Abstract
A novel yeast species of Starmerella vitis f.a. sp. nov. is proposed to accommodate five strains isolated from flowers, grapes and an insect in the Azores, Canada, Hungary, Palau and Taiwan. As the strains were genetically distinct, we used parsimony network analysis based on ITS-D1/D2 sequences to delineate the species in a statistically objective manner. According to sequence comparisons and phylogenetic analysis, the novel species is most closely related to Starmerella lactis-condensi. The two species cannot be distinguished by conventional physiological tests. The type strain of Starmerella vitis f.a., sp. nov. is CBS 16418T; Mycobank number MB 835251.
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Funding
This work was partly funded by the Slovenian Research Agency (P4-0116 and MRIC-UL ZIM, IP-0510) (N.Č.), the State Secretariat for Education of the Hungarian Ministry of Human Capacities and by the European Union and co-financed by the European Social Fund (Grant Agreement No. EFOP-3.6.3-VEKOP-16-2017-00005) (D.D. and G.P.).
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NČ wrote the manuscript, analysed the data and prepared the figures, GP and M-AL contributed to text preparation and revised the manuscript, JD-N, RF-D, MS, M-AL, GP and DD isolated, identified and deposited the strains, DS conceptualized the study, TL and CP supervised JD-N. and RF-D. All authors read and approved the final version of the manuscript.
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Dorit Schuller: Department of Biology, Centre of Molecular and Environmental Biology (CBMA), University of Minho, Braga, Portugal until 2013.
The GenBank/EMBL/DDBJ accession numbers of the LSU and ITS sequences of Starmerella vitis sp. nov. CBS 16418T are KC992848 and MN317383, respectively. The MycoBank accession numbers is MB 835251.
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Čadež, N., Drumonde-Neves, J., Sipiczki, M. et al. Starmerella vitis f.a., sp. nov., a yeast species isolated from flowers and grapes. Antonie van Leeuwenhoek 113, 1289–1298 (2020). https://doi.org/10.1007/s10482-020-01438-x
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DOI: https://doi.org/10.1007/s10482-020-01438-x