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Overexpression of Long Non-Coding RNA FGF14-AS2 Inhibits Colorectal Cancer Proliferation Via the RERG/Ras/ERK Signaling by Sponging microRNA-1288-3p

  • Original Article
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Pathology & Oncology Research

Abstract

Colorectal cancer remains one of most common cancer types with poor prognosis globally. Recent years, numerous studies depicted pivotal roles of lncRNAs in colorectal cancer progression. This study aimed to investigate the role of FGF14-AS2 in colorectal cancer development. FGF14-AS2 was found as a significantly downregulated lncRNA in TCGA dataset. Via RT-qPCR, we confirmed the downregulation of FGF14-AS2 in collected colorectal carcinoma samples. Transfection of plasmid containing full length of FGF14-AS2 repressed cell proliferation and induced elevation of cell apoptosis in colorectal cancer cells. In addition, FGF14-AS2 overexpression inactivated MAPK/ERK signaling in cells. Bioinformatic analysis and subsequent cell-based assays showed that FGF14-AS2 sponging miR-1288-3p, an oncogenic miRNA in colorectal cancer. RERG, the regulator of Ras/ERK pathway, was predicted and verified as target gene of miR-1288. Via downregulation of miR-1288, FGF14-AS2 elevated RERG expression in colorectal cancer cells. Rescue assays indicated that FGF14-AS2 relied on regulation of RERG to control cell proliferation and apoptosis in colorectal cancer. Taken together, the current study demonstrated FGF14-AS2 as a regulator of colorectal cancer development via downregulation of miR-1288-3p and inactivation of Ras/ERK signaling.

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All data generated or analyzed during this study are included in this published article.

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Authors and Affiliations

  1. Department of Gastrointestinal Colorectal Surgery, the Third Hospital of Jilin University, Jilin University, Changchun, Jilin, 130033, China

    Ruizhi Hou, Yanzhuo Su & Zhenbo Shu

  2. Department of Ultrasound, the Third Hospital of Jilin University, Jilin University, Changchun, Jilin, 130033, China

    Yan Liu

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  1. Ruizhi Hou
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  2. Yan Liu
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Contributions

Ruizhi Hou, Yan Liu, Yanzhuo Su, Zhenbo Shu acquired data and performed data analysis. Ruizhi Hou and Yan Liu designed the study. Yanzhuo Su and Zhenbo Shu wrote the manuscript. Study was supervised by Zhenbo Shu.

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Correspondence to Zhenbo Shu.

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The study was performed in accordance with the Declaration of Helsinki and obtained the approval from ethical approval board of the Third Hospital of Jilin University.

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Hou, R., Liu, Y., Su, Y. et al. Overexpression of Long Non-Coding RNA FGF14-AS2 Inhibits Colorectal Cancer Proliferation Via the RERG/Ras/ERK Signaling by Sponging microRNA-1288-3p. Pathol. Oncol. Res. 26, 2659–2667 (2020). https://doi.org/10.1007/s12253-020-00862-8

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  • DOI: https://doi.org/10.1007/s12253-020-00862-8

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