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A Potentiometric Sensor Based on a Copper(II) Ion Composite for Vitamin C Determination in Urine

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Abstract

We developed an all-solid-state sensor for monoascorbate ion based on a composite containing copper(II) ions (5 wt %), graphite and epoxy resin. The sensor presented super-Nernstian response at room temperature under continuous bubbling of oxygen gas in sample. The detection limit of the sensor was 0.02 mM and its linear range was between 0.05 and 1.5 mM with upper limit of quantification equal to 3 mM. The leaching out effect of the active component from composite was estimated less than 11 µg of copper(II) sulfate per day under continuous diffusion process in deionized water. Photomicrographs of the electrode surface did not show the presence of salt particles on the surface indicating that copper(II) ions could be intercalated in graphene layers. Attenuated total reflectance infrared spectrum demonstrated this possibility. The sensor was successfully applied to the in vitro determination of L-ascorbic acid in urine.

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ACKNOWLEDGMENTS

We acknowledge Prof. Dr. Norberto Sanches Gonçalves from UNIFESP (Diadema Campus) for obtaining infrared spectrum by attenuated total reflectance (ATR) of our compounds and the Centre of Electronic Microscopy from UNIFESP (hhtp://www.unifesp.br/centros/ceme/links. htm) for obtaining photomicrographs.

Funding

This research was supported in part by FAPESP (2007/556277 and 2014/04596-8) and CNPq (124214/2013-4) through a scholarship to K.B.R.

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Authors and Affiliations

  1. Clinical Laboratory, Department of Clinical Medicine, Faculty of ABC Medicine 821, Príncipe de Gales Avenue, 09060-650, Santo André, SP, Brazil

    Kelly B. Rodrigues & Fernando L. A. Fonseca

  2. The Municipal University of São Caetano do Sul 50, Santo Antônio Street, 09521-160, São Caetano do Sul, SP, Brazil

    Kelly B. Rodrigues & Julio C. B. Fernandes

  3. Federal University of Western Pará – UFOPA, Institute of Educational Sciences S/N Marechal Rondon, Caranazal, CEP 68040-070, Santarém, PA, Brazil

    Julio C. B. Fernandes

Authors
  1. Kelly B. Rodrigues
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  2. Fernando L. A. Fonseca
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  3. Julio C. B. Fernandes
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Corresponding author

Correspondence to Julio C. B. Fernandes.

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Rodrigues, K.B., Fonseca, F.L. & Fernandes, J.C. A Potentiometric Sensor Based on a Copper(II) Ion Composite for Vitamin C Determination in Urine. J Anal Chem 75, 1086–1093 (2020). https://doi.org/10.1134/S1061934820080122

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