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Arthrobacter crusticola sp. nov., Isolated from Biological Soil Crusts in the Mu Us Sandy Land, China

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Abstract

Mu Us Sandy Land in China is a very fragile ecological environment due to serious desertification. While attempting to gain insights into the biodiversity of biological soil crusts of Mu Us Sandy Land, a novel bacterial strain, SLN-3T, was isolated. It was phylogenetically placed into the genus Arthrobacter within the family Micrococcaceae based on its 16S rRNA gene sequence. The most closely related species were Arthrobacter ruber MDB1-42T (98.6%) and Arthrobacter agilis DSM 20550T (98.3%). Cells of the novel species were Gram-stain-positive, aerobic, and non-endospore-forming. The values of average nucleotide identity and the digital DNA-DNA hybridization between SLN-3T and MDB1-42T were 84.9% and 21.3%, respectively. The draft genome size of strain SLN-3T was 3.67 Mb, and its genomic G+C content was 68.1%. The predominant cellular fatty acids were anteiso-C15:0 and C17:0 anteiso. Glucose, galactose, and ribose were the whole-cell sugars. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, glycolipid, and phospholipid. The peptidoglycan contained lysine, glutamic acid, and alanine. The predominant menaquinone was MK-9(H2). Based on the data from the chemotaxonomic, phylogenetic, and phenotypic evidence, a novel species named Arthrobacter crusticola sp. nov is proposed, whose type strain is SLN-3T (= ACCC 61595T = JCM 33723T).

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Funding

This work was supported by the State Key Research and Development Program of China (2016YFC0500801). National Natural Science Foundation of China (31800522).

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Authors and Affiliations

  1. State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, People’s Republic of China

    Lei Liu, Lixiong Liang, Lijun Xu, Miao Chi, Shaowen Shi & Lubin Li

  2. Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, People’s Republic of China

    Shanwen He & XiaoXia Zhang

  3. Beijing Botanical Garden, Beijing, 100093, People’s Republic of China

    Miao Chi

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  1. Lei Liu
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Contributions

LL conceived the study, participated in its design, and coordinated and drafted the manuscript; LL, SH, LX, MC and SS participated in the design and coordination of the study and performed the measurement; XZ and LL conceived the study, participated in its design, and coordinated and drafted the manuscript. All authors read and approved the final manuscript.

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Correspondence to XiaoXia Zhang or Lubin Li.

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Liu, L., Liang, L., He, S. et al. Arthrobacter crusticola sp. nov., Isolated from Biological Soil Crusts in the Mu Us Sandy Land, China. Curr Microbiol 77, 2042–2048 (2020). https://doi.org/10.1007/s00284-020-02070-8

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