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The genome insights of Streptomyces lannensis T1317-0309 reveals actinomycin D production

The Journal of Antibiotics volume 73, pages 837–844 (2020)Cite this article

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Abstract

The members of Streptomyces have been identified as a major source of antimicrobial agents with broad spectrum. This study is mainly focused on bioactivity-guided isolation and characterization of bioactive molecule from strain Streptomyces sp. T1317-0309 and its whole-genome sequence analysis for possible isolation of novel natural products. Strain Streptomyces sp. T1317-0309 showed 100% sequence similarity with strain Streptomyces lannensis TA4-8T consisting 10, 453,255 bp of genome with 5 scaffolds and 69.9 mol% G + C content. The genome analyses revealed a total of 17 putative biosynthetic gene clusters (BGCs) responsible for various secondary metabolites including actinomycin, bacteriocin, ectoine, melanin, terpene, siderophore, betalactone, NRPS, T2PKS, and T3PKS. The BGC and bioactivity-guided purification of ethyl acetate extract of strain T1317-0309 showed the great potency of antimicrobial activities against various gram-positive multi-drug resistant human pathogens including MRSA. The BGC-predicted bioactive secondary metabolite was identified by various NMR analyses and confirmed as actinomycin D. In addition, this study reveals the first genome study of Streptomyces lannensis as a novel source for actinomycin D.

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Funding

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2019R1F1A1058501).

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Author notes

  1. These authors contributed equally: Ram Hari Dahal, Tuan Manh Nguyen

Authors and Affiliations

  1. Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Kyonggi-Do, 16227, Republic of Korea

    Ram Hari Dahal, Tuan Manh Nguyen & Jaisoo Kim

  2. Thainguyen University of Agriculture and Forestry, Quyet Thang, Thai Nguyen, Vietnam

    Tuan Manh Nguyen

  3. Department of Pharmaceutical Engineering and Biotechnology, Sun Moon University, 70 Sunmoon-ro 221, Ansan-si, Chungnam, 31460, Republic of Korea

    Ramesh Prasad Pandey, Tokutaro Yamaguchi & Jae Kyung Sohng

  4. Department of Life Science and Biochemical Engineering, Sun Moon University, 70 Sunmoon-ro 221, Ansan-si, Chungnam, 31460, Republic of Korea

    Ramesh Prasad Pandey, Tokutaro Yamaguchi & Jae Kyung Sohng

  5. Department of Chemistry, College of Natural Sciences, Kyonggi University, Suwon, Kyonggi-Do, 16227, Republic of Korea

    Jongsung Noh & Seung-Woon Myung

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  1. Ram Hari Dahal
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Correspondence to Jaisoo Kim.

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Dahal, R.H., Nguyen, T.M., Pandey, R.P. et al. The genome insights of Streptomyces lannensis T1317-0309 reveals actinomycin D production. J Antibiot 73, 837–844 (2020). https://doi.org/10.1038/s41429-020-0343-0

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