Abstract
We studied the effect of acrylamide on the content of intracellular ATP in the cells of bacteria of the genera Rhodococcus and Alcaligenes, the luminescence of the genetically engineered strain Escherichia coli K12 TG1 (pXen7), and the survival of bacteria of various systematic groups. According to the level of decrease in the concentration of intracellular ATP, it was found that the strain with lower amidase activity (R. erythropolis 6-21) and Gram-negative proteobacteria A. faecalis 2 were the most sensitive to acrylamide after a 20-min exposure, while the strain R. ruber gt 1 was stable, having a high nitrile hydratase activity in combination with a low amidase activity. EC50 of acrylamide for 2 h was 7.1 g/L for E. coli K12 TG1 (pXen7). Acrylamide at a concentration of 10–20 mM added to the culture medium led to a slight decrease in the number of CFUs of Rhodococcus, A. faecalis 2, and E. coli compared to the control. At an acrylamide concentration of 250 mM, from 0.016 to 0.116% of viable bacterial cells remained, and a solution of 500 mM and higher inhibited the growth of the majority of the studied strains. The results confirm that acrylamide is much less toxic to prokaryotes than to eukaryotes.
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ACKNOWLEDGMENTS
We are grateful to I.L. Maslennikova, Senior Research Associate (Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Center, Ural Branch, Russian Academy of Sciences), for kindly providing the E. coli strain K12 TG1 (pXen7) for this study.
Funding
This work was carried out under the state assignment (topic “Molecular Mechanisms of Adaptation of Microorganisms to Environmental Factors,” registration no. R&D AAAA-A19-119112290009-1).
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Translated by M. Batrukova
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Maksimova, Y.G., Mochalova, E.M. & Demakov, V.A. Influence of Acrylamide on Energy Status and Survival of Bacteria of Different Systematic Groups. Dokl Biochem Biophys 492, 117–120 (2020). https://doi.org/10.1134/S1607672920030035
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DOI: https://doi.org/10.1134/S1607672920030035