Abstract
Halotolerant species are adapted to dealing continually with hyperosmotic environments, having evolved strategies that are uncommon in other organisms. The HOG pathway is the master system that regulates the cellular adaptation under these conditions; nevertheless, apart from the importance of Debaryomyces hansenii as an organism representative of the halotolerant class, its HOG1 pathway has been poorly studied, due to the difficulty of applying conventional recombinant DNA technology. Here we describe for the first time the phenotypic characterisation of a null HOG1 mutant of D. hansenii. Dhhog1Δ strain was found moderately resistant to 1 M NaCl and sensitive to higher concentrations. Under hyperosmotic shock, DhHog1 fully upregulated transcription of DhSTL1 and partially upregulated that of DhGPD1. High osmotic stress lead to long-term inner glycerol accumulation that was partially dependent on DhHog1. These observations indicated that the HOG pathway is required for survival under high external osmolarity but dispensable under low and mid-osmotic conditions. It was also found that DhHog1 can regulate response to alkali stress during hyperosmotic conditions and that it plays a role in oxidative and endoplasmic reticulum stress. Taken together, these results provide new insight into the contribution of this MAPK in halotolerance of this yeast.
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Acknowledgements
NSS is very grateful to Dr Juan Pablo Pardo, of the UNAM school of Medicine, for fruitful discussions throughout the project. The authors wish to thank Dr Yazmín Ramiro for her help with the Two-Photon Confocal Microscopy. We are also grateful to Dr Laura Ongay, MSc Minerva Mora and Biol Guadalupe Códiz from the Molecular Biology Unit. We would like to recognise Dr Laura Kawasaki and Francisco Padilla for their technical assistance. Dr Mariana Hernández-Elvira helped NSS with some methodology. We are also grateful to Gerardo Coello, Ana María Escalante, Juan Barbosa and Ivette Rosas from the Computing Unit and Manuel Ortínez and Aurey Galván from the maintenance workshop. NSS is a PhD student in the Doctorate Program in Biochemical Science, UNAM. This work was supported by CONACyT (Grant No. CB-238497 to AP) and PAPIIT, DGAPA, UNAM (Grants IN223399 and IN202103 to AP and IN210519 to RC). We also extend our thanks to Patrick Weill for revising the English grammar of the text.
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Sánchez, N.S., Calahorra, M., González, J. et al. Contribution of the mitogen-activated protein kinase Hog1 to the halotolerance of the marine yeast Debaryomyces hansenii. Curr Genet 66, 1135–1153 (2020). https://doi.org/10.1007/s00294-020-01099-3
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Issue Date:
DOI: https://doi.org/10.1007/s00294-020-01099-3