Abstract
Heat stress increases the core body temperature through the pathogenic process. The pathogenic process leads to the release of free radicals, such as superoxide production. Heat stress in the central nervous system (CNS) can cause neuronal damage and symptoms such as delirium, coma, and convulsion. TRPV1 (Transient Receptor Potential Vanilloid1) and TRPV4 genes are members of the TRPV family, including integral membrane proteins that act as calcium-permeable channels. These channels act as thermosensors and have essential roles in the cellular regulation of heat responses. The objective of this study is to examine the effect of general heat stress on the expression of TRPV1 and TRPV4 channels. Furthermore, oxidative markers were measured in the brain of the same heat-stressed mice. Our results show that heat stress leads to a significant upregulation of TRPV1 expression within 21–42 days, while TRPV4 expression decreased significantly in a time-dependent manner. Alterations in the oxidative markers were also observed in the heat-stressed mice.
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Acknowledgements
We thank the Neuroscience Research Center of the Tabriz University of Medical Science. Dr. Mehdi Farhoudi’s Lab, from the East Azerbaijan Science and Technology Park and Dr. Pouran Karimi Lab in Tabriz Iran for their cooperation and support. And we also thank Dr. Anna Garcia-Elias from the Montreal Heart Institute in Canada for her advice.
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MAHF, LMF conceived and designed the study. AA, MG carried out the experiments, acquired the results, MG designed used primers. AA, LMF, MG analyzed and explicated results and drafted the manuscript. LR prepared tissue sections and analyzed the immunohistochemistry results. Each named author reviewed and approved the manuscript. All authors confirm that this manuscript has not been previously published and is not currently under consideration by any other journal.
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Aghazadeh, A., Feizi, M.A.H., Fanid, L.M. et al. Effects of Hyperthermia on TRPV1 and TRPV4 Channels Expression and Oxidative Markers in Mouse Brain. Cell Mol Neurobiol 41, 1453–1465 (2021). https://doi.org/10.1007/s10571-020-00909-z
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DOI: https://doi.org/10.1007/s10571-020-00909-z