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Description of Erythrobacter mangrovi sp. nov., an aerobic bacterium from rhizosphere soil of mangrove plant (Kandelia candel)

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Abstract

A novel Gram-stain negative, aerobic, non-motile, rod-shaped bacterium, designated as strain EB310T, was isolated from rhizosphere soil of mangrove plant Kandelia candel in Fugong village, Zhangzhou, China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain EB310T belonged to the genus Erythrobacter, clustering with Erythrobacter pelagi JCM 17468T, Erythrobacter lutimaris KCTC 42109T and Erythrobacter marisflavi KCTC 62896T, and showed the highest 16S rRNA gene sequence similarity of 97.5% to Erythrobacter pelagi JCM 17468T. The genomic average nucleotide identity and in silico DNA–DNA hybridization values between strain EB310T and the reference strains were 71.0–75.5% and 19.8–20.0%, respectively. Growth ranges of the isolate occurred at 10–45 °C (optimum 28–30 °C), pH 5.5–9.5 (optimum pH 7.5) and 0–9.0% NaCl concentrations (optimum 2.0%, w/v). The strain did not produce bacteriochlorophyll a and flexirubin, but produced carotenoids. The strain contained Q-10 as the predominant ubiquinone and summed feature 3 (C16:1 ω7c/C16:1 ω6c) and summed feature 8 (C18:1 ω6c/C18:1 ω7c) as the major fatty acids. The major polar lipids were sphingoglycolipid, phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylcholine. Differential phenotypic characteristics, together with chemotaxonomic, phylogenetic and genomic distinctiveness, indicated that strain EB310T is distinguishable from other members of the genus Erythrobacter. On the basis of the data exhibited, strain EB310T is considered to represent a novel species of the genus Erythrobacter, for which the name Erythrobacter mangrovi sp. nov., is proposed. The type strain is EB310T (= KCTC 72109T = MCCC 1K03690T). The genomic DNA G + C content is 62.9 mol%.

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Abbreviations

ANI:

Average nucleotide identity

isDDH:

In silico DNA–DNA hybridization

BGCs:

Biosynthetic gene clusters

MA:

Marine agar 2216

MB:

Marine broth 2216

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Acknowledgements

This work was supported by the Science and Technology Basic Resources Investigation Program of China (2017FY100300), the Sea Mountain Environmental Management Plan and APEI Construction Project Research (DY135-E2-2-05), the National Natural Science Foundation of China (No. 31900003) and Zhejiang Provincial Natural Science Foundation of China (No. LQ19C010005).

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Authors and Affiliations

  1. Ocean College, Zhejiang University, Zhoushan, 316021, People’s Republic of China

    Yang-Hui Ye, Cen Yan & Min Wu

  2. College of Life Sciences, Zhejiang University, Hangzhou, 310058, People’s Republic of China

    Nusratgul Anwar, Maripat Xamxidin, Ran Zhang, Yan-Fang Nie & Zhe Zhao

  3. College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, People’s Republic of China

    Cong Sun

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Contributions

C.S and M.W. conceived and guided the research. Y.H.Y., M.X. and N.A. performed the research. R.Z., C.Y. and Z.Z. analyzed data. Y.H.Y. and Y.F.N. wrote the paper. All authors read and approved the manuscript.

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Correspondence to Cong Sun or Min Wu.

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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain EB310T are MT522623. Whole Genome Shotgun project of strain EB310T has been deposited at DDBJ/ENA/GenBank under the accession CP053921.

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Ye, YH., Anwar, N., Xamxidin, M. et al. Description of Erythrobacter mangrovi sp. nov., an aerobic bacterium from rhizosphere soil of mangrove plant (Kandelia candel). Antonie van Leeuwenhoek 113, 1425–1435 (2020). https://doi.org/10.1007/s10482-020-01451-0

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