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Characterization of dimethoate resistance in Oxycarenus hyalinipennis (Costa): resistance selection, cross-resistance to three insecticides and mode of inheritance

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Abstract

Dusky cotton bug Oxycarenus hyalinipennis (Costa) (Hemiptera: Lygaeidae) has been found to infesting cotton and other plants in Pakistan and some other countries. However, in Pakistan the increasing infestation of O. hyalinipennis was controlled with different insecticides, but some degree of resistance has been reported. Inheritance and cross-resistance of dimethoate resistance selection have not been documented in O. hyalinipennis previously. Therefore, this study for the first time characterized dimethoate resistance in O. hyalinipennis by inducing it in the laboratory for six generations. The selected population was 9.70- and 157-fold resistant compared with the field and laboratory population, respectively. The dimethoate resistance selection did not bring about any cross-resistance with lambda-cyhalothrin (1.47-fold) but had a very low cross-resistance with chlorfenapyr (3.03-fold) and acephate (2.21-fold) compared with the field population. The overlapping in 95% confidence limits of LC50 ratios of F1 (R♀ × S♂) and F1` (R♂ × S♀) indicated the lack of maternal effects, whereas their degree of dominance of 0.54 and 0.51 suggested an incompletely dominant resistance inheritance. Multiple factors were involved in dimethoate resistance as implied from Chi-square analyses of backcrosses. In conclusion, O. hyalinipennis develops easily resistance to dimethoate due to selection. Lack of/or very low cross-resistance with lambda-cyhalothrin, chlorfenapyr and acephate implies that these insecticides can be used in rotation. Moreover, dimethoate resistance inheritance was incompletely dominant, autosomal and polygenic which implies that resistance to dimethoate can be delayed by using it in rotations, alterations, mosaics and combinations.

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Acknowledgements

The authors are grateful to Prof. Dr. Jose Eduardo Serrao, Department of General Biology, Federal University of Viçosa, Brazil, for sparing his time to read the manuscript for English language and sense.

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Authors and Affiliations

  1. Department of Entomology, Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan, Pakistan

    Ansa Banazeer, Muhammad Babar Shahzad Afzal & Sarfraz Ali Shad

  2. Citrus Research Institute, Sargodha, Punjab, Pakistan

    Muhammad Babar Shahzad Afzal

Authors
  1. Ansa Banazeer
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  2. Muhammad Babar Shahzad Afzal
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  3. Sarfraz Ali Shad
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Contributions

AB conceived the study. MBSA and SAS helped to design the study. AB collected the population from field, reared the insects and performed laboratory work. SAS supervised the research work. AB and MBSA analyzed the data and wrote the manuscript. SAS read and approved the final version of the manuscript.

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Correspondence to Muhammad Babar Shahzad Afzal or Sarfraz Ali Shad.

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Banazeer, A., Afzal, M.B.S. & Shad, S.A. Characterization of dimethoate resistance in Oxycarenus hyalinipennis (Costa): resistance selection, cross-resistance to three insecticides and mode of inheritance. Phytoparasitica 48, 841–849 (2020). https://doi.org/10.1007/s12600-020-00831-6

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