Abstract
Nectaroscordum siculum ssp. bulgaricum (Janka) Stearn (Allium siculum subsp. dioscoridis (Sm.) K. Richt.) is a traditional culinary spice from South-East Europe. Studies of N. siculum have focused mainly on the botanical and taxonomic characteristics of this species and there is no data available in the scientific literature about its metabolite profile. Thus, the aim of the current study was metabolite profiling of four wild populations of N. siculum grown in Bulgaria by gas chromatography coupled to mass spectrometry (GC-MS) and subsequent principal component analysis (PCA) of the data obtained. The identified primary metabolites (carbohydrates, amino acids, organic acids and lipids) are initial compounds for the biosynthesis of different plant secondary metabolites, such as polyphenols and flavour compounds with valuable biological activities for humans. The health benefits of the phenolic acids identified in this study have been a prerequisite for the implementation of N. siculum in different food systems in order to increase their quality and biological value.
Funding source: Bulgarian Science Fund, Bulgarian Ministry of Education and Science
Acknowledgements
This work was supported by the Bulgarian Science Fund, Bulgarian Ministry of Education and Science by contract ДM 06/1 – 13.12.2016.
Author contribution: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: This work was supported by the Bulgarian Science Fund, Bulgarian Ministry of Education and Science by contract ДM 06/1 – 13.12.2016.
Conflict of interest statement: The authors report no declarations of interest.
References
1. Chives, CH. In: Peter, KV, editor. Handbook of herbs and spices. USA: CRC Press LLC; 2006:337 p, vol. 3.10.1533/9781845691717.3.337Search in Google Scholar
2. Govaerts, RHA. World checklist of selected plant families published update. Facilitated by the trustees of the royal botanic gardens; 2011. Kew.Search in Google Scholar
3. Delipavlov, D, Popova, M, Kovachev, I, Terziyski, D, Cheshmedzhiev, I, Gramatikov, D. Key to the plants in Bulgaria. Bulgaria: Zemizdat press; 1992, in Bulgarian.Search in Google Scholar
4. Radanova, S. Floristic analysis of the plant cover in the Northern part of the forest shelter belt of Stara Zagora. J For Sci 2006;61:43–52.Search in Google Scholar
5. Alexieva, I, Mihaylova, D, Popova, A. Evaluation of the antioxidant capacity of aqueous extracts of fresh samardala (Allium bulgaricum L.) leaves. Sci Works UFT 2013;60:826–831.Search in Google Scholar
6. Cevallos-Cevallos, JM, Futch, DB, Shilts, T, Folimonova, SY, Reyes-De-Corcuera, JI. GC-MS metabolomics differentiation of selected citrus varieties with different sensitivity to citrus huanglongbing. Plant Physiol Biochem 2012;53:69–76. https://doi.org/10.1016/j.plaphy.2012.01.010.Search in Google Scholar
7. Keutgen, AJ, Pawelzik, E. Quality and nutritional value of strawberry fruit under long term salt stress. Food Chem 2008;107:1413–20. https://doi.org/10.1016/j.foodchem.2007.09.071.Search in Google Scholar
8. Kopka, J. Current challenges and developments in GC-MS based metabolite profiling technology. J Biotechnol 2006;124:312–22. https://doi.org/10.1016/j.jbiotec.2005.12.012.Search in Google Scholar
9. Torras-Claveria, L, Berkov, S, Jauregui, O, Caujape, J, Viladomat, F, Codina, C, et al. Metabolic profiling of bioactive Pancratium canariense extracts by GC-MS. Phytochem Anal 2010;21:80–8. https://doi.org/10.1002/pca.1158.Search in Google Scholar
10. Berkov, S, Georgieva, L, Sidjimova, B, Nikolova, M. Metaboli te profiling of in vitro plant systems. In: Pavlov, A, Bley, T, editors. Bioprocessing of plant in vitro systems. Reference series in phytochemistry. Switzerland: Springer International Publishing AG; 2018:67 p.10.1007/978-3-319-54600-1_12Search in Google Scholar
11. Berkov, S, Bastida, J, Viladomat, F, Codina, C. Development and validation of a GC-MS method for rapid determination of galantamine in Leucojum aestivum and Narcissus spp.: A metabolic approach. Talanta 2011;83:1455–65. https://doi.org/10.1016/j.talanta.2010.11.029.Search in Google Scholar
12. Wagner, C, Sefkow, M, Kopka, J. Construction and application of mass spectral and retention time index database generated from plant GC/EI-TOF-MS metabolite profiles. Phytochemistry 2003;62:887–900. https://doi.org/10.1016/s0031-9422(02)00703-3.Search in Google Scholar
13. Kwon, Y-K, Bong, YS, Lee, KS, Hwang, GS. An integrated analysis for determining the geographical origin of medicinal herbs using ICP-AES/ICP-MS and 1H NMR analysis. Food Chem 2014;161:168–75. https://doi.org/10.1016/j.foodchem.2014.03.124.Search in Google Scholar PubMed
14. Lee, D –K, Yoon, MH, Kang, YP, Yu, J, Park, JH, Lee, J, et al. Comparison of primary and secondary metabolites for suitability to discriminate the origins of Schisandra chinensis by GC/MS and LC/MS. Food Chem 2013;141:3931–7. https://doi.org/10.1016/j.foodchem.2013.06.064.Search in Google Scholar PubMed
15. Arcus, M, Popescu, A, Doroftei, E, Sava, D Pharmacognostic comparative study of the vegetal products Nectaroscordii bulbus and Nectaroscordii herba. Ann Rom Soc Cell Biol 2009;14:266–270.Search in Google Scholar
16. Friesen, N, Fritsch, RM, Pollner, S, Blattner, FR. Molecular and morphological evidence for an origin of the aberrant genus Milula within Himalayan species of Allium (Alliaceae). Mol Phylogenet Evol 2000;17:209–18. https://doi.org/10.1006/mpev.2000.0844.Search in Google Scholar
17. Friesen, N, Fritsch, RM, Blattner, FR. Phylogeny and new intrageneric classification of Allium (Alliaceae) based on nuclear ribosomal DNA ITS sequences. Aliso 2006;22:372–95. https://doi.org/10.5642/aliso.20062201.31.Search in Google Scholar
18. Hardalova, R, Evstatieva, L, Gussev, C. Characterization of wild medicinal plant resources in Bulgaria and recommendations for their longterm stable development. In Sakalian, M, editor. National strategy for biological diversity protection. Sofia: Bulvest press; 1994:41 p. (in Bulgarian).Search in Google Scholar
19. Krzymiñska, A, Gawłowska, M, Wolko, B, Bocianowski, J. Genetic diversity of ornamental Allium species and cultivars assessed with isozymes. J Appl Genet 2008;49:213–20. https://doi.org/10.1007/bf03195616.Search in Google Scholar
20. Kürkcüoglu, M, Husnu Can Baser, K. Headspace volatiles of three Turkish plants. J Essent Oil Res 2010;22:389–92. https://doi.org/10.1080/10412905.2010.9700353.Search in Google Scholar
21. Kubec, R, Kim, S, Mckeon, DM, Musah, RA. Isolation of S-n butylcysteine sulfoxide and six n-butyl-containing thiosulfinates from Allium siculum. J Nat Prod 2020;65:960–4. https://doi.org/10.1021/np020064i.Search in Google Scholar
22. MetaboAnalyst - statistical, functional and integrative analysis of metabolomics data. MetaboAnalyst; 2019. https://www.metaboanalyst.ca/MetaboAnalyst/ModuleView.xhtml.Search in Google Scholar
23. Bourgaud, F, Gravot, A, Milesi, S, Gontier, E. Production of plant secondary metabolites: a historical perspective. Plant Sci 2001;161:839–51. https://doi.org/10.1016/s0168-9452(01)00490-3.Search in Google Scholar
24. Jones, MG, Hughes, J, Tregova, A, Milne, J, Tomsett, BA, Collin, HA. Biosynthesis of the flavour precursors of onion and garlic. J Exp Bot 2004;55:1903–18. https://doi.org/10.1093/jxb/erh138.Search in Google Scholar PubMed
25. Croteau, R, Kutchan, TM, Lewis, NG. Natural products (Secondary metabolites). In: Buchanan, B, Griussem, W, Jones, R, editors. Biochemistry and molecular biology of plants. Ames, Iowa: American Society of Plant Physiologists; 2000:1250 p.Search in Google Scholar
26. Akhatou, I, González-Domínguez, R. Fernández-Recamales, Á. Investigation of the effect of genotype and agronomic conditions on metabolomics profiles of selected strawberry cultivars with different sensitivity to environmental stress. Plant Physiol Biochem 2006;101:14–22. https://doi.org/10.1016/j.plaphy.2016.01.016.Search in Google Scholar PubMed
27. Yoshioka, T, Inokuchi, T, Fujioka, S, Kimura, Y. Phenolic compounds and flavonoids as plant growth regulators from fruit and leaf of Vitex rotundifolia. Z Naturforsch C 2004;59:509–14. https://doi.org/10.1515/znc-2004-7-810.Search in Google Scholar PubMed
28. Saxena, M, Saxena, J, Pradhan, A Flavonoids and phenolic acids as antioxidants in plants and human health. Int J Pharm Sci Rev Res 2012;16:130–134.Search in Google Scholar
29. Vrancheva, R, Stoyanova, M, Mihaylova, DS, Aneva, I, Deseva, I, Petkova, N, et al. Polyphenol profile and antioxidant activity of wild growing populations of Nectaroscordum siculum ssp. bulgaricum (Janka) Stearn in Bulgaria. Int Food Res J 2019;26:1635–40.Search in Google Scholar
© 2020 Walter de Gruyter GmbH, Berlin/Boston