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Adsorption of Alkaline Phosphates on Palygorskite and Sepiolite: A Tradeoff Between Enzyme Protection and Inhibition

Published online by Cambridge University Press:  01 January 2024

Mehran Shirvani ,
Banafshe Khalili Open the ORCID record for Banafshe Khalili [Opens in a new window] ,
Mahmoud Kalbasi ,
Hossein Shariatmadari  and
Farshid Nourbakhsh
Mehran Shirvani
Affiliation:
Department of Soil Science, College of Agriculture, Isfahan University of Technology, Isfahan 8415683111, Iran
Banafshe Khalili*
Affiliation:
Department of Soil Science, College of Agriculture, Isfahan University of Technology, Isfahan 8415683111, Iran
Mahmoud Kalbasi
Affiliation:
Department of Soil Science, College of Agriculture, Isfahan University of Technology, Isfahan 8415683111, Iran
Hossein Shariatmadari
Affiliation:
Department of Soil Science, College of Agriculture, Isfahan University of Technology, Isfahan 8415683111, Iran
Farshid Nourbakhsh
Affiliation:
Department of Soil Science, College of Agriculture, Isfahan University of Technology, Isfahan 8415683111, Iran
*
*E-mail address of corresponding author: bkhalili@cc.iut.ac.ir
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Abstract

Enzymes adsorbed on clay minerals and soil colloids may exhibit lower activities compared to those of free enzymes. A particular toxic metal may affect the activity of the adsorbed enzyme less critically than that of the free form, however. This information is necessary for predicting catalytic performances of clay-immobilized enzymes in natural soils as well as in food, pharmaceutical, and chemical systems. The objective of the present study was to find out how adsorption on palygorskite and sepiolite minerals modifies the catalytic activity and the Michaelis–Menten kinetics of alkaline phosphatase (ALP). Inhibition kinetics of adsorbed ALP by Cd was also compared to that of the free enzyme. The results revealed that the affinity to the substrate and the maximum reaction velocity of ALP decreased upon adsorption on the fibrous clay minerals. The ALP adsorbed maintained a reasonably high activity recovery (AR) compared to the free enzyme. The AR of the adsorbed ALP ranged from 76.9 to 92.5% for palygorskite and from 71.2 to 90.2% for sepiolite, depending on the substrate concentration applied. The presence of Cd decreased the affinity to the substrate of both the free and the adsorbed ALP, while the maximum reaction velocity remained nearly unchanged, indicating that the inhibitory effects of Cd on both the free and adsorbed ALP activities were competitive in nature. The adsorbed enzyme, however, was inhibited less severely by Cd compared to the free enzyme. The adsorption of ALP on the fibrous clay minerals, therefore, maintains the ALP activity to a great extent and provides more resistance for the enzyme against the inhibitory effects of Cd.

Keywords

Enzyme kineticsEnzyme immobilizationFibrous clay mineralsHeavy metals
Type
Article
Information
Clays and Clay Minerals , Volume 68 , Issue 4 , August 2020 , pp. 287 - 295
Copyright
Copyright © Clay Minerals Society 2020

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