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Enhanced exopolysaccharide production in submerged fermentation of Ganoderma lucidum by Tween 80 supplementation

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Abstract

Bioactive polysaccharides extracted from Ganoderma lucidum (G. lucidum) have been widely applied in food and medicine for their multiple functions. In this study, G. lucidum exopolysaccharide (EPS) production in submerged fermentation was stimulated by Tween 80. The addition of 0.25% Tween 80 on day 3 gave a maximum production of mycelial biomass and EPS, with an increase of 19.76 and 137.50%, respectively. Analysis of fermentation kinetics showed that glucose was consumed faster after adding Tween 80, while the expression of EPS biosynthesis-related genes and ATP generation were greatly improved. Moreover, Tween 80 resulted in the significant accumulation of reactive oxygen species and increased cell membrane and cell wall permeability. The EPS from Tween 80-containing medium had higher contents of carbohydrate and uronic acid, lower molecular weight, and higher antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals than those of EPS produced in the absence of Tween 80. This study provides further evidence to clarify the stimulatory effects of Tween 80 in fermentation and provides a guide for the production of bioactive G. lucidum EPS.

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Acknowledgements

This work was supported by the Key-Area Research and Development Program of Guangdong Province (No. 2018B020205001), the Guangdong Province Innovation Team Construction Program on Modern Agriculture Industrial Technology System (The Edible Fungus) (No. 2019KJ103), the Guangdong Province Science and Technology Project (No. 2019A050520003), and the Strategic Priority Research Program of the Chinese Academy of Sciences (No. XDA01020304).

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  1. Xiaobing Yang and Yingyin Yang are the co-first authors and have the equal contributions.

Authors and Affiliations

  1. Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, 510070, China

    Xiaobing Yang, Yingyin Yang, Yifan Zhang, Jiahao He & Yizhen Xie

  2. Yuewei Edible Fungi Technology Co. Ltd., Guangzhou, 510663, China

    Yizhen Xie

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  1. Xiaobing Yang
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Yang, X., Yang, Y., Zhang, Y. et al. Enhanced exopolysaccharide production in submerged fermentation of Ganoderma lucidum by Tween 80 supplementation. Bioprocess Biosyst Eng 44, 47–56 (2021). https://doi.org/10.1007/s00449-020-02418-1

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