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Complete Genome Sequence of Ralstonia Phage Remenis, a Member of Putative New Genus within the Siphoviridae

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Abstract

One of the most destructive diseases affecting potato production globally is bacterial wilt, caused by Ralstonia solanacearum. Options for controlling this pathogen are currently limited. Therefore, in this study, whole genome sequence was used to assess the potential of phage Reminis as biocontrol agent. No sequences related to undesirable genes including virulence factors, antibiotic resistance, or lysogenic mediated or toxin-coding genes were found in phage genome, this suggests that phage Reminis may be a potential candidate for future R. solanacearum control strategies.

Resumen

Una de las enfermedades mas destructivas que afectan la producción de papa globalmente es la marchitez bacteriana, causada por Ralstonia solanacearum. Las opciones para controlar a este patógeno son actualmente limitadas. Por ello, en este estudio, se usó la secuencia genómica para analizar el potencial del fago Reminis como agente de biocontrol. En el genoma del fago no se encontraron secuencias relacionadas con genes “indeseables” que incluyeran factores de virulencia, resistencia a antibiótico, genes de lisogenia o genes que codifiquen toxinas. Esto sugiere que el fago Reminis pudiera ser un candidato potencial para futuras estrategias de control de R. solanacearum.

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Acknowledgements

Support for the research was provided by the CONACYT (Project no. 251516). The autors appreciates the excellent technical assistance of Ing. Oscar Patrón and Luis Figueroa.

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Author notes

  1. Luis Amarillas and Mitzi Estrada-Acosta contributed equally to this work.

Authors and Affiliations

  1. Instituto de Investigación Lightbourn, A. C, Carretera Las Pampas km. 2.5, Ciudad Jiménez, Chihuahua, 33980, México

    Luis Amarillas, Rubén G. León-Chan & Luis Lightbourn

  2. Universidad Autónoma de Sinaloa, Culiacán, Sinaloa, México

    Mitzi Estrada-Acosta & Carlos López-Orona

  3. Centro de Investigación en Alimentación y Desarrollo, A. C., Culiacán, Sinaloa, México

    Josefina León-Félix

Authors
  1. Luis Amarillas
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  2. Mitzi Estrada-Acosta
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  3. Rubén G. León-Chan
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  4. Carlos López-Orona
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  5. Josefina León-Félix
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  6. Luis Lightbourn
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Corresponding author

Correspondence to Luis Lightbourn.

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Amarillas, L., Estrada-Acosta, M., León-Chan, R.G. et al. Complete Genome Sequence of Ralstonia Phage Remenis, a Member of Putative New Genus within the Siphoviridae. Am. J. Potato Res. 97, 447–449 (2020). https://doi.org/10.1007/s12230-020-09793-1

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  • DOI: https://doi.org/10.1007/s12230-020-09793-1

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