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OsGSTU6 Contributes to Cadmium Stress Tolerance in Rice by Involving in Intracellular ROS Homeostasis

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Abstract

Glutathione S-transferases (GSTs) are multifunctional proteins that play important roles in cellular detoxification as well as in plant growth and development. Here, we cloned a new rice tau class GST gene, OsGSTU6, and evaluated its roles in regulating cadmium (Cd) stress tolerance of rice. OsGSTU6 is located both in the cytosol and nucleus of cells. The transcripts of OsGSTU6 were detected in the whole life cycle of rice with higher levels in the leaf blade and sheath at heading stage. The transcripts of OsGSTU6 were significantly stimulated by a number of environmental factors including several phytohormones, abiotic stresses, and heavy metals, suggesting its stress-responsive characteristics. Moreover, the overexpression (OE) of OsGSTU6 in rice reduced the accumulation of Cd in leaf and enhanced the tolerance of the plant to Cd stress, while knockdown (RNAi) of OsGSTU6 triggered Cd accumulation in rice leaves and decreased stress tolerance. Meanwhile, the expression levels of several candidate genes responsible for scavenging reactive oxygen species (ROS) were downregulated in OE of OsGSTU6 lines. In addition, the content of superoxide anions (O2−) and glutathione showed reduction in OE plants, but was augmented in RNAi plants under both normal growth and Cd stress, compared with wild type. Furthermore, both the yeast two-hybrid assay and firefly luciferase complementation imaging (LCI) assay demonstrated that OsGSTU6 might function with dimers in plant cells. All these results suggest that OsGSTU6 may play an important role in Cd stress tolerance of rice by involving in the intracellular ROS homeostasis.

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Acknowledgements

This work was supported by the Shaanxi Provincial Key Project of Research and Development Plan, China (2019SF-249) and the National Natural Science Foundation of China (Grant Nos. 31770204 and 31270299). The funders had no role in the designing and performing the experiments.

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  1. Xiu-Qing Jing and Meng-Ru Zhou have contributed equally to this work

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  1. State Key Laboratory of Crop Stress Biology in Arid Area/College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, The People’s Republic of China

    Xiu-Qing Jing, Meng-Ru Zhou, Xiu-Min Nie, Lu Zhang, Peng-Tao Shi, Abdullah Shalmani, Hai Miao, Wen-Qiang Li, Wen-Ting Liu & Kun-Ming Chen

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X-QJ and M-RZ have contributed equally to this work. K-MC, X-QJ, and M-RZ conceived and designed the research. X-QJ and M-RZ performed the experiments, analyzed the data, and wrote the article. HM cloned the sequence and constructed the transgenic lines. P-TS and AS cultivated rice plants and extracted RNA. X-MN and LZ constructed vectors. K-MC and W-TL improved and commented on the article. K-MC supported the research. All authors read the article and approved the final version.

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344_2020_10148_MOESM1_ESM.tif

Survival rates statistics of OsGSTU6-transgenic plants treated with 1 mM Cd for 14 days. Data are means ±SD from three independent biological replicates (We planted seeds on April 2, August 20 and November 5, 2018, respectively, and then carried out the experiments and obtained results, as shown in A, B and C). Supplementary file1 (TIF 10743 kb)

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Jing, XQ., Zhou, MR., Nie, XM. et al. OsGSTU6 Contributes to Cadmium Stress Tolerance in Rice by Involving in Intracellular ROS Homeostasis. J Plant Growth Regul 40, 945–961 (2021). https://doi.org/10.1007/s00344-020-10148-7

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