Abstract
Quantitative influence and underlying mechanisms of nutrient stoichiometry on mineralization of native Soil organic C (SOC) and straw C in different aggregate classes from cultivated and non-cultivated soils are still unclear. Soil samples (Mollisols) from a native woodlot and a farmland converted from woodlot were sieved into three aggregate classes (mega-aggregates (6.3-2 mm), macro-aggregates (2-0.25 mm), and micro-aggregates (< 0.25 mm)) and incubated (180 days) under different nutrient rates (nil, low, and high supplies of N and P) with or without 13C-enriched straw amendment. Significantly higher percentage of native SOC was mineralized from mega- and macro-aggregates (65.8-82.2%) compared with micro-aggregates (48.3-52.0%) in woodlot soil. Nutrient addition significantly increased aggregate-associated C in both soils with straw, and the increase was greater in farmland than in woodlot soil and in large-sized aggregates than in micro-aggregates. These results suggested that large aggregates serve as a C reservoir of labile C, while micro-aggregates that contained C was not easily mineralized even with abundant nutrients. Differences of C mineralization among aggregate size classes were significant in woodlot soil but not in farmland soil. Depletion of SOC was greater with increasing nutrient addition rates in farmland aggregates without straw, while the depletion in woodlot aggregates showed no difference among nutrient treatments, suggesting that microbial activity was nutrient-limited in farmland aggregates. The results improved our knowledge on SOC mineralization in response to residue-nutrient management in different aggregate classes from cultivated and non-cultivated soils, which have important implications for strategies to improve soil fertility or mitigate climate change via increased SOC.
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References
Balesdent J, Chenu C, Balabane M (2000) Relationship of soil organic matter dynamics to physical protection and tillage. Soil Tillage Res 53:215-230. https://doi.org/10.1016/s0167-1987(99)00107-5
Begum N, Guppy C, Herridge D, Schwenke G (2014) Influence of source and quality of plant residues on emissions of N2O and CO2 from a fertile, acidic black Vertisol. Biol Fertil Soils 50:499-506. https://doi.org/10.1007/s00374-013-0865-8
Bimüller C, Kreyling O, Kölbl A, von Lützow M, Kögel-Knabner I (2016) Carbon and nitrogen mineralization in hierarchically structured aggregates of different size. Soil Tillage Res 160:23-33. https://doi.org/10.1016/j.still.2015.12.011
Bonanomi G, Incerti G, Giannino F, Mingo A, Lanzotti V, Mazzoleni S (2013) Litter quality assessed by solid state 13C NMR spectroscopy predicts decay rate better than C/N and lignin/N ratios. Soil Biol Biochem 56:40-48. https://doi.org/10.1016/j.soilbio.2012.03.003
Butterly CR, Armstrong RD, Chen D, Tang CX (2018) Residue decomposition and soil carbon priming in three contrasting soils previously exposed to elevated CO2. Biol Fert Soils 55:17-29. https://doi.org/10.1007/s00374-018-1321-6
Chen R, Senbayram M, Blagodatsky S, Myachina O, Dittert K, Lin X, Blagodatskaya E, Kuzyakov Y (2014) Soil C and N availability determine the priming effect: microbial N mining and stoichiometric decomposition theories. Global Change Biol 20:2356-2367. https://doi.org/10.1111/gcb.12475
Chen H, Dong Y, Xu T, Wang Y, Wang H, Duan B (2017) Root order-dependent seasonal dynamics in the carbon and nitrogen chemistry of poplar fine roots. New For 48(5):587-607. https://doi.org/10.1007/s11056-017-9587-3
Christopher SF, Lal R (2007) Nitrogen management affects carbon sequestration in North American cropland soils. Crit Rev Plant Sci 26:45-64. https://doi.org/10.1080/07352680601174830
Chung H, Ngo KJ, Plante A, Six J (2010) Evidence for carbon saturation in a highly structured and organic-matter-rich soil. Soil Sci Soc Am J 74:130-138. https://doi.org/10.2136/sssaj2009.0097
Cotrufo MF, Soong JL, Horton AJ, Campbell EE, Haddix M, Wall DH, Parton WJ (2015) Formation of soil organic matter via biochemical and physical pathways of litter mass loss. Nat Geosci 8:776-779. https://doi.org/10.1038/ngeo2520
Craine JM, Morrow C, Fierer NO (2007) Microbial nitrogen limitation increases decomposition. Ecology 88:2105-2113. https://doi.org/10.1890/06-1847.1
Dai W, Fu W, Jiang P, Zhao K, Li Y, Tao J (2018) Spatial pattern of carbon stocks in forest ecosystems of a typical subtropical region of southeastern China. Forest Ecol Manag 409:288-297. https://doi.org/10.1016/j.foreco.2017.11.036
Dawson TE, Mambelli S, Plamboeck AH, Templer PH, Tu KP (2002) Stable isotopes in plant ecology. Annu Rev Ecol Syst 33:507-559. https://doi.org/10.1146/annurev.ecolsys.33.020602.095451
de Graaff MA, van Groenigen KJ, Six J, Hungate B, van Kessel C (2006) Interactions between plant growth and soil nutrient cycling under elevated CO2: a meta-analysis. Glob Chang Biol 12:2077-2091. https://doi.org/10.1111/j.1365-2486.2006.01240.x
de Sosa LL, Glanville HC, Marshall MR, Schnefp A, Cooper DM, Hill PW, Binley A, Jones DL (2018) Stoichiometric constraints on the microbial processing of carbon with soil depth along a riparian hillslope. Biol Fert Soils 54:949-963. https://doi.org/10.1007/s00374-018-1317-2
Demoling F, Figueroa D, Bååth E (2007) Comparison of factors limiting bacterial growth in different soils. Soil Biol Biochem 39:2485-2495. https://doi.org/10.1016/j.soilbio.2007.05.002
Denef K, Six J, Bossuyt H, Frey SD, Elliott ET, Merckx R, Paustian K (2001) Influence of wet-dry cycles on the interrelationship between aggregate, particulate organic matter, and microbial community dynamics. Soil Biol Biochem 33:1599-1611. https://doi.org/10.1016/S0038-0717(01)00076-1
Denef K, Zotarelli L, Boddey RM, Six J (2007) Microaggregate-associated carbon as a diagnostic fraction for management-induced changes in soil organic carbon in two Oxisols. Soil Biol Biochem 39:1165-1172. https://doi.org/10.1016/j.soilbio.2006.12.024
Ding X, Zhang B, Wei Z, He H, Filley TR (2020) Conversion of grassland into cropland affects microbial residue carbon retention in both surface and subsurface soils of a temperate agroecosystem. Biol Fert Soils 56:137-143. https://doi.org/10.1007/s00374-019-01400-8
Drury CF, Yang XM, Reynolds WD, Tan CS (2004) Influence of crop rotation and aggregate size on carbon dioxide production and denitrification. Soil Tillage Res 79:87-100. https://doi.org/10.1016/j.still.2004.03.020
Fan RQ, Yang XM, Drury CF, Guo XB, Zhang XP (2013) Distribution and stability of organic carbon in soil aggregate external and internal layers under three different land-use systems. Soil Sci Soc Am J 77:1625-1635. https://doi.org/10.2136/sssaj2013.03.0086
Fan YX, Zhong XJ, Lin TC, Lyu MK, Wang MH, Hu WF, Yang ZJ, Chen GS, Guo JF, Yang YS (2019) Effects of nitrogen addition on DOM-induced soil priming effects in a subtropical plantation forest and a natural forest. Biol Fert Soils 56:205-216. https://doi.org/10.1007/s00374-019-01416-0
Fan RQ, Zhang BH, Li JY, Zhang ZH, Liang AZ (2020) Straw-derived biochar mitigates CO2 emission through changes in soil pore structure in a wheat-rice rotation system. Chemosphere 243:125329. https://doi.org/10.1016/j.chemosphere.2019.125329
Fang YY, Singh BP, Badgery W, He X (2016) In situ assessment of new carbon and nitrogen assimilation and allocation in contrastingly managed dryland wheat crop-soil systems. Agric Ecosyst Environ 235:80-90. https://doi.org/10.1016/j.agee.2016.10.010
Fang YY, Singh BP, Collins D, Li B, Zhu J, Tavakkoli E (2018) Nutrient supply enhanced wheat residue-carbon mineralization, microbial growth, and microbial carbon-use efficiency when residues were supplied at high rate in contrasting soils. Soil Biol Biochem 126:168-178. https://doi.org/10.1016/j.soilbio.2018.09.003
Fang YY, Singh BP, Cowie A, Wang WQ, Arachchi MH, Wang HL, Tavakkoli E (2019) Balancing nutrient stoichiometry facilitates the fate of wheat residue-carbon in physically defined soil organic matter fractions. Geoderma 354:113883. https://doi.org/10.1016/j.geoderma.2019.113883
Gunina A, Kuzyakov Y (2014) Pathways of litter C by formation of aggregates and SOM density fractions: implications from 13 C natural abundance. Soil Biol Biochem 71:95-104. https://doi.org/10.1016/j.soilbio.2014.01.011
Hassink J, Whitmore AP (1997) A model of the physical protection of organic matter in soils. Soil Sci Soc Am J 61:131-139. https://doi.org/10.2136/sssaj1997.03615995006100010020x
He MZ, Dijkstra FA (2015) Phosphorus addition enhances loss of nitrogen in a phosphorus-poor soil. Soil Biol Biochem 82:99-106. https://doi.org/10.1016/j.soilbio.2014.12.015
Herron PM, Stark JM, Holt C, Hooker T, Cardon ZG (2009) Microbial growth efficiencies across a soil moisture gradient assessed using 13C-acetic acid vapour and 15N-ammonia gas. Soil Biol Biochem 41:1262-1269. https://doi.org/10.1016/j.soilbio.2009.03.010
Heuck C, Weig A, Spohn M (2015) Soil microbial biomass C:N:P stoichiometry and microbial use of organic phosphorus. Soil Biol Biochem 85:119-129. https://doi.org/10.1016/j.soilbio.2015.02.029
Himes FL (1998) Nitrogen, sulfur, and phosphorus and the sequestering of carbon. In: Lal R, Kimble JM, Follett RF, Stewart BA (eds) Soil processes and the carbon cycle. CRC Press, Boca Raton, FL, pp 315-319
Ilstedt U, Singh S (2005) Nitrogen and phosphorous limitations of microbial respiration in a tropical phosphorous-fixing acrisol (ultisol) compared with organic compost. Soil Biol Biochem 37:1407-1410. https://doi.org/10.1016/j.soilbio.2005.01.002
Janssens IA, Dieleman W, Luyssaert S, Subke JA, Reichstein M, Ceulemans R, Ciais P, Dolman AJ, Grace J, Matteucci G, Papale D, Piao SL, Schulze ED, Tang J, Law BE (2010) Reduction of forest soil respiration in response to nitrogen deposition. Nat Geosci 3:315-322. https://doi.org/10.1038/ngeo844
Kallenbach CM, Frey SD, Grandy AS (2016) Direct evidence for microbial-derived soil organic matter formation and its ecophysiological controls. Nat Commun 7:13630. https://doi.org/10.1038/ncomms13630
Kirkby CA, Kirkegaard JA, Richardson AE, Wade LJ, Blanchard C, Batten G (2011) Stable soil organic matter: a comparison of C:N:P:S ratios in Australian and other world soils. Geoderma 163:197-208. https://doi.org/10.1016/j.geoderma.2011.04.010
Kirkby CA, Richardson AE, Wade LJ, Batten GD, Blanchard C, Kirkegaard JA (2013) Carbon-nutrient stoichiometry to increase soil carbon sequestration. Soil Biol Biochem 60:77-86. https://doi.org/10.1016/j.soilbio.2013.01.011
Lal R (2004) Soil carbon sequestration impacts on global climate change and food security. Science 304:1623-1627. https://doi.org/10.1126/science.1097396
Li J, Zhao BQ, Li XY, Hwat BS (2008) Effects of long-term combined application of organic and mineral fertilizers on soil microbiological properties and soil fertility. Sci Agric Sin 41:144-152. https://doi.org/10.3724/SP.J.1005.2008.01083
Li LJ, Xia ZB, Ye RZ, Doane TA, Horwath WR (2018) Soil microbial biomass size and soil carbon influence the priming effect from carbon inputs depending on nitrogen availability. Soil Biol Biochem 119:41-49. https://doi.org/10.1016/j.soilbio.2018.01.003
Liang AZ, Zhang XP, Yang XM, McLaughlin NB, Shen Y, Li WF (2009) Estimation of total erosion in cultivated black soils in Northeast China from vertical profiles of soil organic carbon. Eur J Soil Sci 60:223-229. https://doi.org/10.1111/j.1365-2389.2008.01100.x
Liljeroth E, Kuikman P, Veen JA (1994) Carbon translocation to the rhizosphere of maize and wheat and influence on the turnover of native soil organic matter at different soil nitrogen levels. Plant Soil 161:233-240. https://doi.org/10.1007/bf00046394
Limon-Ortega A, Govaerts B, Deckers J, Sayre KD (2006) Soil aggregate and microbial biomass in a permanent bed wheat-maize planting system after 12 years. Field Crop Res 97:302-309. https://doi.org/10.1016/j.fcr.2005.11.001
Liu SY, Fan RQ, Yang XM, Zhang ZH, Zhang XP, Liang AZ (2019) Decomposition of maize stover varies with maize type and stover management strategies: a microcosm study on a black soil (Mollisol) in Northeast China. J Environ Manag 234:226-236. https://doi.org/10.1016/j.jenvman.2019.01.008
Luo Y, Zang HD, Yu ZY, Chen ZY, Gunina A, Kuzyakov Y, Xu JM, Zhang KL, Brookes PC (2017) Priming effects in biochar enriched soils using a three-source-partitioning approach: 14C labelling and 13C natural abundance. Soil Biol Biochem 106:28-35. https://doi.org/10.1016/j.soilbio.2016.12.006
Mack MC, Schurr EAG, Bret-Harte MS, Shaver GR, Chapin FS III (2004) Ecosystem carbon storage in arctic tundra reduced by long-term nutrient fertilization. Nature 431:440-443. https://doi.org/10.1038/nature02887
Manlay RJ, Feller C, Swift MJ (2007) Historical evolution of soil organic matter concepts and their relationships with the fertility and sustainability of cropping systems. Agric Ecosyst Environ 119:217-233. https://doi.org/10.1016/j.agee.2006.07.011
Nordin A, Schmidt IK, Shaver GR (2004) Nitrogen uptake by arctic soil microbes and plants in relation to soil nitrogen supply. Ecology 85:955-962. https://doi.org/10.1890/03-0084
Piaszczyk W, Błońska E, Lasota J, Lukac M (2019) A comparison of C:N:P stoichiometry in soil and deadwood at an advanced des stage. Catena 179:1-5. https://doi.org/10.1016/j.catena.2019.03.025
Poeplau C, Don A, Vesterdal L, Leifeld L (2011) Temporal dynamics of soil organic carbon after land-use change in the temperate zone-carbon response functions as a model approach. Glob Chang Biol 17:2415-2427. https://doi.org/10.1111/j.1365-2486.2011.02408.x
Qiao N, Wang J, Xu X, Shen Y, Long X, Hu Y, Schaefer D, Li SG, Wang HM, Kuzyakov Y (2019) Priming alters soil carbon dynamics during forest succession. Biol Fert Soils 55:339-350. https://doi.org/10.1007/s00374-019-01351-0
Ros GH, Hoffland E, Temminghoff EJM (2010) Dynamics of dissolved and extractable organic nitrogen upon soil amendment with crop residues. Soil Biol Biochem 42:2094-2101. https://doi.org/10.1016/j.soilbio.2010.08.004
Sarker JR, Singh BP, Cowie AL, Fang YY, Collins D, Dougherty WJ, Singh BK (2018) Carbon and nutrient mineralisation dynamics in aggregate-size classes from different tillage systems after input of canola and wheat residues. Soil Biol Biochem 116:22-38. https://doi.org/10.1016/j.soilbio.2017.09.030
Shi XH, Zhang XP, Yang XM, Drury CF, McLaughlin NB, Liang AZ, Fan RQ, Jia SX (2012) Contribution of winter soil respiration to annual soil CO2 emission in a Mollisol under different tillage practices in Northeast China. Global Biogeochem Cy 26:GB2007. https://doi.org/10.1029/2011GB004054
Six J, Elliott ET, Paustian K (2000) Soil macroaggregate turnover and microaggregate formation: a mechanism for C sequestration under no-tillage agriculture. Soil Biol Biochem 32:2099-2103. https://doi.org/10.1016/s0038-0717(00)00179-6
Six J, Conant RT, Paul EA, Paustian K (2002) Stabilization mechanisms of soil organic matter: implications for C-saturation of soils. Plant Soil 241:155-176. https://doi.org/10.1023/a:1016125726789
Sun J, Gao P, Xu H, Li C, Niu X (2019) Decomposition dynamics and ecological stoichiometry of Quercus acutissima and Pinus densiflora litter in the grain to green program area of northern China. J Forestry Res:1-11. https://doi.org/10.1007/s11676-019-00981-2
Tian J, Pausch J, Yu G, Blagodatskaya E (2016) Aggregate size and glucose level affect priming sources: a three-source-partitioning study. Soil Biol Biochem 97:199-210. https://doi.org/10.1016/j.soilbio.2016.03.013
Tisdall JM, Oades JM (1982) Organic matter and water-stable aggregates in soils. J Soil Sci 33:141-163. https://doi.org/10.1111/j.1365-2389.1982.tb01755.x
Vance ED, Brookes PC, Jenkinson DS (1987) An extraction method for measuring soil microbial biomass C. Soil Biol Biochem 19:703-707. https://doi.org/10.1016/0038-0717(87)90052-6
Wang W, Sardans J, Zeng C, Zhong C, Li Y, Peñuelas J (2014) Responses of soil nutrient concentrations and stoichiometry to different human land uses in a subtropical tidal wetland. Geoderma 232-234:459-470. https://doi.org/10.1016/j.geoderma.2014.06.004
Wang GQ, Hao SS, Gao B, Chen MX, Liu YG, Yang JC, Ye NH, Zhang JH (2017) Regulation of gene expression in the remobilization of carbon reserves in rice stems during grain filling. Plant Cell Physiol 58(8):1391-1404
Wei X, Shao M, Gale W, Li L (2014) Global pattern of soil carbon losses due to the conversion of forests to agricultural land. Sci Rep 4:4062. https://doi.org/10.1038/srep04062
Wu H, Wiesmeier M, Yu Q, Steffens M, Han X, Kögel-Knabner I (2012) Labile organic C and N mineralization of soil aggregate size classes in semiarid grasslands as affected by grazing management. Biol Fert Soils 48:305-313. https://doi.org/10.1007/s00374-011-0627-4
Yu Z, Chen L, Pan S, Li Y, Kuzyakov Y, Xu J, Brookes PC, Luo Y (2018) Feedstock determines biochar-induced soil priming effects by stimulating the activity of specific microorganisms. Eur J Soil Sci 69:521-534. https://doi.org/10.1111/ejss.12542
Yuan HZ, Liu SL, Razavi BS, Zhran M, Wang JR, Zhu ZK, Wu JS, Ge TD (2019) Differentiated response of plant and microbial C:N:P stoichiometries to phosphorus application in phosphorus-limited paddy soil. Eur J Soil Sci 95:103-122. https://doi.org/10.1016/j.ejsobi.2019.103122
Zhang A, Liu Y, Pan G, Hussain Q, Li L, Zheng J, Zhang X (2012) Effect of biochar amendment on maize yield and greenhouse gas emissions from a soil organic carbon poor calcareous loamy soil from Central China Plain. Plant Soil 1-2:263-275. https://doi.org/10.1007/s11104-011-0957-x
Zhang Y, Li XL, Gregorich EG, McLaughlin NB, Zhang XP, Guo YF, Liang AZ, Fan RQ, Sun BJ (2018) No-tillage with continuous maize cropping enhances soil aggregation and organic carbon storage in Northeast China. Geoderma 330:204-211. https://doi.org/10.1016/j.geoderma.2018.05.037
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Financial supports for this research from the National Natural Science Foundation of China (41401259), the Natural Science Foundation of Jiangsu Province (BK20161379), and the China Postdoctoral Science Foundation (2018M632253) are gratefully acknowledged.
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Fan, R., Du, J., Liang, A. et al. Carbon sequestration in aggregates from native and cultivated soils as affected by soil stoichiometry. Biol Fertil Soils 56, 1109–1120 (2020). https://doi.org/10.1007/s00374-020-01489-2
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DOI: https://doi.org/10.1007/s00374-020-01489-2