Skip to main content
SpringerLink
Log in

Poly (ADP-ribose) polymerase inhibitor 3-methoxybenzamide enhances in vitro plant growth, microtuberization, and transformation efficiency of blue potato (Solanum tuberosum L. subsp. andigenum)

  • Micropropagation
  • Published:
In Vitro Cellular & Developmental Biology - Plant Aims and scope Submit manuscript

Abstract

Poly(ADP-ribose) polymerases (PARP) are involved in the regulation of plant growth and development, especially under stress conditions. These enzymes post-translationally modify target proteins by adding ADP-ribose polymers. This study presents the effect of PARP chemical inhibitor 3-methoxybenzamide (3 MB) on in vitro plant growth, microtuber formation, and Agrobacterium-mediated transformation of blue potato (Solanum tuberosum L. subsp. andigenum). The addition of 3 MB (0.2 to 0.6 mM) significantly increased the growth and microtuber formation of in vitro propagated plants. The highest gain in plant fresh weight, root mass, and microtuberization was observed in clonal propagation medium with 0.4 mM 3 MB. Transformation frequency of blue potato internodes increased to 43%, 2.7-fold over the non-treated control (16%), when 0.2 mM 3 MB was included in the culture medium during the whole process. The addition of 3 MB during Agrobacterium transformation did not affect transgene copy number in regenerated plants. Southern blot analyses and histochemical staining for ß-glucuronidase activity confirmed the presence and expression, respectively, of the uidA transgene in plant tissues. From these results, it can be concluded that the inhibition of PARP activity can increase biomass production and transformation rates for genetic improvement of blue potato.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1.
Figure 2.
Figure 3.
Figure 4.
Figure 5.
Figure 6.
Figure 7.

Similar content being viewed by others

References

  • Ahlfors R, Lang S, Overmyer K, Jaspers P, Brosche M, Tauriainen A, Kollist H, Tuominen H, Belles-Boix E, Piippo M, Inze D, Palva T, Kangasjarvi J (2004) Arabidopsis RADICAL-INDUCED CELL DEATH1 belongs to the WWE protein-protein interaction domain protein family and modulates abscisic acid, ethylene, and methyl jasmonate responses. Plant Cell 16:1925–1937

    Article  CAS  Google Scholar 

  • Altpeter F, Springer NM, Bartley L, Blechl AE, Brutnell TP, Citovsky V, Conrad LJ, Gervin SB, Jackson DP, Kausch AP, Lemaux PG, Medford JI, Orozco-Cárdenas ML, Tricoli DM, Van Eck J, Voytas DF, Walbot V, Wang K, Zhang ZJ, Stewart N (2016) Advancing crop transformation in the era of genome editing. Plant Cell 28:1510–1520

    CAS  PubMed  PubMed Central  Google Scholar 

  • Amor Y, Babiychuk E, Inzé D, Levine A (1998) The involvement of poly (ADP-ribose) polymerase in the oxidative stress responses in plants. FEBS Lett 440:1–7

    Article  CAS  Google Scholar 

  • Banerjee AK, Prat S, Hannapel DJ (2006) Efficient production of transgenic potato (S. tuberosum L. ssp. andigena) plants via Agrobacterium tumefaciens- mediated transformation. Plant Sci 170:732–738

    Article  CAS  Google Scholar 

  • Briggs AG, Bent A (2011) Poly(ADP-ribosyl)ation in plants. Trends Plant Sci 16:372–380

    Article  CAS  Google Scholar 

  • Chen P-Y, Wang C-K, Soong S-C, To K-Y (2003) Complete sequence of the binary vector pBI121 and its application in cloning T-DNA insertion from transgenic plants. Mol Breed 11:287–293

    Article  CAS  Google Scholar 

  • Chetty VJ, Ceballos N, García D, Narváez-Vásquez J, López W, Orozco-Cárdenas ML (2013) Evaluation of four Agrobacterium tumefaciens strains for the genetic transformation of tomato (Solanum lycopersicum L.) cultivar micro-tom. Plant Cell Rep 32:239–247

    Article  CAS  Google Scholar 

  • Chetty VJ, Narváez-Vásquez J, Orozco-Cárdenas ML (2015) Potato (Solanum tuberosum L.). In: Wang K (ed) Methods in Mol. Biol. Agrobacterium protocols Springer. Human Press, NY, pp 85–96

    Chapter  Google Scholar 

  • De Block M (2000) Genetic transformation using a PARP inhibitor. US patent WO 6074876

  • De Block M, Verduyn C, Brouwer D, Cornelissen M (2005) Poly (ADP-ribose) polymerase in plants affects energy homeostasis, cell death and stress tolerance. Plant J 41:95–106

    Article  Google Scholar 

  • Den BB (2010) High oil contents plants. US patent WO 2010142424A1

  • Ding R, Pommier Y, Kang VH, Smulson M (1992) Depletion of poly (ADP ribose) polymerases by antisense RNA expression results in a delay in DNA strand rejoining. J Biol Chem 267:12804–12812

    CAS  PubMed  Google Scholar 

  • Donnelli D, Coleman WK, Coleman SH (2003) Potato microtuber performance and production: a review. Am J Potato Res 80:103–115

    Article  Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158

    Article  CAS  Google Scholar 

  • Hansen G (2000) Evidence for Agrobacterium-induced apoptosis in maize. Mol Plant Microbe Interact 13:649–657

    Article  CAS  Google Scholar 

  • Hussain I, Chaudhry Z, Muhammad A, Asghar R, Naqvi SMS, Rashid H (2006) Effect of chlorocholine chloride, sucrose, and BAP on in vitro tuberization in potato (Solanum tuberosum L. cv. Cardinal). Pak J Bot 38:275–282

    Google Scholar 

  • Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusion: glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6:3901–3909

    Article  CAS  Google Scholar 

  • Khanna HK, Paul JY, Harding RM, Dickman MB, Dale JL (2007) Inhibition of Agrobacterium-induced cell death by antiapoptotic gene expression leads to very high transformation efficiency of banana. Mol Plant Microbe Interact 20:1048–1054

    Article  CAS  Google Scholar 

  • Lachman J, Hamaouz K (2005) Red and purple potatoes as a significant antioxidant source in human nutrition – a review. Plant Soil Environ 51:477–482

    Article  CAS  Google Scholar 

  • Lamb RS, Citarelli M, Teotia S (2012) Functions of the poly (ADP-ribose) polymerase superfamily in plants. Cell Mol Life Sci 69:175–189

    Article  CAS  Google Scholar 

  • Liu C, Wu Q, Liu W, Gu Z, Wang W, Xu P, Ma H, Ge X (2017) Poly (ADP-ribose) polymerase regulates cell division and development in Arabidopsis roots. J Int Plant Biol 59:459–47411

    Article  CAS  Google Scholar 

  • Murashige T, Skoog FA (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Narváez-Vásquez J, Ryan AC (2002) The systemin precursor gene regulates both defensive and developmental genes in Solanum tuberosum. Proc Natl Acad Sci 99:15818–15821

    Article  Google Scholar 

  • Pruski K, Astatkie T, Nowak J (2002) Jasmonate effects on in vitro tuberization and tuber bulking in two potato cultivars (Solanum tuberosum L.) under different media and photoperiod condition. In Vitro Cell Dev Biol - Plant 38:203–209

    Article  CAS  Google Scholar 

  • Puchta H, Swoboda P, Hohn B (1995) Induction of intrachromosomal homologous recombination in whole plants. Plant J 7:203–210

    Article  CAS  Google Scholar 

  • Rissel D, Heym PP, Thor K, Brandt W, Wessjohann LA, Peiter E (2017) No silver bullet-canonical poly (ADP-ribose) polymerases (PARPs) are no universal factors of abiotic and biotic stress resistance of Arabidopsis thaliana. Front Plant Sci 8:1–14

    Article  Google Scholar 

  • Rissel D, Peiter E (2019) Poly(ADP-ribose) polymerases in plant and their human counterparts: parallels and peculiarities. Int J Mol Sci 20:1–29

    Article  Google Scholar 

  • Samant A, Kumar VA, Kumar A, Dhukla PS, Joshi KK (2018) In vitro microtuber production in potato cultivar kufri himalini. Adv Plants Agric Res 8:648–653

    Google Scholar 

  • Schultz P, Jansseune K, Degenkolbe T, Meret M, Claeys H, Skirycz A, Teige M, Willmitzer L, Hannah MA (2014) Poly (ADP-ribose) polymerase controls plant growth by promoting leaf cell number. PLoS One 9:e90322

    Article  Google Scholar 

  • Schultz P, Neukermans J, Van Der Kelen K, Mühlenbock P, Van Breusegem F, Noctor G, Teige M, Metzlaff M, Matthew M, Hannah A (2012) Chemical PARP inhibition enhances growth of Arabidopsis and reduces anthocyanin accumulation and the activation of stress protective mechanisms. PLoS One 7:e37287

    Article  Google Scholar 

  • Song J, Keppler BD, Wise RR, Bent AF (2015) PARP2 is the predominant poly(ADP-ribose) polymerase in Arabidopsis DNA damage and immune responses. PLoS Genet 11:e1005200

    Article  Google Scholar 

  • Strain AJ (1985) Inhibitors of ADP-ribosyl transferase enhance the transformation of NIH3T3 cells following transfection with SV40 DNA. Exp Cell Res 159:531–535

    Article  CAS  Google Scholar 

  • Su ZZ, Zhang P, Fisher PB (1990) Enhancement of viral and DNA mediated transformation of cloned rat embryo fibroblast cells by 3-aminobenzamide. Mol Carcinog 3:309–318

    Article  CAS  Google Scholar 

  • Trujillo C, Rodriguez-Arango E, Jaramillo S, Hoyos R, Orduz S, Arango R (2001) One-step transformation of two Andean potato cultivars Solanum tuberosum L. subsp. Andigena. Plant Cell Rep 20:637–641

    Article  CAS  Google Scholar 

  • Vanderauwera S, De Block M, Van de Steene N, Van de Cotte B, Metzlaff M, Van Breusegem F (2007) Silencing of poly (ADP-ribose) polymerase in plants alters abiotic stress signal transduction. Proc Natl Acad Sci 104:15150–15155

    Article  CAS  Google Scholar 

  • Visser RGF (1991) Transformation of potato. In: Lindsey K (ed) Plant Tissue Culture Manual, vol B5. Kluwer Academic Publishers, pp 1–9

Download references

Acknowledgments

We thank Andres Narvaez for assistance in figure and manuscript preparation.

Author contribution statement

MLOC conceived and designed the research, and wrote the manuscript; DJG and VJC conducted the experimental research and helped with the preparation of tables and figures; and JNV helped with data analysis and writing and manuscript preparation.

Funding

The College of Natural and Agricultural Sciences of the University of California Riverside supported this research.

Author information

Author notes

  1. Venkateswari J. Chetty, Dora J. García, Javier Narváez-Vásquez & Martha L. Orozco-Cárdenas

    Present address: Plant Transformation Research Center, University of California Riverside, Riverside, CA, 92521, USA

  2. Venkateswari J. Chetty

    Present address: Department of Botany and Plant Sciences, University of California Riverside, Riverside, CA, 92521, USA

  3. Dora J. García

    Present address: Facultad de Ciencias Agropecuarias, Universidad de Caldas, Manizales, Colombia

Authors and Affiliations

    Authors
    1. Venkateswari J. Chetty
      View author publications

      You can also search for this author in PubMed Google Scholar

    2. Dora J. García
      View author publications

      You can also search for this author in PubMed Google Scholar

    3. Javier Narváez-Vásquez
      View author publications

      You can also search for this author in PubMed Google Scholar

    4. Martha L. Orozco-Cárdenas
      View author publications

      You can also search for this author in PubMed Google Scholar

    Corresponding author

    Correspondence to Martha L. Orozco-Cárdenas.

    Ethics declarations

    Conflict of interest

    The authors declare they have no conflict of interest and comply with ethical standards.

    Additional information

    Editor: Charles Armstrong

    Rights and permissions

    Reprints and permissions

    About this article

    Check for updates. Verify currency and authenticity via CrossMark

    Cite this article

    Chetty, V.J., García, D.J., Narváez-Vásquez, J. et al. Poly (ADP-ribose) polymerase inhibitor 3-methoxybenzamide enhances in vitro plant growth, microtuberization, and transformation efficiency of blue potato (Solanum tuberosum L. subsp. andigenum). In Vitro Cell.Dev.Biol.-Plant 56, 833–841 (2020). https://doi.org/10.1007/s11627-020-10086-9

    Download citation

    • Received:

    • Accepted:

    • Published:

    • Issue Date:

    • DOI: https://doi.org/10.1007/s11627-020-10086-9

    Keywords

    Search

    Navigation