Abstract
Alfalfa (Medicago sativa L.) growing areas of Argentina were surveyed between 2010 and 2018 to determine the geographical distribution and analyse the genetic diversity among alfalfa enamovirus-1 (AEV-1) isolates. The virus was detected in all 17 surveyed alfalfa-producing provinces, with a prevalence of 64%. The plant virus AEV-1 is widely distributed in the country, and its transmission vector has been unknown until now. Here we show that the black aphid Aphis craccivora can transmit AEV-1. The CP sequence identity among 16 AEV-1 isolates from Argentina was from 98 to 100% and from 98.9 to 100% at nucleotide and amino acid levels, respectively, indicating a low level of sequence variation among these isolates. The phylogenetic analysis based on the complete nucleotide sequence of the CP gene indicated that AEV-1 isolates are closely related and clustered in a monophyletic group. These results suggest that AEV-1 has spread very recently in Argentina. In the present study, we report the geographical distribution of AEV-1 in the main alfalfa-growing areas of Argentina and, for the first time, identify an insect vector and describe the CP gene diversity of an enamovirus worldwide.
References
Bolsa de Cereales (2018). https://www.bolsadecereales.com/. Accessed 15 August 2019
Bolsa de Comercio de Rosario (2018) https://www.bcr.com.ar/Pages/gea/estimaProd.aspx. Accessed 15 August 2019
INTA-Informa. Descubren una nueva enfermedad en alfalfa (2010) https://intainforma.inta.gov.ar/?p=2693. Accessed 29 October 2015
Bejerman N, Nome C, Giolitti F, Kitajima E, de Breuil S, Pérez Fernández J, Basigalup D, Cornacchione M, Lenardon S (2011) First report of a Rhabdovirus infecting alfalfa in Argentina. Plant Dis 95(6):771. https://doi.org/10.1094/PDIS-10-10-0764
Trucco V, de Breuil S, Bejerman N, Lenardon S, Giolitti F (2014) Complete nucleotide sequence of Alfalfa mosaic virus isolated from alfalfa (Medicago sativa L.) in Argentina. Virus Genes 48(3):562–565. https://doi.org/10.1007/s11262-014-1045-0
Bejerman N, Giolitti F, Trucco V, de Breuil S, Dietzgen RG, Lenardon S (2016) Complete genome sequence of a new enamovirus from Argentina infecting alfalfa plants showing dwarfism symptoms. Arch Virol 161(7):2029–2032. https://doi.org/10.1007/s00705-016-2854-3
Bejerman N, Giolitti F, Trucco V, De Breuil S, Rodriguez Pardina P, Lenardon S. (2017) Caracterización genómica del Alfalfa leaf curl virus, un capulavirus que infecta el cultivo de alfalfa en Argentina. 19–21/04/17, Mendoza, Argentina: 4° Congreso Argentino de Fitopatología
Bejerman N, Giolitti F, de Breuil S, Trucco V, Nome C, Lenardon S, Dietzgen R (2015) Complete genome sequence and integrated protein localization and interaction map for alfalfa dwarf virus, which combines properties of both cytoplasmic and nuclear plant rhabdoviruses. Virology 483:275–283. https://doi.org/10.1016/j.virol.2015.05.001
Bejerman N, Debat H, Nome C, Cabrera Mederos D, Trucco V, de Breuil S, Lenardon S, Giolitti F (2019) Redefining the medicago sativa alphapartitiviruses genome sequences. Virus Res 265:156–161. https://doi.org/10.1016/j.virusres.2019.03.021
Trucco V, de Breuil S, Bejerman N, Lenardon S, Giolitti F (2016) Bean leafroll virus (BLRV) in Argentina: molecular characterization and detection in alfalfa fields. Eur J Plant Pathol 146:207–212. https://doi.org/10.1007/s10658-016-0899-5
Trucco VM, Bejerman N, de Breuil S, Cabrera Mederos D, Lenardon S, Giolitti F (2018) Alfalfa dwarf disease, a viral complex affecting alfalfa crop in Argentina. In: PROCEEDINGS OF THE SECOND WORLD ALFALFA CONGRESS. p. 84–7. https://www.worldalfalfacongress.org/resumenes/?lang=en
Ashby JW (1984) Bean leafroll virus N°286. Description of plant viruses. New Zealand, Plant Diseases Division, DSIR, Private Bag. https://www.dpvweb.net/dpv/showdpv.php?dpvno=286
Roumagnac P, Granier M, Bernardo P, Deshoux M, Ferdinand R, Galzi S, Fernandez E, Julian C, Abt I, Filloux D, Mesléard F, Varsani A, Blanc S, Martin D, Peterschmitt M (2015) Alfalfa Leaf Curl Virus: an Aphid-Transmitted Geminivirus. J Virol 89(18):9683–9688. https://doi.org/10.1128/JVI.00453-15
Jaspars EM, Bos L (1980) Alfalfa mosaic virus. N°229. In: Description of plant viruses. Kew, England, England: Commonwealth Mycology Institute/Association of Applied Biologists. https://www.dpvweb.net/dpv/showdpv.php?dpvno=229
Nibert ML, Ghabrial SA, Maiss E, Lesker T, Vainio EJ, Jiang D, Suzuki N (2014) Taxonomic reorganization of family Partitiviridae and other recent progress in partitivirus research. Virus Res 188:128–141. https://doi.org/10.1016/j.virusres.2014.04.007
Nemchinov LG, Grinstead SC, Mollov DS (2017) First report and complete genome sequence of alfalfa enamovirus from Sudan. Genome Announcements 5(27):e00531–e617. https://doi.org/10.1128/genomeA.00531-17
Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15
Nutter FW, Teng FM, Shokes FM (1991) Disease assessment terms and concepts. Plant Dis 75:1187–1188
Picard C, Dallot S, Brunker K, Berthier K, Roumagnac P, Soubeyrand S, Jacquot E, Thébaud G (2017) Exploiting genetic information to trace plant virus dispersal in landscapes. Annu Rev Phytopathol 55(1):139–160
Muhire BM, Varsani A, Martin DP (2014) SDT : a virus classification tool based on pairwise sequence alignment and identity calculation. PLoS ONE 9(9):e108277
Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: Molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35(6):1547–1549
Vandamme AM (2009) Basic concepts of molecular evolution. In: Lemey P, Salemi M, Vandamme AM, editors. The Phylogenetic Handbook. 2nd ed. Cambridge University Press. p. 3–29
Domier LL (2012) Family Luteoviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ, editors. Virus Taxonomy 9th Report of the International Committee on the Taxonomy of Viruses. Elsevier Academic Press, San Diego. p. 1045–53. https://www.ictvonline.org/
Bosco D, Tedeschi R (2013) Insect vector transmission assays. In: Phytoplasma: Methods and Protocols, Methods in Molecular Biology. p. 71–85
Bejerman N, Trucco V, de Breuil S, Pardina PR, Lenardon S, Giolitti F (2017) Genome characterization of an Argentinean isolate of alfalfa leaf curl virus. Arch Virol 5:1–5. https://doi.org/10.1007/s00705-017-3673-x
Gray S, Gildow FE (2003) Luteovirus-aphid interactions. Annu Rev Phytopathol 41(63):539–66. Available from: https://www.ncbi.nlm.nih.gov/pubmed/12730400
Mascia T, Gallitelli D (2016) Synergies and antagonisms in virus interactions. Plant Sci 252:176–192
Imwinkelried JM, Fava FD, Trumper EV (2013) Pulgones ( Hemiptera : Aphidoidea ) de la alfalfa. INTA EDS. p. 6
Ortego J, Durante MPM (2003) Acyrthosiphon (A.) loti (Hemiptera: Aphididae) hallado sobre alfalfa en la Argentina. Rev la Soc Entomológica Argentina 62(1–2): 32–34. https://www.scielo.org.ar/scielo.php?script=sci_abstract&pid=S0373-56802003000100004&lng=en&nrm=iso&tlng=en
Basigalup DH (2007) El cultivo de alfalfa en Argentina. In Eds. INTA. p. 176–85
Acknowledgements
We thank Valeria Arolfo (EEA Manfredi-INTA, Argentina) for providing the alfalfa seeds, Mónica Cornacchione (EEA Santiago del Estero-INTA, Argentina) for sending alfalfa samples and Florencia Moreno (IPAVE-CIAP-INTA, Argentina) for technical assistance.
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This work was partially supported by INTA.
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VT designed and performed experiments, analysed the results and wrote the initial draft of the manuscript. DCM, SL and FG have contributed to the design of the study, data collection, analysis of results, reviewing and editing the manuscript.
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Trucco, V., Cabrera Mederos, D., Lenardon, S. et al. Geographical distribution, genetic studies and vector transmission of alfalfa enamovirus-1 infecting alfalfa crop in Argentina. Virus Genes 56, 662–667 (2020). https://doi.org/10.1007/s11262-020-01783-z
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DOI: https://doi.org/10.1007/s11262-020-01783-z