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QTLs detection and pyramiding for stigma exsertion rate in wild rice species by using the single-segment substitution lines

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Abstract

Seed yield is one of the main limitations in hybrid rice production. The improvement of stigma exsertion rate (SER) of male sterility lines is essential for the higher seed yield in hybrid rice. Wild rice species are valuable germplasms with exserted stigma. It is worthy of mining-related genes for SER in wild rice species, as well as probing the way to use the beneficial genes in rice breeding. In the present study, we identified a total number of 36 stable QTLs for the SER distributing on 11 chromosomes by using a set of single-segment substitution lines (SSSLs) of 3 AA-genome wild rice species, O. barthii, O. meridionalis, and O. rufipogon. The mean genetic effects of these QTLs were ranged from 14.50 to 41.25% during 2016–2017. Twelve QTLs among them were novel by comparing the chromosome intervals with the reported QTLs. Besides, 7 homozygous 2-QTL pyramiding lines (PLs) were developed in BAR-SSSLs and MER-SSSLs to detect QTL interactions on the same background of HJX74. The epistatic effects in 3 out of 7 QTL pairs were positive, including qSERb3-1/qSERb6-1, qSERb3-1/qSERb8-1, and qSERb3-1/qSERb12-1, which mean epistatic effect was 13.56%, 4.76%, and 7.29% in 2018–2019, respectively. The PL of B6/B19 (qSERb3-1/qSERb12-1) had the highest mean SER of 80.59% and the largest pyramiding effect of 55.51%. The results in the present study demonstrated that SSSLs were the elite materials for SER QTL detection from wild rice species, and elite PLs could be developed among SSSLs for SER improvement in hybrid rice breeding.

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Abbreviations

BAR :

O. barthii

DES:

Dual exserted stigma

e :

Epistatic effect

MAS:

Marker-assisted selection

MER :

O. meridionalis

NES:

No exserted stigma

p :

Pyramiding effect

PL:

Pyramiding line

QTL:

Quantitative trait loci

RUF :

O. rufipogon

SER:

Stigma exsertion rate

SES:

Single exserted stigma

SSR:

Simple sequence repeats

SSSLs:

Single-segment substitution lines.

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Acknowledgments

Our thanks are given to Professor Guiquan Zhang for the valuable discussions on the research. Our thanks also go to Senior agronomist Haitao Zhu for the management of the field experiments and Dr. Ping He for his help during the processes of writing and revising the manuscript. And we appreciate Ms. Humera Ashraf and Mr. Zi Ran for their critical reading of this manuscript and English grammar corrections.

Funding

This work was supported by the National Natural Science Foundation of China (31671762) and the Guangzhou Science and Technology Program Key Project (201804020086).

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Author notes

  1. Tuo Zou

    Present address: Institute of Coastal Agriculture, Hebei Academy of Agriculture and Forestry Sciences, Tangshan, 063299, China

  2. Mingmin Zheng

    Present address: Drug Research Center, Traditional Chinese Medicine Institute of Jiangxi, Nanchang, 330046, China

  3. Tuo Zou and Hanwei Zhao contributed equally to this work.

Authors and Affiliations

  1. Guangdong Provincial Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China

    Tuo Zou, Hanwei Zhao, Xiaohui Li, Mingmin Zheng, Shaodi Zhang, Lingling Sun, Ning He, Xiaoping Pan, Ziqiang Liu & Xuelin Fu

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  1. Tuo Zou
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Supplementary Fig. 1

SER distributions in SSSLs (a) and pyramiding lines (b). “*”, “**”, and “***” indicate the significant differences at P levels of 0.05, 0.01, and 0.001, respectively, by comparing the mean SER in SSSLs and PLs with HJX74. (JPG 2577 kb)

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Zou, T., Zhao, H., Li, X. et al. QTLs detection and pyramiding for stigma exsertion rate in wild rice species by using the single-segment substitution lines. Mol Breeding 40, 74 (2020). https://doi.org/10.1007/s11032-020-01157-1

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