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Derivatization Method for the Determination of Amino Acids in Tobacco by Gas Chromatography–mass Spectrometry

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Abstract

In this paper, we evaluated the use of a silylation reagent N-methyl-N-(tert-butyldimethylsilyl)trifluoroacetamide in the microwave-assisted derivatization method to replace a traditional prolonged heating method for the determination of 12 amino acids. Several parameters, including the kind of extraction solvent, extraction time, microwave power and microwave time, were optimized. The experiment showed that the optimal derivatization time was 3 min with a microwave power of 240 W. The calibration curves were linear with the correlation coefficients ranging from 0.995 to 1.000 in the concentration levels between 0.1–20 μg/mL and the relative standard deviations were between 4.4 and 18%. The limits of detection were 0.1–2.4 μg/mL and the recoveries of those compounds were 62–115%. A comparison of this method to other methods has demonstrated that the proposed method is a rapid and reliable sample-treatment procedure for amino acids determination in real tobacco samples.

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REFERENCES

  1. Zhu, F.L., Wang, J., Zhu, L.J., Tan, L.L., Feng, G.L., Liu, S.M., Dai, Y., and Wang, H., Talanta, 2013, vol. 150, p. 388.

    Article  Google Scholar 

  2. Sugimura, T., Kawachi, T., Nagao, M., Yahagi, T., Seino, Y., Okamoto, T., Shudo, K., Kosuge, T., Tsuji, K., Wakabayashi, K.J., Iitaka, Y., and Itai, A., Proc. Jpn. Acad.,Ser. B, 1977, vol. 53, no. 1, p. 58.

    CAS  Google Scholar 

  3. Yamamoto, T., Tsuji, K., Kosuge, T., Okamoto, T., Shudo, K., Takeda, K., Iitaka, Y., Yamaguchi, K., Seino, Y., Yahagi, T., Nagao, M., and Sugimura, T., Proc. Jpn. Acad.,Ser. B, 1978, vol. 54, no. 5, p. 248.

    CAS  Google Scholar 

  4. Jean, D.B., Chem. Ind., 1960, vol. 93, no. 8, p. 201.

    Google Scholar 

  5. Metrione, R.M., J. Chromatogr. A, 1986, vol. 363, no. 2, p. 337.

    Article  CAS  Google Scholar 

  6. Ali, H., Pätzold, R., and Brückner, H., Food Chem., 2006, vol. 99, no. 4, p. 803.

    Article  CAS  Google Scholar 

  7. Cheng, Y., Chen, L., and Deng, X.C., Tob. Sci. Technol., 2010, vol. 47, no. 10, p. 35.

    Google Scholar 

  8. Palmeira-de-Oliveira, A., Passarinha, L.A., Gaspar, C., Palmeira-de-Oliveira, R., Sarmento, B., Martinez-de-Oliveira, J., Pina-Vaz, C., Rodrigues, A.G., and Queiroz, J.A., J. Chromatogr. B: Anal. Technol. Biomed. Life Sci., 2011, vol. 879, no. 31, p. 3749.

    Article  CAS  Google Scholar 

  9. Chen, F., Wang, S., Guo, W.X., and Hu, M.L., Talanta, 2005, vol. 66, no. 3, p. 755.

    Article  CAS  Google Scholar 

  10. Mudiam, M.K.R. and Ratnasekhar, Ch., J. Chromatogr. A, 2013, vol. 1291, p. 10.

    Article  CAS  Google Scholar 

  11. Li, G., Xie, W.Y., Sha, Y.F., Lin, H.Q., and Liu, B.Z., J. Chromatogr. A, 2013, vol. 1296, p. 243.

    Article  CAS  Google Scholar 

  12. Shamsipur, M., Naseri, M.T., and Babri, M., J. Pharm. Biomed. Anal., 2013, vols. 81–82, no. 7, p. 65.

    Article  Google Scholar 

  13. Mudiam, M.K.R., Ratnasekhar. C., Jain, R., Saxena, P.N., Chauhan, A., and Murthy, R.C., J. Chromatogr. B: Anal. Technol. Biomed. Life Sci., 2012, vol. 907, p. 56.

    Article  CAS  Google Scholar 

  14. Zhang, X., Yang, L., and Mester, Z., Anal. Chim. Acta, 2012, vol. 744, no. 18, p. 54.

    Article  CAS  Google Scholar 

  15. Pereira, D.M., Vinholes, J., Guedes de Pinho, P., Valentão, P., Mouga, T., Teixeira, N., and Andrade, P.B., Talanta, 2012, vol. 100, no. 20, p. 391.

    Article  CAS  Google Scholar 

  16. Kaspar, H., Dettmer, K., Chan, Q., Daniels, S., Nimkar, S., Daviglus, M.L., Stamler, J., Elliott, P., and Oefner, P.J., J. Chromatogr. B: Anal. Technol. Biomed. Life Sci., 2009, vol. 877, nos. 20–21, p. 1838.

    Article  CAS  Google Scholar 

  17. Kaspar, H., Dettmer, K., Gronwald, W., and Oefner, P.J., J. Chromatogr. B: Anal. Technol. Biomed. Life Sci., 2008, vol. 870, no. 2, p. 222.

    Article  CAS  Google Scholar 

  18. Kawana, S., Nakagawa, K., Hasegawa, Y., and Yamaguchi, S., J. Chromatogr. B: Anal. Technol. Biomed. Life Sci., 2010, vol. 878, no. 30, p. 3113.

    Article  CAS  Google Scholar 

  19. Liu, R., Hou, X., Lv, Y., McCooeye, M., Yang, L., and Mester, Z., Anal. Chem., 2013, vol. 85, no. 8, p. 4087.

    Article  CAS  Google Scholar 

  20. Kvitvang, H.F.N., Andreassen, T., Adam, T., Villas-Bôas, S.G., and Bruheim, P., Anal. Chem., 2011, vol. 83, no. 7, p. 2705.

    Article  CAS  Google Scholar 

  21. Jiye, A., Gullberg, J., Johansson, A.I., Jonsson, P., Antti, H., Marklund, S.L., and Moritz, T., Anal. Chem., 2005, vol. 77, no. 24, p. 8086.

    Article  Google Scholar 

  22. De Paiva, .M.J., Menezes, H.C., Christo, P.P., Resende, R.R., and Cardeal, Z.L., J. Chromatogr. B: Anal. Technol. Biomed. Life Sci., 2013, vol. 931, no. 14, p. 97.

  23. Leggio, A., Belsito, E.L., De Marco, R., Liguori, A., Siciliano, C., and Spinella, M., J. Chromatogr. A, 2012, vol. 1241, no. 11, p. 96.

    Article  CAS  Google Scholar 

  24. Pinto, M.C., Nunes de Paiva, M.J., Oliveira-Lima, O.C., Costa Menezes, H., de Lourdes Cardeal, L., Vinícius Gomez, M., Ribeiro Resende, R., and Santiago Gomez, R., J. Chem. Neuroanat., 2014, vol. 55, no. 2, p. 24.

    Article  CAS  Google Scholar 

  25. Jiménez-Martín, E., Ruiz, J., Pérez-Palacios, T., Silva, A., and Antequera, T., J. Agric. Food Chem., 2012, vol. 60, no. 10, p. 2456.

    Article  Google Scholar 

  26. Christou, C., Gika, H.G., Raikos, N., and Theodoridis, G., J. Chromatogr. B: Anal. Technol. Biomed. Life Sci., 2014, vol. 964, p. 195.

    Article  CAS  Google Scholar 

  27. Wood, P.L. and Khan, M.A., J. Chromatogr. B: Anal. Technol. Biomed. Life Sci., 2006, vol. 831, nos. 1–2, p. 313.

    Article  CAS  Google Scholar 

  28. Vinod, V.T.P., Sashidhar, R.B., Sarma, V.U.M., and Raju, S.S., Food Chem., 2010, vol. 123, no. 1, p. 57.

    Article  CAS  Google Scholar 

  29. Kouremenos, K.A., Harynuk, J.J., Winniford, W.L., Morrison, P.D., and Marriott, P.J., J. Chromatogr. B: Anal. Technol. Biomed. Life Sci., 2010, vol. 878, no. 21, p. 1761.

    Article  CAS  Google Scholar 

  30. Mandalakis, M., Apostolaki, M., and Stephanou, E.G., J. Chromatogr. A, 2010, vol. 1217, no. 1, p. 143.

    Article  CAS  Google Scholar 

  31. Long, G.L. and Winefordner, J.D., Anal. Chem., 1983, vol. 44, p. 712.

    Google Scholar 

  32. Jing, Y.Q., Zhang, B.L., Yuan, X.X., Gao, Y.Z., Lu, P., Wang, W.F., and Xu, M., Saudi J. Biol. Sci., 2016, vol. 23, no. 1, p. 64.

    Article  Google Scholar 

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Funding

This work was generously supported by the Research and Development Center of China Tobacco Sichuan Industrial Corporation which is hereby gratefully acknowledged.

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Authors and Affiliations

  1. Research and Development Center, China Tobacco Sichuan Industrial Corporation, 610066, Chengdu, China

    Jing Wang, Jun Long, De An Lei & Feng Gao

  2. Department of Chemistry, University of Science and Technology of China, 230023, Hefei, China

    Shao Min Liu

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  1. Jing Wang
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  2. Shao Min Liu
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  3. Jun Long
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Correspondence to Feng Gao.

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Jing Wang, Liu, S.M., Long, J. et al. Derivatization Method for the Determination of Amino Acids in Tobacco by Gas Chromatography–mass Spectrometry. J Anal Chem 75, 1046–1053 (2020). https://doi.org/10.1134/S1061934820080171

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  • DOI: https://doi.org/10.1134/S1061934820080171

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