Abstract
Salinity hinders agricultural productivity worldwide by distressing plant metabolism. Growth of blackgram (Vigna mungo L. var. Sulata), an adverse climate-resistant pulse, is arrested under salinity. Present research integrates study of physio-biochemical parameters and non-targeted metabolomics approach to explore the alterations in metabolic pathway during adaptive responses of nonprimed and haloprimed blackgram seedlings grown hydroponically under NaCl stress. Salinity provoked accumulation of peroxides, compatible solutes and phenolics which increased free radical scavenging activities of nonprimed seedlings under salinity. Pre-germination seed halopriming abrogated NaCl-mediated adversities in haloprimed plantlets favouring better growth. Thus, farmers may adopt seed halopriming technique to improve blackgram productivity in saline-prone fields. Additionally, metabolomics study uncovered numerous metabolites amongst which 35 compounds altered significantly under salinity. The candidate metabolites were aspartic acid, l-glutamic acid, l-proline, l-asparagine, dl-isoleucine, l-homoserine, citrulline, l-ornithine, d-altrose, d-allose, N-acetyl-d-mannosamine, fructose, tagatose, sucrose, d-glucose, maltose, glycerol-1-phosphate, d-sorbitol, benzoic acid, shikimic acid, 4-hydroxycinnamic acid, arbutin, succinic acid, pipecolic acid, fumaric acid, nicotinic acid, l-pyroglutamic acid, oxalic acid, glyceric acid, maleamic acid, adenine, guanosine, lauric acid, stearic acid and porphine. Comparing metabolic responses of nonprimed and haloprimed seedlings, it was clear that efficient alteration in carbohydrate metabolism, phenolics accumulation, amino acid, organic acid and nucleic acid metabolism were the key places of metabolic reprogramming for tolerating salinity. Overall, we report, for the first time, 35 contributory candidate compounds that constituted core fundamental metabolome invoking salinity tolerance in nonprimed and haloprimed blackgram. These metabolites may be targeted by biotechnologists to produce high vigour salt-tolerant transgenic blackgram via genetic engineering.
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This work was supported by the Department of Science and Technology, Government of West Bengal under Grant No. 1012 (Sanc.)/ST/P/S&T/2G-2013 financially and the Department of Science and Technology and Fund for Improvement of S&T Infrastructure (DST-FIST) Programme, Government of India, for instrumental facility.
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Biswas, S., Biswas, A.K. & De, B. Influence of sodium chloride on growth and metabolic reprogramming in nonprimed and haloprimed seedlings of blackgram (Vigna mungo L.). Protoplasma 257, 1559–1583 (2020). https://doi.org/10.1007/s00709-020-01532-x
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DOI: https://doi.org/10.1007/s00709-020-01532-x