Abstract
A new fluorescence turn-on sensing platform has been developed applicable for sensitive profiling of multiple chemical and biological analytes, using azobenzene-quantum dot as a new stimuli-responsive optical nanoprobe. An azobenzene-carrying compound bis [4, 4′-(dithiophenyl azo)-1, 3-benzenediamine] (DTPABDA) is for the first time reported to be used for conjugation with CdSe/ZnS core/shell quantum dots (QDs) via the ligand exchange reaction. Due to the photo-induced electron-transfer (PET) effect, the electron-withdrawing azobenzene groups of DTPABDA can significantly cause the photoluminescence (PL) of QDs quenched. The QDs’ PL can be subsequently reignited by the removal of azo moiety cleavable through three types of specific reactions: the dithionite reduction, hypochlorite oxidation, and azoreductase enzymatic catalysis, respectively. By monitoring of reaction-induced recovery of FL signals at 560 nm with an excitation of 450 nm, such azobenzene-QDs conjugates served as a new nanoprobe enabling the fluorescence turn-on sensing of dithionite, hypochlorite, and azoreductase with high sensitivity, broad linear range, and good selectivity. The successful detection of target analytes in real samples reveals the potential of our method in practical applications, such as biosensing, environmental and industrial monitoring.
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Funding
This work was supported by the Guangdong Basic and Applied Basic Research Foundation (2020A1515010957), the Fundamental Research Funds for the Central Universities (No. 21618414), the Open Funds of the State Key Laboratory of Electroanalytical Chemistry (No. SKLEAC201903), and the Guangdong Innovative and Entrepreneurial Research Team Program (No. 2014ZT05S136).
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Zha, Y., Xin, R., Zhang, M. et al. Stimuli-responsive azobenzene-quantum dots for multi-sensing of dithionite, hypochlorite, and azoreductase. Microchim Acta 187, 481 (2020). https://doi.org/10.1007/s00604-020-04455-9
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DOI: https://doi.org/10.1007/s00604-020-04455-9