Abstract
The allophycocyanin (APC) protein purified from Phormidium sp. A09DM was investigated for its in vivo antioxidant and anti-aging potential in Caenorhabditis elegans. An increased mean lifespan of APC-treated (100 μg/ml) worms (wild type) were observed from 16 ± 0.2 days (control) to 20 ± 0.1 days (treated). APC-treated worms also showed improved physiological marker of aging such as the rate of pharyngeal pumping and higher rate of survival against oxidative and thermal stress. Furthermore, APC was found to moderate the expression of human amyloid beta (Aβ1–42) as well as associated Aβ-induced paralysis in the transgenic C. elegans CL4176 upon increase in temperature. Furthermore, RNA interference (RNAi)-mediated studies revealed the dependence of downstream regulator daf-16, independent of stress-induced resistance gene skn-1 in the APC treated C. elegans. In the present study, we tried to demonstrate the anti-aging activity, longevity and protective effects of APC against cellular stress in C. elegans, which can lead to the use of this biomolecule in drug development for age-related disorders.
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Abbreviations
- C. elegans :
-
Caenorhabditis elegans
- PE:
-
Phycoerythrin
- PC:
-
Phycocyanin
- NGM:
-
Nematode growth medium agar plate
- CGC:
-
Caenorhabditis Genetic Center
- ROS:
-
Reactive oxygen species
- Aβ:
-
Amyloid-beta
- PBP:
-
Phycobiliprotein
- SDS:
-
Sodium dodecyl sulfate
- PAGE:
-
Polyacrylamide gel electrophoresis
- L4:
-
Larval stage 4
- H2O2 :
-
Hydrogen peroxide
- DCFH-DA:
-
Dichloro-dihydro-fluorescein-diacetate
- PQ:
-
Paraquat
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Acknowledgements
NKS acknowledges Science and Engineering Research Board (SERB), Department of Science and Technology (DST), Ministry of Science and Technology, Government of India, for Young Scientist fellowship under the scheme of startup research grant (SB/YS/LS-290/2013). MGC also acknowledges SERB for Senior Research Fellowship under the scheme of startup research grant (SB/YS/LS-290/2013).
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The research was supported by the Department of Science and Technology (DST), Ministry of Science and Technology, Government of India under the scheme of startup research grant (SB/YS/LS-290/2013)
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Conceptualization: MGC, SNP; DM, RPR, NKS. Methodology: MGC, SNP; DM, NKS, RPR. Formal analysis and investigation: MGC, SNP; DM, NKS, RPR. Writing—original draft preparation: MGC. Writing—review and editing: DM, NKS, RPR. Resources: Shri. A. N. Patel P. G. Institute of Science and Research, Anand, Post Graduate Department of Bioscience, Sardar Patel University, Anand. Supervision: NKS, RPR.
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Mukesh Ghanshyam Chaubey, Stuti Nareshkumar Patel, Rajesh Prasad Rastogi, Datta Madamwar and Niraj kumar Singh declare that they have no conflict of interest.
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Chaubey, M.G., Patel, S.N., Rastogi, R.P. et al. Cyanobacterial pigment protein allophycocyanin exhibits longevity and reduces Aβ-mediated paralysis in C. elegans: complicity of FOXO and NRF2 ortholog DAF-16 and SKN-1. 3 Biotech 10, 332 (2020). https://doi.org/10.1007/s13205-020-02314-1
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DOI: https://doi.org/10.1007/s13205-020-02314-1