7 mol%. Strain 204T contains phosphatidylglycerol, phosphatidylethanolamine, and diphosphatidylglycerol as the main polar lipids, and the predominant respiratory quinone was ubiquinone Q-9. The major cellular fatty acids (> 5%) are C18:1ω7c, C16:1ω7c and/or C16:1ω6c, C16:0 and C12:03-OH. Strain 204T was clearly distinguished from the closely related type strains through phylogenetic analysis, dDNA-DNA hybridization, fatty acid composition data and a range of physiological and biochemical characteristics comparisons. On the basis of the polyphasic analysis, strain 204T is considered to represent a novel species of the genus Halomonas, for which the name Halomonas marinisediminis sp. nov. is proposed. The type strain is 204T (= MCCC 1H00366T = KCTC 62957T).

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Acknowledgements

We sincerely thank Prof. Aharon Oren in Hebrew University of Jerusalem for advice on nomenclature. This work was supported by the National Natural Science Foundation of China (81702054, 81672044, 81501718) and Natural Science Foundation of Shandong Province (ZR2017BC011).

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Correspondence to Boqing Li or Xiaofei Ji.

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The GenBank accession numbers of Halomonas marinisediminis 204T are MK347065 (16S rRNA gene) and SLTR00000000 (draft genome), respectively. The Digital Protologue database (DPD) number for strain 204T is TA00990

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Zhao, H., Wang, C., Tian, Y. et al. Halomonas marinisediminis sp. nov., A Moderately Halophilic Bacterium Isolated from the Bohai Gulf. Curr Microbiol 77, 3773–3779 (2020). https://doi.org/10.1007/s00284-020-02147-4

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  • DOI: https://doi.org/10.1007/s00284-020-02147-4

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