Skip to main content
SpringerLink
Log in

A Simple Strategy for Methylene Blue Determination in Human and Veterinary Dosage Forms by Digital Imaging

  • ARTICLES
  • Published:
Journal of Analytical Chemistry Aims and scope Submit manuscript

Abstract

A colorimetric method based on digital image processing and an image capture device as a detector has been developed for determining methylene blue (MB) in human and veterinary dosage forms. Operational variables, such as capture devices, angle and modes of capture as well as measuring vessel geometry, were evaluated in order to establish the most suitable conditions to obtain accurate measurements. Three different capture devices including a webcam, a compact digital camera and a smartphone camera were compared. Digital images were analyzed using the open source program ImageJ. Suitable results were achieved when using a 20.7 MP back camera of a smartphone and white-LED lighting conditions. Grayscale values were finally employed as response to determine MB concentration. Satisfactory linearity was achieved in the concentration range from 3.5 × 10–7 to 4.7 × 10–6 M, with the determination coefficient R2 > 0.97 and the limit of detection of 1.7 × 10–7 M. The proposed approach was successfully applied to the detection of MB in two commercially available dosage forms for human and veterinary treatment.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.

Similar content being viewed by others

REFERENCES

  1. Rojas, J.C., Bruchey, A.K., and Gonzalez-Lima, F., Prog. Neurobiol., 2012, vol. 96, p. 32.

    Article  CAS  Google Scholar 

  2. Rumbeiha, W.K. and Oehme, F.W., Vet. Hum. Toxicol., 1992, vol. 34, no. 2, p. 120.

    CAS  PubMed  Google Scholar 

  3. Li, C., Huang, Y., Lai, K., Rasco, B.A., and Fan, Y., Food Control, 2016, vol. 65, p. 99.

    Article  CAS  Google Scholar 

  4. Thevarajah, S., Huston, T.L., and Simmons, R.M., Am. J. Surg., 2005, vol. 189, no. 2, p. 236.

    Article  Google Scholar 

  5. Schirmera, R.H., Adlera, H., Pickhardt, M., and Mandelkow, E., Neurobiol. Aging, 2011, vol. 32, no. 12, p. 2325.

    Google Scholar 

  6. Kim, S.J., Ha, D.J., and Koo, T.S., Biomed. Chromatogr., 2014, vol. 28, no. 4, p. 518.

    Article  CAS  Google Scholar 

  7. Gaudette, N.F. and Lodge, J.W., J. Anal. Toxicol., 2005, vol. 29, p. 28.

    Article  CAS  Google Scholar 

  8. Qi, J., Li, X., Zheng, H., Li, P., and Wang, H., J. Hazard. Mater., 2015, vol. 293, p. 105.

    Article  CAS  Google Scholar 

  9. Lyra, W., Bezerra dos Santos, V., Gama Dionízio, A.G., Lacerda Martins, V., Farias Almeida, L., Nóbrega Gaião, E., Gonçalves Dias Diniz, P.H., Silva, E.C., and Araújo, M.C.U., Talanta, 2009, vol. 77, no. 5, p. 1584.

    Article  Google Scholar 

  10. Lima, M.B., Andrade, S.L.E., Barreto, I.S., Almeida, L.F., and Araújo. M.C.U., Microchem. J., 2013, vol. 106, p. 238.

    Article  CAS  Google Scholar 

  11. Koenig, M.H., Yi, E.P., Sandridge, M.J., Mathew, A.S., and Demas, J.N., J. Chem. Educ., 2015, vol. 92, no. 2, p. 310.

    Article  CAS  Google Scholar 

  12. Young, T., in Sources of Color Science, MacAdam, D.L., Ed., Cambridge: MIT Press, 1970, p. 51.

    Google Scholar 

  13. von Helmholtz, H., Treatise on Physiological Optics, Southall, J.P.C., Ed., New York: Dover, 1962.

    Google Scholar 

  14. Apyari, V.V., Gorbunova, M.V., Isachenko, A.I., Dmitrienko, S.G., and Zolotov, Yu.A., J. Anal. Chem., 2017, vol. 72, no. 11, p. 1127.

    Article  CAS  Google Scholar 

  15. Wang, Y., Li, Y., Bao, X., Han, J., Xia, J., Tian, X., and Ni, L., Talanta, 2016, vol. 160, p. 194.

    Article  CAS  Google Scholar 

  16. Pessoa, K.D., Suarez, W.T., Dos Reis, M.F., de Oliveira Krambeck Franco, M., Moreira, R.P.L., and dos Santos, V.B., Spectrochim. Acta, Part A, 2017, vol. 185, p. 310.

    Article  CAS  Google Scholar 

  17. Apyari, V.V. and Dmitrienko, S.G., J. Anal. Chem., 2008, vol. 63, no. 6, p. 530.

    Article  CAS  Google Scholar 

  18. Farmacopea Argentina: Azul de Metileno, Buenos Aires: Natl. Administration of Drugs, Foods and Medical Devices (ANMAT), 2003, 7th ed., vol. 2.

  19. ImageJ: Image Processing and Analysis in Java. https://imagej.nih.gov/ij/. Accessed January 1, 2020.

  20. Clark, J.J. and Skaff, S., J. Opt. Soc. Am. A, 2009, vol. 26, no. 11, p. 2488.

    Article  Google Scholar 

  21. Lima, M.B., Andrade, S.I.E., Silva Neta, M.S., Barreto, I.S., Almeida, L.F., and Araújo, M.C.U., J. Braz. Chem. Soc., 2014, vol. 25, no. 5, p. 898.

    CAS  Google Scholar 

  22. Currie, L.A., Pure Appl. Chem., 1995, vol. 67, no. 10, p. 1699.

    Article  CAS  Google Scholar 

  23. United States Pharmacopoeia XX, Rockville, MD: US Convention, 1980, p. 519.

  24. Khan, M.R., Khan, M.A., Alothman, Z.A., Alsohaimi, I.H., Naushada, M., and Al-Shaalan, N.H., RSC Adv., 2014, vol. 4, p. 34037.

    Article  CAS  Google Scholar 

Download references

Funding

F. Ávila and V. Springer would like to express their gratitude to National Council of Scientific and Technical Research (CONICET) for their post-doctoral fellowships. The authors acknowledge the financial support from SGCyT – Universidad Nacional del Sur, PLAPIQUI and INQUISUR-CONICET (Argentina).

Author information

Authors and Affiliations

  1. INQUISUR, Departamento de Química, Universidad Nacional del Sur (UNS)-CONICET, 8000, Bahía Blanca, Argentina

    Valeria Springer, Francisco Avila & Marcelo Avena

Authors
  1. Valeria Springer
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Francisco Avila
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Marcelo Avena
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Valeria Springer.

Ethics declarations

The authors declare that there is no conflict of interests regarding the publication of this paper.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Valeria Springer, Avila, F. & Avena, M. A Simple Strategy for Methylene Blue Determination in Human and Veterinary Dosage Forms by Digital Imaging. J Anal Chem 75, 958–964 (2020). https://doi.org/10.1134/S1061934820070151

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1061934820070151

Keywords:

Search

Navigation