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Identification of metabolites present in Opuntia callus and study of their antioxidant, anti-inflammatory and anti-adipogenic properties

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Abstract

The metabolites present in Opuntia species have been widely studied by their health-promoting effects, but there is no information about the compounds present in Opuntia callus or their biological effects. The aim of this study was to identify the metabolites in Opuntia ficus indica, Opuntia megacantha and Opuntia streptacantha callus ethanolic extracts, and to analyze their antioxidant, anti-inflammatory and anti-adipogenic properties in mammalian cells. The compounds identified by chromatographic analysis and mass spectroscopy showed that the Opuntia callus from the three species synthesized 3,5-Di-O-galloylshikimic acid as major compound, as well as gallic acid and epicathequin derivates in lower concentration. The calluses metabolites reduced the formation of reactive oxygen species in Caco-2 cells similarly to the flavonoid isorhamnetin. The anti-inflammatory activity, measured as the production of nitric oxide, was inhibited between 20 and 68% in a murine macrophage cell line. This inhibition was higher with O. streptacantha callus extracts, followed by those of O. megacantha and O. ficus-indica. Additionally, a reduction on the lipid accumulation in 3T3 cells was observed, during and after adipocyte differentiation using extracts from the three Opuntia species. Our results prove that Opuntia calluses are a source of compounds with pharmacological relevance.

Key message

Opuntia calluses produce metabolites of pharmacological relevance.

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Acknowledgements

The authors thank to CONACYT for the scholarship granted to NGCR (365990), and to ITESM and FEMSA for their invaluable technical support.

Funding

This study was supported by “Fondo de Apoyo a la Investigación de la Universidad Autónoma de San Luis Potosí” (Grant Number 684).

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Authors

Contributions

NGCR conducted experiments and analyzed results. MAR and JGU coordinate the studies focused on the metabolites chemical identification, and antioxidant and anti-inflammatory studies. AJVC and APBR were in charge of anti-adipogenesis analysis; MSSD conceived, designed, and wrote the manuscript. All authors read and approved the manuscript.

Corresponding author

Correspondence to María del Socorro Santos-Díaz.

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This article does not contain any studies with human participants or animals performed by any of the authors.

Conflict of interest

Nancy Gabriela Camarena-Rangel, Marilena Antunes-Ricardo, Janet Gutiérrez-Uribe, Aida Jimena Velarde-Salcedo, Ana Paulina Barba-De la Rosa, and María del Socorro Santos-Díaz declare that there are no conflicts of interest.

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NGCR, MAR, JGU, AJVS, APBR and MSSD give their consent to participate in this paper.

Additional information

Communicated by Sergio J. Ochatt.

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Camarena-Rangel, N.G., Antunes-Ricardo, M., Gutiérrez-Uribe, J. et al. Identification of metabolites present in Opuntia callus and study of their antioxidant, anti-inflammatory and anti-adipogenic properties. Plant Cell Tiss Organ Cult 143, 31–43 (2020). https://doi.org/10.1007/s11240-020-01893-4

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  • DOI: https://doi.org/10.1007/s11240-020-01893-4

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