Abstract
The enzymatic hydrolysis of residual cellulose casings from sausage processing was assessed. A central composite design (CCD) was performed to determine the best enzyme and substrate concentrations to release reducing sugars (RS) using the enzymatic complex Celluclast. The optimal response found in the CCD was a substrate concentration of 34.1 mg/mL and enzyme concentration of 1:200 (v:v). The combination two enzymatic preparations, Celluclast and Viscozyme, was evaluated to increase the final concentration of RS. The mixture of Celluclast (75%) and Viscozyme (25%) improved the reaction conversion and the glucose concentration. A substrate conversion of 85% in RS (31 mg/mL) was reached at 25 h, composed of 21 mg/mL of glucose and 10 mg/mL of cellobiose. The reaction was scaled-up 16-fold (from 0.25 to 4 L), and the enzymatic conversion remained constant. Thus, the results found in this work proved to be a good alternative for reusing this industrial waste.
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Abbreviations
- RS:
-
Reducing sugars
- CBHs:
-
Cellobiohydrolases
- EGs:
-
Endoglucanases
- CCD:
-
Central composite design
- CC:
-
Cellulose casings
- RCC:
-
Residual cellulose casings
- RCCW:
-
Washed residual cellulose casings
- FP:
-
Filter paper
- DNS:
-
3,5-Dinitrosalicylic acid
- DP:
-
Polymerization degree
- Ki :
-
Enzyme constant of product inhibition
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Acknowledgments
This work was supported by grants and scholarships from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), FAPERGS (process 17/2551-0000939-8) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001. We also thank the LNF Latino Americana for supplying the enzymes used in this work.
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Gabiatti Junior, C., Dal Magro, L., Graebin, N.G. et al. Combination of Celluclast and Viscozyme improves enzymatic hydrolysis of residual cellulose casings: process optimization and scale-up. Braz. J. Chem. Eng. 37, 463–473 (2020). https://doi.org/10.1007/s43153-020-00050-w
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DOI: https://doi.org/10.1007/s43153-020-00050-w