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Fabrication of an Impedimetric Immunosensor for Screening and Determination of Vincristine in Biological Samples

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Abstract

Terpenoids of medicinal plants are one of the most important sources of therapeutic drugs. Among them, Vincristine (VCR) is prescribed for a wide variety of cancer types. Therefore, development of sensitive methods for VCR determination is urgently required. Here, a label-free and highly sensitive impedimetric biosensor was fabricated for electrochemical detection of VCR. First, multi-wall carbon nano tubes (MWCNTs) were dropped on the surface of a glassy carbon electrode. Then, the VCR monoclonal antibody (Ab) was covalently immobilized on the MWCNTs via carbodiimide coupling reaction using N-(3-dimethylaminopropyl)-N'-ethyl carbodiimide and N-hydroxy succinimide. The step-by-step modification process was characterized by electrochemical impedance spectroscopy and cyclic voltammetry in presence of a redox probe [Fe(CN)6]3–/4–. The VCR concentration was measured through increasing impedance values in the corresponding specific binding of VCR to the Ab. The increased electron-transfer resistance (Ret) values were proportional to the concentration of VCR and the biosensor detected concentrations varied in the range of 0.2–50 nM with a detection limit of 8 × 10–2 nM (S/N = 3). Practical analytical utility of the proposed method was demonstrated by VCR determination in medicinal plant extracts and human serum samples with satisfactory recoveries. The proposed biosensor may be a promising alternative for screening of medicinal plants to produce VCR and for the determination of drug concentration in cancerous potions.

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Funding

The authors acknowledge the University of Bu Ali Sina for financial support (grant no. 1395, 6).

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Authors and Affiliations

  1. Department of Biotechnology, Bu-Ali Sina University, Hamadan, Iran

    Haidar Saify Nabiabad &  Massoumeh Amini

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  1. Haidar Saify Nabiabad
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  2. Massoumeh Amini
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Correspondence to Haidar Saify Nabiabad.

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Haidar Saify Nabiabad, Massoumeh Amini Fabrication of an Impedimetric Immunosensor for Screening and Determination of Vincristine in Biological Samples. J Anal Chem 75, 1094–1101 (2020). https://doi.org/10.1134/S1061934820080092

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  • DOI: https://doi.org/10.1134/S1061934820080092

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