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Physiological and morphological responses of blueberry to manganese stress in soil

  • Biochemistry & Physiology - Original Article
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Abstract

The effects of manganese (Mn) stress on photosynthetic pigment and malondialdehyde content, Mn accumulation, ascorbate (AsA), reduced glutathione (GSH), soluble sugar, sucrose, phytohormones metabolism, as well as some morphological characteristics of leaves were investigated in blueberry cultivar ‘Tifblue’ (Vaccinium ashei Reade) after 60 days treated by 1.0 mM, 2.5 mM, 5.0 mM, and 10.0 mM MnSO4 in soil. Mn accumulation increased in a concentration-dependent manner. A significant increase in MDA content was observed, except in the 1.0 mM and 2.5 mM Mn treatments. Levels of chlorophyll a (Chl a) and chlorophyll b (Chl b) first increased and then decreased, while carotenoid (Car) content decreased gradually. Mn stress induced a remarkable decrease in AsA content, but no significant change was observed in GSH. Soluble sugar content changed slightly, whereas sucrose content rose gradually with the increase in Mn concentration, reaching peak value at 5.0 mM. Jasmonic acid (JA) and salicylic acid (SA) contents increased alternately in response to Mn treatments, while 1-aminocylopropane-1-carboxylic acid content changed in a similar way to soluble sugar. Significant damage to palisade tissue cells and sponge tissue cells and stomatal closure imposed by high Mn concentration indicated a general disarray in the cellular functions. Our results suggested that Mn tolerance is regulated by mechanisms through multiple factors and is associated with elevated levels of photosynthetic pigments, soluble sugar, sucrose, JA, and SA. Besides, the critical value of Mn in soil on blueberry cultivar ‘Tifblue’ was 2.5 mM.

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Acknowledgements

This research was supported by National Natural Science Foundation of China (No.31700606) and the Foundation of Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (No. JSPKLB201608).

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SSD conducted the experiment, collected and analyzed the data, and prepared the draft. HYY, WLW, and WL Li participated in the design of the study and revised the manuscript. LFL helped in measurements of antioxidant enzymes. All authors read and approved the final manuscript.

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Correspondence to H. Y. Yang or W. L. Li.

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Dong, S.S., Yang, H.Y., Wu, W.L. et al. Physiological and morphological responses of blueberry to manganese stress in soil. Braz. J. Bot 43, 419–427 (2020). https://doi.org/10.1007/s40415-020-00625-4

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  • DOI: https://doi.org/10.1007/s40415-020-00625-4

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