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Effect of selenium uptake on growth metabolism in yeasts for the production of enriched single-cell protein using agro-industrial by-products

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Abstract

The use of agro-industrial by-products as feedstock for selenium-enriched yeast biomass production is an innovative and low-cost method. Thus, the present study aims for the utilization of sugarcane bagasse and corn bran hydrolysates for the production of selenium-enriched yeast and the evaluation of the effect of selenium in growth and cell composition. The hydrolysates were obtained using acid pretreatment, and yeasts were evaluated in different medium compositions. All evaluated yeasts were able to grow in the presence of 15 mg/L selenium. S. cerevisiae strains presented the major tolerance to selenium and better relation between selenium uptake and cell growth, with maximum cell biomass production of 7.97 ± 0.24 g/L and maximum selenium uptake of 1193 ± 336 ppm and 99% cell viability. Selenium also enhanced the lipid and protein concentration in cell extracts obtained after mechanical disruption. Regarding agro-industrial hydrolysates, corn bran hydrolysate allowed a better growth, with the production of 4.25 ± 0.49 g/L of biomass enriched with 167 ± 18 ppm selenium and 100% of viability. Starchy and lignocellulosic biomasses presented potential as low-cost alternatives for single-cell protein contributing to the development of sustainable and economically viable technologies.

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Acknowledgments

The authors would like to thank Professor Carlos Augusto Rosa (UFMG, Brazil) and Professor Fernando Carlos Pagnocca (UNESP, Brazil) for the yeast strains.

Funding

This study received financial support provided by the Research Council for the State of São Paulo (Fundação de Amparo à Pesquisa do Estado de São Paulo—FAPESP—Process No. 2016/10636-8), the Coordination for the Improvement of Higher Education Personnel (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—USP-CAPES program), and the Council for Scientific and Technological Development (Conselho Nacional de Desenvolvimento Científico e Tecnológico—CNPq).

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Authors and Affiliations

  1. Engineering School of Lorena, University of São Paulo, Estrada Municipal do Campinho, s/n, Lorena, SP, 12602-810, Brazil

    Sabrina Evelin Martiniano, Rafael Rodrigues Philippini, Paulo Ricardo Franco-Marcelino & Silvio Silvério da Silva

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  1. Sabrina Evelin Martiniano
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  2. Rafael Rodrigues Philippini
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  3. Paulo Ricardo Franco-Marcelino
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  4. Silvio Silvério da Silva
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Correspondence to Sabrina Evelin Martiniano.

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Statement of novelty

Selenium is a trace element essential for both human and animal health, preventing and combating several diseases, and these benefits are increased when selenium is incorporated by yeasts. The use of agro-industrial by-products as feedstocks assists in the development of a more cost-benefit and eco-friendly biomolecules. The present study is the first report of selenium-enriched yeast production using agro-industrial by-products as both carbon and nitrogen sources. We also evaluated the effects of selenium uptake in different yeasts, generally used in animal feed. Sugarcane bagasse and corn bran hydrolysates as fermentative media enabled the production of yeast biomass enriched with selenium, presenting potential as low-cost alternatives for mineral-enriched single-cell protein and contributing to the development of sustainable and economically viable technologies.

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Martiniano, S.E., Philippini, R.R., Franco-Marcelino, P.R. et al. Effect of selenium uptake on growth metabolism in yeasts for the production of enriched single-cell protein using agro-industrial by-products. Biomass Conv. Bioref. 12, 3975–3983 (2022). https://doi.org/10.1007/s13399-020-00885-w

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