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A symbiotic yeast to enhance heterotrophic and mixotrophic cultivation of Chlorella pyrenoidosa using sucrose as the carbon source

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Abstract

Heterotrophic or mixotrophic culture of microalgae is feasible alternative approach to avoid light limitation in autotrophic culture. However, only a few kinds of organic carbon sources are available for algal culture. Disaccharides, such as sucrose, are difficult to be utilized by microalgae under both heterotrophic and mixotrophic conditions. In this study, a symbiotic yeast was accidentally found in a contaminated algal suspension. The symbiotic yeast was isolated and identified as Cryptococcus sp. This yeast was able to extracellularly hydrolyze sucrose and accumulated monosaccharides in the medium. It can enhance algal growth using sucrose as the carbon source at both heterotrophic and mixotrophic modes when mix-cultured with Chlorella pyrenoidosa. The highest algal cell density of 118.8 × 106 and 151.2 × 106 cells/mL was achieved with a final algal percentage of 83.5 and 93.2% at heterotrophic and mixotrophic culture, respectively. Furthermore, the protein and lipid content was significantly enhanced by mix-culture C. pyrenoidosa with Cryptococcus YZU-1. The fatty acid accumulated in this co-culture system was suitable for the production of biodiesel. This symbiotic yeast solved the problem that C. pyrenoidosa cannot heterotrophically or mixotrophically utilize sucrose. A high algae density was obtained and the protein and lipid accumulation were also significantly enhanced. This study provided a novel approach for production of protein or lipid-rich biomass using sucrose or sucrose-rich wastes as the carbon source.

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Abbreviations

IAA:

Indole-3-acetic acid

MUFA:

Monounsaturated fatty acid

PUFA:

Polyunsaturated fatty acid

SFA:

Saturated fatty acid

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (no. 31802323), Natural Science Foundation of Jiangsu Province, China (no. BK20170495), and High-level Talent Support Program of Yangzhou University.

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  1. Yong-Ting Tian and Xu Wang contribute equally to this work.

Authors and Affiliations

  1. Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, 225009, Jiangsu, People’s Republic of China

    Yong-Ting Tian, Xu Wang, Yue-Hua Cui & Shi-Kai Wang

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  1. Yong-Ting Tian
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  2. Xu Wang
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Contributions

YT and XW carried out the experiments, analyzed the data and drafted the manuscript. YC contributed analytic methods. SW designed the experiments, analyzed the data and drafted the manuscript. All authors read and approved the final manuscript.

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Correspondence to Shi-Kai Wang.

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Tian, YT., Wang, X., Cui, YH. et al. A symbiotic yeast to enhance heterotrophic and mixotrophic cultivation of Chlorella pyrenoidosa using sucrose as the carbon source. Bioprocess Biosyst Eng 43, 2243–2252 (2020). https://doi.org/10.1007/s00449-020-02409-2

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