Abstract
Saccharomyces cerevisiae FT858 is an industrial yeast strain with high fermentative efficiency, but marginally studied so far. The aim of this work was to evaluate the biotechnological potential of S. cerevisiae FT858 through kinetic growth parameters, and the influence of the concentration of the substrate on the synthesis of the invertase enzyme. Invertases have a high biotechnological potential and their production through yeast is strongly influenced by the sugars in the medium. S. cerevisiae FT858 has an excellent biotechnological potential compared to the industrial yeast reference S. cerevisiae CAT-1, as it presented a low glycerol yield on the substrate (YGLY/S) and a 10% increase in ethanol yield on sucrose in cultures with sucrose at 37 °C. The substrate concentration directly interfered in invertase production and the enzymatic expression underwent strong regulation through glucose concentration in the culture medium and S. cerevisiae CAT-1 presented constitutive behavior for the invertase enzyme.
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Acknowledgments
The authors gratefully acknowledge the Pro-rectory of Research and Post-graduation of the Federal University of Grande Dourados and the Brazilian research funding agencies CAPES, CNPq and FUNDECT for their financial support.
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VMN: conceptualization, methodology, investigation, writing—original draft. GTUA: investigation. RSRL: methodology, visualization, formal analysis. GGF: project administration, supervision, visualization, writing—original draft, review & editing.
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Nascimento, V.M., Antoniolli, G.T.U., Leite, R.S.R. et al. Effects of the carbon source on the physiology and invertase activity of the yeast Saccharomyces cerevisiae FT858. 3 Biotech 10, 348 (2020). https://doi.org/10.1007/s13205-020-02335-w
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DOI: https://doi.org/10.1007/s13205-020-02335-w