Skip to main content

Advertisement

SpringerLink
Log in

Cost-effective production of dextran using Saccharum officinarum juice (SOJ) as a potential feedstock: downstream processing and characterization

  • Original Article
  • Published:
Biomass Conversion and Biorefinery Aims and scope Submit manuscript

Abstract

Dextrans are exo-polysaccharides prominently used as biomaterial on the grounds of their biodegradability and biocompatibility characteristics. There is an increasing interest and need to identify alternative cost-effective and renewable carbon source for dextran to reduce the production cost and to improve the overall economy of dextran production. In this work, dextran was produced using Saccharum officinarum juice (SOJ) as a low-carbon source by Leuconostoc mesenteroides MTCC 7337. The suitable condition/parameter for the production of dextran was found to be medium pH, 7; shaking speed, 150 rpm; inoculum size, 5% v/v; and nitrogen source, yeast extract. The different downstream factors including screening of solvent, supernatant to solvent ratio and precipitation time on the recovery of dextran, were studied. The organic solvent, ethanol with volume ratio of 1:4 (supernatant to solvent ratio), and precipitation time of 16 h were selected based on the maximum recovery of dextran from SOJ medium. The purified dextran was characterized by proton nuclear magnetic resonance spectroscopy (1H-NMR), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA) analysis. The results of rheological studies indicate that dextran solution behaves like a pseudoplastic fluid at higher concentrations of dextran solution. Based on the results, it was found that SOJ could be used as an alternate substrate for the production of dextran.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  1. Ponnusami V, Gunasekar V (2015) Production of pullulan by microbial fermentation. In: Polysaccharides: Bioactivity and Biotechnology, pp 1–2241

    Google Scholar 

  2. Li RH, Bin ZH, Hu XQ et al (2016) An efficiently sustainable dextran-based flocculant: synthesis, characterization and flocculation. Chemosphere 159:342–350

    Google Scholar 

  3. Capek P, Hlavoňová E, Matulová M et al (2011) Isolation and characterization of an extracellular glucan produced by Leuconostoc garlicum PR. Carbohydr Polym 83:88–93

    Google Scholar 

  4. Falconer DJ, Mukerjea R, Robyt JF (2011) Biosynthesis of dextrans with different molecular weights by selecting the concentration of B-512FMC dextransucrase, the sucrose concentration, and the temperature. Carbohydr Res 346:280–284

    Google Scholar 

  5. Naessens M, Cerdobbel A, Soetaert W, Vandamme EJ (2005) Leuconostoc dextransucrase and dextran: Production, properties and applications. J Chem Technol Biotechnol 80:845–860

    Google Scholar 

  6. Zhang Z, Liu Z, Tao X, Wei H (2016) Characterization and sulfated modification of an exopolysaccharide from Lactobacillus plantarum ZDY2013 and its biological activities. Carbohydr Polym 153:25–33

    Google Scholar 

  7. Purama RK, Goyal A (2008) Identification , effective purification and functional characterization of dextransucrase from Leuconostoc mesenteroides NRRL B-640. Bioresour Technol 99:3635–3642

    Google Scholar 

  8. Vettori MHPB, Blanco KC, Cortezi M et al (2012) Dextran: effect of process parameters on production, purification and molecular weight and recent applications. Diálogos & Ciência 2012:171–186

    Google Scholar 

  9. Qader SAU, Iqbal L, Aman A et al (2005) Production of dextran by newly isolated strains of Leuconostoc mesenteroides PCSIR-4 and PCSIR-9. Turkish J Biochem 31:21–26

    Google Scholar 

  10. Du R, Qiao X, Zhao F et al (2018) Purification, characterization and antioxidant activity of dextran produced by Leuconostoc pseudomesenteroides from homemade wine. Carbohydr Polym 198:529–536

    Google Scholar 

  11. Liu J, Wang X, Pu H et al (2017) Recent advances in endophytic exopolysaccharides: production, structural characterization, physiological role and biological activity. Carbohydr Polym 157:1113–1124

    Google Scholar 

  12. Du R, Xing H, Yang Y et al (2017) Optimization, purification and structural characterization of a dextran produced by L. mesenteroides isolated from Chinese sauerkraut. Carbohydr Polym 174:409–416

    Google Scholar 

  13. Brigham CJ, Kurosawa K, Rha C, Sinskey AJ (2011) Bacterial carbon storage to value added products. J Microb Biochem Technol:s3

  14. Agrawal M, Shukla R, Goyal A (2011) UV mutagenesis of Leuconostoc mesenteroides NRRL B-640 for generation of a mutant (B-640 M) with hyper-producing dextransucrase activity. Curr Trends Biotechnol Pharm 5:1445–1453

    Google Scholar 

  15. Moussa TAA, Khalil NM (2012) Solid-state fermentation for the production of dextran from Saccharomyces cerevisiae and its cytotoxic effects. Life Sci J 9:2210–2218

    Google Scholar 

  16. Moosavi-nasab M, Gavahian M, Yousefi AR et al (2010) Fermentative production of dextran using food industry wastes. World Acad Sci Eng Technol 44:1043–1045

    Google Scholar 

  17. Behravan J, Fazly Bazzaz BS, Salimi Z (2003) Optimization of dextran production by Leuconostoc mesenteroides NRRL B-512 using cheap and local sources of carbohydrate and nitrogen. Biotechnol Appl Biochem 38:267

    Google Scholar 

  18. Esmaeilnejad-Moghadam B, Mokarram RR, Hejazi MA et al (2019) Low molecular weight dextran production by Leuconostoc mesenteroides strains: optimization of a new culture medium and the rheological assessments. Bioact Carbohydrates Diet Fibre 18:100181

    Google Scholar 

  19. Han J, Hang F, Guo B et al (2014) Dextran synthesized by Leuconostoc mesenteroides BD1710 in tomato juice supplemented with sucrose. Carbohydr Polym 112:556–562

    Google Scholar 

  20. Onilude AA, Olaoye O, Fadahunsi IF et al (2013) Effects of cultural conditions on dextran production by Leuconostoc spp. Int Food Res J 20:1645–1651

    Google Scholar 

  21. Srichayet P, Limsangouan N, Puntapurt K (2000) The composition of sugarcane juice and production of granulated sugar. 2000

  22. Wei P, Cheng C, Lin M et al (2017) Production of poly (malic acid) from sugarcane juice in fermentation by Aureobasidium pullulans: kinetics and process economics. Bioresour Technol 224:581–589

    Google Scholar 

  23. Kayalvizhi V, Antony U (2011) Microbial a nd physico-chemical changes in tomato juice subjected to pulsed electric field treatment. African J Agric Res 6:6348–6353

    Google Scholar 

  24. Singh R, Gaur R, K. Pandey P, et al (2018) A novel media optimized for production of pullulan in flask type fermentation system. Int J Curr Microbiol Appl Sci 7:53–61.

  25. Tsuchiya H, Koepsell H, Corman J et al (1952) The effect of certain cultural factors on production of dextransucrase by Leuconostoc mesenteroides. North Reg Res Lab 64:521–526

    Google Scholar 

  26. Zarour K, Goretti M, Prieto A et al (2017) Rheology and bioactivity of high molecular weight dextrans synthesised by lactic acid bacteria. Carbohydr Polym 174:646–657

    Google Scholar 

  27. Rao MA, Cooley HJ, Vitali AA (1984) Flow properties of concentrated juices at low temperatures. Food Technol

  28. Farinazzo FS, Valente LJ, Almeida MB et al (2020) Characterization and antioxidant activity of an exopolysaccharide produced by Leuconostoc pseudomesenteroides JF17 from juçara fruits (Euterpe edulis Martius). Process Biochem 91:141–148

    Google Scholar 

  29. Choudhury AR, Sharma N, Prasad GS (2012) Deoiledjatropha seed cake is a useful nutrient for pullulan production. Microb Cell Fact 11:3–11

    Google Scholar 

  30. Lule VK, Singh R, Pophaly SD et al (2016) Production and structural characterisation of dextran from an indigenous strain of Leuconostoc mesenteroides BA08 in Whey. Int J Dairy Technol 69:520–531

    Google Scholar 

  31. Santos M, Teixeira J, Rodrigues A (2000) Production of dextransucrase, dextran and fructose from sucrose using Leuconostoc mesenteroides NRRL B512 ( f ). Biochem Eng J 4:177–188

    Google Scholar 

  32. Sarwat F, Qader SAU, Aman A, Ahmed N (2008) Production & characterization of a unique dextran from an indigenous Leuconostoc mesenteroides CMG713. Int J Biol Sci 4:379–386

    Google Scholar 

  33. Kanimozhi J, Moorthy IG, Sivashankar R, Sivasubramanian V (2017) Optimization of dextran production by Weissella cibaria NITCSK4 using response surface methodology-genetic algorithm based technology. Carbohydr Polym 174:103–110

    Google Scholar 

  34. Otts DR, Day DF (1988) Dextransucrase secretion in Leuconostoc mesenteroides depends on the presence of a transmembrane proton gradient. J Bacteriol 170:5006–5011

    Google Scholar 

  35. Lazić ML, Veljković VB, Vučetić JI, Vrvić MM (1993) Effect of pH and aeration on dextran production by Leuconostoc mesenteroides. Enzyme Microb Technol 15:334–338

    Google Scholar 

  36. Karthikeyan RS, Rakshit SK, Baradarajan A (1996) Optimization of batch fermentation conditions for dextran production. Bioprocess Eng 15:247–251

    Google Scholar 

  37. Vedyashkina TA, Revin VV, Gogotov IN (2005) Optimizing the conditions of dextran synthesis by the bacterium Leuconostoc mesenteroides grown in a molasses-containing medium. Appl Biochem Microbiol 41:409–413

    Google Scholar 

  38. Zhang ZY, Jin B, Kelly JM (2007) Production of lactic acid from renewable materials by Rhizopus fungi. Biochem Eng J 35:251–263

    Google Scholar 

  39. Srinivas B, Padma PN (2014) Screening Of Diverse Organic, Inorganic And Natural Nitrogen Sources For Dextran Production By Weissella Sps Using Plackett-Burman Design. 3:234–237

  40. Abedin RM, El-Borai AM, Shall MA, El-Assar SA (2013) Optimization and statistical evaluation of medium components affecting dextran and dextransucrase production by Lactobacillus acidophilus ST76480.01. Life Sci J 10:1746–1753

    Google Scholar 

  41. Shukla S, Goyal A (2011) Optimization of fermentation medium for enhanced glucansucrase and glucan production from Weissella confusa. Brazilian Arch Biol Technol 54:1117–1124

    Google Scholar 

  42. Baruah R, Deka B, Kashyap N, Goyal A (2018) Dextran utilization during its synthesis by Weissella cibaria RBA12 can be overcome by fed-batch fermentation in a bioreactor. Appl Biochem Biotechnol 184

  43. Majumder A, Bhandari S, Purama RK et al (2009) Enhanced production of a novel dextran from Leuconostoc mesenteroides NRRL B-640 by Response Surface Methodology. 59:309–315

  44. Fattah AFA, Hashem AM, El-refai MA, Gebreel HM (2012) Production and properties of dextransucrase by free and immobilized cells of Leuconostoc paramesenteroides. Egypt Pharm J:42–48

  45. Wang X, Xu P, Yuan Y et al (2006) Modeling for gellan gum production by. Microbiology 72:3367–3374

    Google Scholar 

  46. Hamieh A, Olama Z, Holail H (2013) Microbial production of polyhydroxybutyrate , a biodegradable plastic using agro-industrial waste. Glob Adv Res J Microbiol 2:54–64

    Google Scholar 

  47. Srinivas B, Padma PN (2015) Effect of process parameters on dextran production by Weissella Confusa. Int J Res Appl Sci Eng 3:373–377

    Google Scholar 

  48. Qader SAU, Aman A (2012) Low molecular weight dextran: Immobilization of cells of Leuconostoc mesenteroides KIBGE HA1 on calcium alginate beads. Carbohydr Polym 87:2589–2592

    Google Scholar 

  49. Devi N, Singh AK, Azmi W (2018) Analysis of the combined effects of aeration and agitation rate on dextransucrase production by Leuconostoc lactis KU665298 in a Laboratory Fermenter using Response Surface Methodology. J Microbiol Biotechnol 7:41–52

    Google Scholar 

  50. Baruah R, Deka B, Kashyap N (2017) Dextran utilization during its synthesis by Weissella cibaria RBA12 can be overcome by fed-batch fermentation in a bioreactor. Appl Biochem Biotechnol 184:1–11

    Google Scholar 

  51. Heilig ML (1994) United States Patent Office. ACM SIGGRAPH Comput Graph 28:131–134

    Google Scholar 

  52. Feng F, Zhou Q, Yang Y et al (2018) Characterization of highly branched dextran produced by Leuconostoc citreum B-2 from pineapple fermented product. Int J Biol Macromol 113:45–50

    Google Scholar 

  53. Robyt JF, Kimble BK, Walseth TF (1974) The mechanism of dextransucrase action. Direction of dextran biosynthesis. Arch Biochem Biophys 165:634–640

    Google Scholar 

  54. Youssef F, Roukas T, Biliaderis CG (1999) Pullulan production by a non-pigmented strain of Aureobasidium pullulans using batch and fed-batch culture. Process Biochem 34:355–366

    Google Scholar 

  55. Sugumaran KR, Ponnusami V (2017) Conventional optimization of aqueous extraction of pullulan in solid-state fermentation of Cassava bagasse and Asian palm kernel. Biocatal Agric Biotechnol 10:204–208

    Google Scholar 

  56. Huang J, Zhu S, Li C, et al (2019) Cost-effective optimization of gellan gum production by Sphingomonas paucimobilis using corn steep liquor. Prep Biochem Biotechnol 0:1–7.

  57. Prechtl RM, Wefers D, Jakob F, Vogel RF (2018) Cold and salt stress modulate amount, molecular and macromolecular structure of a Lactobacillus sakei dextran. Food Hydrocoll 82:73–81

    Google Scholar 

  58. Landon RS, Law RCS, Webb C (1993) Fermentation broth rheology during dextran production by Leuconostoc mesenteroides B512 ( F ) as a possible tool for control. Appl Microbiol Biotechnol 40:251–257

    Google Scholar 

  59. Tirtaatmadja V, Dunstan DE, Boger DV (2001) Rheology of dextran solutions. J Nonnewton Fluid Mech 97:295–301

    Google Scholar 

  60. Prasanna PHP, Bell A, Grandison AS, Charalampopoulos D (2012) Emulsifying, rheological and physicochemical properties of exopolysaccharide produced by Bifidobacterium longum subsp. infantis CCUG 52486 and Bifidobacterium infantis NCIMB 702205. Carbohydr Polym 90:533–540

    Google Scholar 

  61. Santos M, Rodrigues A, Teixeira JA (2005) Production of dextran and fructose from carob pod extract and cheese whey by Leuconostoc mesenteroides NRRL B512 ( f ). Biochem Eng J 25:1–6

    Google Scholar 

  62. Moosavi-nasab M, Alahdad Z, Nazemi S (2009) Characterization of the dextran produced by Leuconostoc mesenteroides from date fruit extract. Iran Agric Res 27:79–88

    Google Scholar 

  63. Mothé CG, Rao MA (1999) Rheological behavior of aqueous dispersions of cashew gum and gum arabic: effect of concentration and blending. Food Hydrocoll 13:501–506

    Google Scholar 

  64. Wang ZM, Cheung YC, Leung PH, Wu JY (2010) Ultrasonic treatment for improved solution properties of a high-molecular weight exopolysaccharide produced by a medicinal fungus. Bioresour Technol 101:5517–5522

    Google Scholar 

  65. Zhu KX, Huang S, Peng W et al (2010) Effect of ultrafine grinding on hydration and antioxidant properties of wheat bran dietary fiber. Food Res Int 43:943–948

    Google Scholar 

  66. Kavitake D, Devi PB, Singh SP, Shetty PH (2016) Characterization of a novel galactan produced by Weissella confusa KR780676 from an acidic fermented food. Int J Biol Macromol 86:681–689

    Google Scholar 

  67. Zhou Q, Feng F, Yang Y et al (2017) Characterization of a dextran produced by Leuconostoc pseudomesenteroides XG5 from homemade wine. Int J Biol Macromol:1–8

  68. Bertocchi C, Delneri D, Signore S et al (1997) Characterization of microbial cellulose from a high-producing mutagenized Acetobacter pasteurianus strain. Biochim Biophys Acta - Gen Subj 1336:211–217

    Google Scholar 

  69. Purama RK, Goswami P, Khan AT, Goyal A (2009) Structural analysis and properties of dextran produced by Leuconostoc mesenteroides NRRL B-640. Carbohydr Polym 76:30–35

    Google Scholar 

  70. Yang Y, Peng Q, Guo Y et al (2015) Isolation and characterization of dextran produced by Leuconostoc citreum NM105 from manchurian sauerkraut. Carbohydr Polym 133:365–372

    Google Scholar 

  71. Rosca I, Roxana A, Peptanariu D et al (2017) Biosynthesis of dextran by Weissella confusa and its In vitro functional characteristics. Int J Biol Macromol.

  72. Zamora F, González MC, Dueñas MT et al (2002) Thermodegradation and thermal transitions of an exopolysaccharide produced by Pediococcus damnosus 2.6. J Macromol Sci - Phys 41 B:473–486

    Google Scholar 

  73. Kenari HS, Imani M, Nodehi A (2013) Full factorial design-of-experiments for preparation of crosslinked dextran microspheres. J Appl Polym Sci 127:3712–3724

    Google Scholar 

  74. Miao M, Huang C, Jia X et al (2015) Physicochemical characteristics of a high molecular weight bioengineered α-D-glucan from Leuconostoc citreum SK24.002. Food Hydrocoll 50:37–43

    Google Scholar 

  75. Llamas-arriba MG, Puertas AI, Prieto A et al (2019) Characterization of dextrans produced by Lactobacillus mali CUPV271 and Leuconostoc carnosum CUPV411 María. Food Hydrocoll 89:613–622

    Google Scholar 

  76. Sun YX, Liu JC, Yang XD, Kennedy JF (2010) Purification, structural analysis and hydroxyl radical-scavenging capacity of a polysaccharide from the fruiting bodies of Russula virescens. Process Biochem 45:874–879

    Google Scholar 

Download references

Acknowledgments

All authors are thankful to the Management SASTRA Deemed to be University, Thanjavur, Tamil Nadu, India, for providing the necessary facilities. Ms. Sameeha Syed Abdul Rahman, who is a research scholar, is a recipient of the DST – INSPIRE fellowship (IF180367) from the Department of Science and Technology, Government of India, and the support is duly acknowledged.

Author information

Authors and Affiliations

  1. Bioprocess Engineering Laboratory, Centre for Bioenergy, School of Chemical & Biotechnology, SASTRA Deemed to be University, Thanjavur, Tamil Nadu, 613401, India

    Sameeha Syed Abdul Rahman, Ponnusami Venkatachalam & Sugumaran Karuppiah

Authors
  1. Sameeha Syed Abdul Rahman
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ponnusami Venkatachalam
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sugumaran Karuppiah
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sugumaran Karuppiah.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

ESM 1

(DOCX 33 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Rahman, S.S.A., Venkatachalam, P. & Karuppiah, S. Cost-effective production of dextran using Saccharum officinarum juice (SOJ) as a potential feedstock: downstream processing and characterization. Biomass Conv. Bioref. 12, 4863–4875 (2022). https://doi.org/10.1007/s13399-020-00926-4

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13399-020-00926-4

Keywords

Search

Navigation