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Interference with miR-210 Alleviated Renal Injury in Septic Rats by Inhibiting JAK-STAT Pathway

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Abstract

Pediatric sepsis has become the leading cause of death in pediatric intensive care units (PICU). The regulation of target genes may be the key to the treatment of pediatric sepsis. The expression of miR-210 in rat serum was detected by RT-qPCR. The serum BUN, Scr, and CysC were detected by an automatic biochemical analyzer. The expression of inflammatory factors was detected by ELISA. The apoptosis level of the cells was detected by TUNEL staining. The expression of apoptotic proteins Bcl2, Bax, Cleaved caspase3, caspase3, and JAK/STAT3 pathway-related proteins were detected by western blot. The expression of miR-210 was abnormally elevated in sepsis pups. Interfering with the expression of miR-210 in rats could reduce the degree of renal injury and inhibit the inflammatory response in sepsis pups. In addition, interference with miR-210 could inhibit the apoptosis level of renal tissue cells, and the expression of apoptosis-related proteins was also significantly decreased. During this process, we found that after interfering with the expression of miR-210, the expression of the JAK/STAT pathway was inhibited. Then, pathway agonist SC-39100 can reverse the inhibitory effects of interfering with miR-210 on renal tissue damage, inflammatory response, and apoptosis. Interference with miR-210 alleviated renal injury in septic rats by inhibiting JAK-STAT pathway.

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Authors and Affiliations

  1. Department of Critical Care Medicine, The People’s Hospital of Longhua, Shenzhen, Shenzhen, 518109, Guangdong Province, China

    Benchao Zhang, Ziye Guo & Siqi Lai

  2. Department of Infection Disease, Children’s Hospital of Nanjing Medical University, No.72, Guangzhou road, Gulou District, Nanjing, Jiangsu Province, 210000, China

    Hongjian Chen

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  1. Benchao Zhang
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  2. Ziye Guo
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  3. Siqi Lai
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  4. Hongjian Chen
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All authors read and approved the final version of the manuscript.

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Correspondence to Hongjian Chen.

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The authors declare that they have no competing interests.

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The study protocol was approved by the People’s Hospital of Longhua, Shenzhen. All animal experiments comply with the ethical requirements of the animal council.

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Zhang, B., Guo, Z., Lai, S. et al. Interference with miR-210 Alleviated Renal Injury in Septic Rats by Inhibiting JAK-STAT Pathway. Inflammation 43, 2156–2165 (2020). https://doi.org/10.1007/s10753-020-01283-0

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