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Study on mcl-PHA Production by Novel Thermotolerant Gram-Positive Isolate

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Abstract

The novel isolated thermotolerant bacteria Bacillus thermoamylovorans strain PHA005 was obtained from palm oil mill effluent. This strain showed an ability to produce medium-chain-length polyhydroxyalkanoates (mcl-PHAs) at 50.77% of dry cell weight (DCW) using sodium octanoate. Therefore, the production of mcl-PHA was enhanced by optimising the effects of nutrients and environmental conditions. The optimal conditions were 3.0 g/L sodium octanoate, a carbon to nitrogen ratio of 5:1 (0.02 g/L ammonium chloride), 45 °C incubation, initial pH 7.0 and agitation speed at 150 rpm. Under optimal conditions, the maximum mcl-PHA accumulation increased from 50.77% to 63.27% of DCW and 2.75 ± 0.03 g/L cell growth, 1.74 ± 0.02 g/L mcl-PHA concentration was achieved after 120 h cultivation. The composition of mcl-PHA contained C8-C18 while 3-hydroxyhexadecanoic (3HHD) was found at the highest composition (39.25 mol%) which has not been previously reported.

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Acknowledgements

The authors would like to thank Thailand Research Fund (TRF) Grant for Researcher (project number RSA 6180066), Energy Policy and Planning Office (EPPO), Ministry of Energy, Thailand and Development Institute at Thaksin University for financial support.

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Authors and Affiliations

  1. Department of Biology, Faculty of Science, Thaksin University, Phatthalung, 93210, Thailand

    Aophat Choonut

  2. Research and Development Office, Prince of Songkhla University, Songkhla, 90112, Thailand

    Poonsuk Prasertsan

  3. Department of Agro and Bio Industry, Thaksin University, Phatthalung, 93210, Thailand

    Sappasith Klomklao

  4. Department of Chemistry, Faculty of Science, Thaksin University, Phatthalung, 93210, Thailand

    Kanokphorn Sangkharak

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  1. Aophat Choonut
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  2. Poonsuk Prasertsan
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  3. Sappasith Klomklao
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  4. Kanokphorn Sangkharak
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Correspondence to Kanokphorn Sangkharak.

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Choonut, A., Prasertsan, P., Klomklao, S. et al. Study on mcl-PHA Production by Novel Thermotolerant Gram-Positive Isolate. J Polym Environ 28, 2410–2421 (2020). https://doi.org/10.1007/s10924-020-01779-8

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  • DOI: https://doi.org/10.1007/s10924-020-01779-8

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