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Cholecystokinin 1 and 2 in red seabream Pagrus major: molecular cloning, response to feeding, and a potential indicator of dietary protein source quality

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Abstract

Cholecystokinin (Cck) and peptide YY (Pyy) play important roles in digestive regulation in fish, but their function in red seabream (Pagrus major) remains obscure. Two cck complementary DNAs (cck-1 and cck-2) in red seabream were identified, and tissue distribution analysis of the genes revealed that cck and pyy were expressed throughout the digestive tract, except for stomach cck-2. After feeding, cck-1 expression significantly increased in the stomach and pyloric caeca, cck-2 expression significantly increased in the pyloric caeca and hindgut, and pyy expression increased in the pyloric caeca of red seabream. After oral administration of dietary protein, i.e., high-quality fish meal (HQFM), soy protein concentrate (SPC), or low-quality FM (LQFM), only HQFM significantly increased stomach cck-1 expression. To assess the growth of red seabream in response to different sources of dietary protein, three isonitrogenous and isoenergetic diets were formulated: HQFM, SPC with a low percentage of HQFM, and LQFM. Mean body weight was highest in the HQFM group after 6 weeks. The stomach cck-1 response to HQFM may explain the high growth in the HQFM group. Our data suggest that cck-1, cck-2, and pyy are involved in digestion. Furthermore, stomach cck-1 may be a valuable tool for evaluating the quality of dietary protein sources for red seabream.

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Acknowledgment

We thank Feed One for donating the protein sources and experimental diets. The authors declare no conflict of interest.

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Authors and Affiliations

  1. Faculty of Agriculture and Marine Science, Kochi University, Monobe 200, Kochi, 783-8502, Japan

    Tran Thi Mai Huong, Ayaka Senzui, Toshiro Matsumoto & Haruhisa Fukada

  2. Research Institute for Aquaculture No.1, Tu Son, Bac Ninh, Vietnam

    Tran Thi Mai Huong

  3. National Research Institute of Aquaculture, Japan Fisheries Research and Education Agency, Tamaki, Mie, 519-0423, Japan

    Koji Murashita

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  1. Tran Thi Mai Huong
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Correspondence to Haruhisa Fukada.

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Huong, T.T.M., Murashita, K., Senzui, A. et al. Cholecystokinin 1 and 2 in red seabream Pagrus major: molecular cloning, response to feeding, and a potential indicator of dietary protein source quality. Fish Sci 86, 835–849 (2020). https://doi.org/10.1007/s12562-020-01443-z

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