Abstract
Key Message A resistant E. grandis genotype showed a constitutive overexpression of genes related to resistance to myrtle rust caused by A. psidii .
Abstract Myrtle rust caused by Austropuccinia psidii is considered one of the most important fungal diseases affecting Eucalyptus spp. plantations in Brazil. Although the selection and planting of resistant eucalypt genotypes have been the major strategies to manage the disease in Brazil, the molecular mechanisms involved in resistance are still unclear. In this study, we evaluated the gene expression profile of two contrasting Eucalyptus grandis genotypes in resistance level to rust by RNA-Seq. The two genotypes showed a very different background gene expression level even without A. psidii infection. The resistant genotype had a constitutive overexpression of a large number of protein-coding genes compared to the susceptible genotype. These genes were mainly associated with signal transduction, photosynthesis, regulation and response to salicylic acid (SA), and protein kinase leucine-rich receptors (PK-LRR). PK-LRR and SA mediated disease resistance are well known to be effective against obligate biotroph pathogens, such as A. psidii. In addition, at 24 h after infection, the susceptible genotype was able to activate some response, however, several resistance-related proteins had their expression level reduced with A. psidii infection. Here, we present the first analysis of E. grandis genotypes transcriptomes infected by A. psidii and it reveals a constitutive overexpression of several resistance-related genes in the resistant genotype compared to the susceptible one. Our findings have the potential to be used as candidate molecular markers for resistance to myrtle rust.
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Data availability
The sequencing raw data from 12 RNA-Seq libraries were deposited on the Sequence Read Archive from NCBI under SRA accession: PRJNA588626. Data are available here: https://www.ncbi.nlm.nih.gov/sra/PRJNA588626 and will be made publicly accessible after the publication of the manuscript. Additionally, the processed data including all supplementary files are temporally available here: https://figshare.com/s/8caab8398ff2002df311; and will be publish under the following https://doi.org/10.6084/m9.figshare.12061626.
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Acknowledgements
The authors thank to Clonar Resistência a Doenças Florestais for providing the plant material and the inoculation facilities. We also thanks to Plant and Food Research for providing all bioinformatics facilities and Núcleo de Análise de Biomoléculas (NuBioMol) for supporting data analysis. NuBioMol is supported by Fundação de Amparo à Pesquisa do Estado de Minas Gerais (Fapemig), CAPES, CNPq, Financiadora de Estudos e Projetos (Finep) and Sistema Nacional de Laboratórios em Nanotecnologias (SisNANO)/Ministério da Ciência, Tecnologia e Informação (MCTI).
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This work was supported by CNPq (Conselho Nacional de Desenvolvimento Cientıfico e Tecnologico, Grant No. 201179/2018-0), CAPES (Coordenação de Aperfeicoamento de Pessoal de Nivel Superior), and Suzano S.A.
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All the authors conceived and designed the experiments; SAS conducted the experiments; SAS and PMPV performed the data analysis; SAS wrote the manuscript text; all the authors revised and contributed to the final version of the manuscript; MDT and ACA supervised the research.
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Santos, S.A., Vidigal, P.M.P., Guimarães, L.M.S. et al. Transcriptome analysis of Eucalyptus grandis genotypes reveals constitutive overexpression of genes related to rust (Austropuccinia psidii) resistance. Plant Mol Biol 104, 339–357 (2020). https://doi.org/10.1007/s11103-020-01030-x
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DOI: https://doi.org/10.1007/s11103-020-01030-x