Abstract
Studies of insect feeding behavior are useful in different areas of entomology such as plant resistance, biology, and insecticide efficacy. For chewing insects, this kind of technique is well established, but for sap-sucking insects, especially tiny ones such as whiteflies, aphids, and psyllids, these tests can be laborious. Manipulation is difficult and can damage the plant, affecting the results. We describe here three types of cages for tests with small insects, one for seedlings in pots, a second for larger plants or plants in the field, and a third for caging insects on part of a leaf. These cages have been useful for different types of studies in addition to feeding behavior and can facilitate research with small phytophagous sucking insects.
References
Abràmoff MD, Magalhães PJ, Ram SJ (2004) Image processing with imageJ. Biophoton Int 11:36–41. https://doi.org/10.1201/9781420005615.ax4
Alves GR, Beloti VH, Faggioni-Floriano KM, Carvalho SM, Moral RA, Demétrio CCB, Parra JRP, Yamamoto PT (2018) Does the scion or rootstock of Citrus sp. affect the feeding and biology of Diaphorina citri Kuwayama (Hemiptera: Liviidae)? Arthropod Plant Interact 12:77–84. https://doi.org/10.1007/s11829-017-9555-z
Ammar ED, Hall DG (2011) A new method for short-term rearing of citrus psyllids (Hemiptera: Pysllidae) and for collecting their honeydew excretions. Florida Entomol 94:340–342. https://doi.org/10.1653/024.094.0229
Auclair JL (1958) Honeydew excretion in the pea aphid, Acyrthosiphon pisum (Harr.) (Homoptera: Aphididae). J Insect Physiol 2:330–337. https://doi.org/10.1016/0022-1910(58)90018-0
Baldin E, Vendramim J, Lourenção A (2019) Resistência de Plantas a Insetos: Fundamentos e Aplicações. FEALQ, Piracicaba, p 493
Boina DR, Onagbola EO, Salyani M, Stelinski LL (2009) Antifeedant and sublethal effects of imidacloprid on Asian citrus psyllid, Diaphorina citri. Pest Manag Sci 65:870–877. https://doi.org/10.1002/ps.1767
Chen XD, Ashfaq M, Stelinski LL (2018) Susceptibility of Asian citrus psyllid, Diaphorina citri (Hemiptera: Liviidae), to the insecticide afidopyropen: a new and potent modulator of insect transient receptor potential channels. Appl Entomol Zool 53:453–461. https://doi.org/10.1007/s13355-018-0574-8
Druege U, Franken P, Hajirezaei MR (2016) Plant hormone homeostasis, signaling, and function during adventitious root formation in cuttings. Front Plant Sci 7:381. https://doi.org/10.3389/fpls.2016.00381
Friedman M (2004) Applications of the ninhydrin reaction for analysis of amino acids, peptides, and proteins to agricultural and biomedical sciences. J Agric Food Chem 52:385–406. https://doi.org/10.1021/jf030490p
Jorge LAC, da Silva DJCB (2009) AFSoft: manual de utilização. Embrapa Instrumentação Agropecuária, São Carlos, 20p
Maluta N, Fereres A, Lopes JRS (2019) Plant-mediated indirect effects of two viruses with different transmission modes on Bemisia tabaci feeding behavior and fitness. J Pest Sci 92:405–416. https://doi.org/10.1007/s10340-018-1039-0
Parra JRP, Panizzi AR, Haddad ML (2012) Nutritional indices for measuring insect food intake and utilization. In: Panizzi AR, Parra JRP (eds) Insect bioecology and nutrition for integrated Pest management. CRC Press, Boca Raton, pp 13–51
Pogetto LD (2018) Potencial hospedeiro de duas espécies de Murraya para Diaphorina citri (Kuwayama , 1908) (Hemiptera: Liviidae) com vistas à produção de Tamarixia radiata Waterston , 1922 (Hymenoptera: Eulophidae). Universidade de São Paulo, p. 34. doi: https://doi.org/10.11606/D.11.2019.tde-13032019-144612
Silveira C (2019) Eficácia de inseticidas no controle de Dalbulus maidis (DeLong & Wolcott) (Hemiptera: Cicadellidae) e da transmissão de espiroplasma do milho. Universidade de São Paulo, p.77. doi: https://doi.org/10.11606/D.11.2020.tde-20012020-162602
Smith C, Khan Z, Pathak M (1993) Techniques for evaluating insect resistance in crop plants. CRC Press, Boca Raton, p 336
Tjallingii WF (1978) Eletronic recording of penetration behaviour by aphids. Entomol Exp Appl 24:721–730. https://doi.org/10.1111/j.1570-7458.1978.tb02836.x
Vale F, Fernandes-Filho E, Liberato J, Zambolin L (2001) Quant - a software to quantify plant disease severity. In: International workshop on plant disease epidemology. The International Society of Plant Pathology, Ouro Preto, p 160
Walquil J, Lopes J, Martins J, Viana PA, Maluta NKP (2019) Técnicas para Avaliação de Resistência. In: Baldin E, Vendramim J, Lourenção A (eds) Resistência de Plantas a Insetos: Fundamentos e Aplicações. FEALQ, Piracicaba, pp 255–313
Acknowledgments
We extend our thanks to Janet W. Reid (JWR Associates) for English revision, to the São Paulo Research Foundation for grants (FAPESP process numbers 2016/24998-9, 2013/04291-0 and SPARCBio - São Paulo Advanced Research Center for Biological Control, FAPESP process number 2018/02317-5), and to Sapiens Scientific Illustration for the figure.
Author information
Authors and Affiliations
Contributions
Gustavo Rodrigues Alves, Jaci Mendes Vieira, and Camila Haddad Silveira conceived the ideas for the cages, Gustavo Rodrigues Alves wrote the manuscript, and Alexandre José Ferreira Diniz and José Roberto Postali Parra wrote and revised the manuscript.
Corresponding author
Additional information
Edited by Gabriel Manrique – Univ de Buenos Aires
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Alves, G.R., Diniz, A.J.F., Vieira, J.M. et al. Practical and Simple Systems for Food Intake Studies on Phloem-Sucking Insects. Neotrop Entomol 50, 156–160 (2021). https://doi.org/10.1007/s13744-020-00803-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13744-020-00803-9