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Epitope Analysis of Murine and Chimeric Monoclonal Antibodies Recognizing the Cancer Testis Antigen PRAME

  • BIOCHEMISTRY, BIOPHYSICS, AND MOLECULAR BIOLOGY
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Abstract

We investigated the epitope specificity of different monoclonal antibodies recognizing the cancer testis antigen PRAME. Antibody 5D3 binds to the fragment of PRAME corresponding to 160–180 amino acid residues. Antibodies 6H8 and F11 bind to the fragment corresponding to 180–200 amino acid residues of PRAME. These antibodies retained the ability to recognize these PRAME fragments after chimerization.

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Funding

This study was supported by the Ministry of Science and Higher Education of the Russian Federation (agreement no. 075-15-2019-1249 of June 10, 2019; unique project identifier no. RFMEFI60418X0204).

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Authors and Affiliations

  1. Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation, Moscow, Russia

    V. A. Misyurin, Yu. P. Finashutina, M. V. Larina, O. N. Solopova, N. A. Lyzhko, L. A. Kesaeva, N. N. Kasatkina & T. K. Aliev

  2. OOO Genotechnologiya, Moscow, Russia

    A. A. Turba & A. V. Misyurin

  3. Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia

    T. K. Aliev &  M. P. Kirpichnikov

  4. Moscow State University, Moscow, Russia

    M. P. Kirpichnikov

Authors
  1. V. A. Misyurin
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  2. Yu. P. Finashutina
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  3. A. A. Turba
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  4. M. V. Larina
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  5. O. N. Solopova
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  6. N. A. Lyzhko
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  7. L. A. Kesaeva
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  8. N. N. Kasatkina
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  9. T. K. Aliev
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  10. A. V. Misyurin
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  11. M. P. Kirpichnikov
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Corresponding author

Correspondence to V. A. Misyurin.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by M. Batrukova

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Misyurin, V.A., Finashutina, Y.P., Turba, A.A. et al. Epitope Analysis of Murine and Chimeric Monoclonal Antibodies Recognizing the Cancer Testis Antigen PRAME. Dokl Biochem Biophys 492, 135–138 (2020). https://doi.org/10.1134/S1607672920030072

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  • DOI: https://doi.org/10.1134/S1607672920030072

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