Elsevier

Food Chemistry

Volume 333, 15 December 2020, 127465
Food Chemistry

Polysaccharide/gelatin blend films as carriers of ascorbyl palmitate – A comparative study

https://doi.org/10.1016/j.foodchem.2020.127465Get rights and content

Highlights

  • Ascorbyl palmitate (AP) increases H2O content, while reduces film pH and solubility.

  • AP increases yellowness, opacity and UV-barrier capacity of the films.

  • AP improves the barrier properties and durability of gum Arabic (GAR)/gelatin film.

  • GAR offers controlled release of AP, thus prolonged antioxidant activity.

  • Octenyl succinic anhydride modified starch (OSA) offers fast release of AP.

Abstract

The comparative study was performed in order to assess the most suitable matrix polymer for ascorbyl palmitate (AP). The antioxidant (1 and 2% w/w) was loaded into the 75/25 blend films based on polysaccharides (gum Arabic (GAR), octenyl succinic anhydride modified starch (OSA), water soluble soy polysaccharides (WSSP)) and gelatin (GEL). The AP was present in the films both in the form of longitudinal crystals and microglobules. Because of amphiphilic character, the AP had the moisturizing action on the films; however, its addition reduced film solubility in water. AP did not affect the water vapor permeability and tensile strength of the OSA-based carrier. The Makoid-Banakar with Tlag model was suitable for the efficient simulation of AP release from the films. The OSA-based system offered the fastest release of AP and, consequently, had the best initial antiradical activity. The 1%AP-added GAR75/GEL25 film provided the most extended release of antioxidant capacity.

Introduction

In recent years, the concept of controlled release has attracted growing interest in terms of its use in the production of emitters – a group of modern active packaging whose task is to extend the shelf life or to preserve and improve the quality of food. Since many food products contain oxylabile compounds, the active antioxidant materials are one of the most important innovations of packaging technologies. The design of carrier material should enable releasing the active substance continuously and uniformly throughout the entire storage period, maintaining an effective concentration of the active ingredient on the surface of the packaged food, where the spoilage reactions run most intensively. The effectiveness of active packaging depends on the ability of an active substance to penetrate to or to stay in the reaction site. Both polar and non-polar antioxidants have predispositions to accumulate at the oil/air or O/W interfaces. These interfaces are in fact the sites where contact between the substrate and oxygen is facilitated. According to the theory of the so-called polar paradox, hydrophilic antioxidants are more effective in the less polar phase (e.g. oil, lard), whereas lipophilic compounds exhibit higher activity in the more polar phase (e.g. O/W emulsions, liposomes, biological membranes, tissues) (Laguerre et al., 2015). Furthermore, it can be assumed that in water-containing products, the polar antioxidants incorporated in films/coating may diffuse too readily into the aqueous phase, which in turn may lead to a decline in their effective concentration at the oil/air interface. Non-polar active substances could improve the water vapor barrier properties of packaging materials. Therefore, non-polar antioxidants can be a potentially more beneficial component of active packaging systems, than the polar ones.

Ascorbyl palmitate (AP) is a fatty acid ester of ascorbic acid (AA) often used as a strong antioxidant in fat-containing foods, e.g. flavoured fermented milk products, dehydrated milk, creams, cheese products, fats and oils, spreads and emulsions, nut butters and nut spreads (EFSA, 2015a). AP can be used as additive (E304(i)) mostly according to quantum satis except for foods for infants and young children. It is generally recognized as safe for human consumption and can be used legally as a human dietary supplement (CFR, 2020). According to the European Food Safety Authority (EFSA) Panel the available toxicological data are too limited to establish an ADI for AP, but there is no safety concern for its use at the reported uses and use levels (exposure estimates based on the high percentile for the maximum level exposure scenario range from 0.4 to 10.8 mg/kg body weight (bw)/day across all population groups). The presence of AP in oral supplements contributes to the AA content and helps protect fat-soluble antioxidants in the supplement. In the human digestive tract, the AP is fully hydrolyzed in the hepatic portal plasma and/or liver to AA and its respective fatty acid (EFSA, 2015a). Compared to water-soluble AA, AP is more stable (the esterification reduces the hydrolysis of AA) (Austria, Semenzato, & Bettero, 1997) and may be less easily lost in the urine. It could be assumed, the use of AP as a component of edible packaging would not only improve food quality but also the vitamin C nutriture of consumers (Johnston et al., 1994). Recently, AP has attracted extensive interest as an anticancer compound because of its lipophilic nature (Zhou et al., 2017).

Because of hydrophobic character of AP, the obtaining of stable emulsion is extremely difficult and requires addition of emulsifiers (e.g. Tween) (López-Martínez & Rocha-Uribe, 2017) and/or ethanol as a cosolvent (Han, Hwang, Min, & Krochta, 2008). Gelatin (GEL) is a surface-active protein that is capable of acting as an emulsifier. Moreover, because of excellent gelling properties, GEL can be used to prepare emulsions that are physically stable (Kowalczyk & Baraniak, 2014). Nevertheless, when used on its own GEL often produces relatively large droplet sizes during homogenization, so it has to be used in conjunction with other surfactants to improve its effectiveness as an emulsifier (Totre, Ickowicz, & Domb, 2011). The certain types of polysaccharides, such as gum Arabic (GAR), starch modified with octenyl succinic anhydride (OSA), and water soluble soy polysaccharides (WSSP) are commonly used as effective emulsifiers. A recent study by Łupina et al. (2019) suggests that the edible films based on the blends of GEL and the above mentioned polysaccharides could be used as effective emulsifying polymer network systems and carriers for hydrophobic biologically active substances. Combination of GEL with other polymers affects the release of active compounds. A high concentration of GEL in the carrier can significantly limit the diffusion mobility of the polymer-embedded substance (Kanth et al., 2017, Kowalczyk et al., 2020), which is not desirable since oxidation reactions on the food surface may start if the release of the antioxidant from the packaging film is too slow. Therefore, the aim of the present study was to assess the functional properties of edible 75/25 blend films based on polysaccharides (GAR, OSA, WSSP) and GEL incorporated with increasing AP contents (1% and 2%). Microstructure, water affinity, optical and mechanical properties, as well as AP release profiles and antioxidant activities of the films were investigated and compared.

Section snippets

Materials

Pork GEL (bloom strength of 240; McCormick-Kamis, Poland), GAR Agri-Spray Acacia R (Agrigum International, United Kingdom), starch sodium octenylsuccinate PurityGum®2000 (Ingredion, Germany), and WSSP (Gushen Biological Technology Group Co., China) were used in this study. AP, glycerol, Tween 80, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) were purchased from Sigma Chemical (USA), while ethanol (99.8%) and methanol (99.8%) were purchased from POCH (Poland).

Film preparation

Films were obtained from

Microstructure

Microscopic imaging showed that the GAR- and OSA-based FFSs were relatively homogenous (Fig. 1), indicating good solubility of the polymers in the water–ethanol mixture. In turn, the FFSs containing WSSP showed the grainy structure, which reveals the limited solubility of the WSSP and confirms the globular nature of soy polysaccharides in solution (Wang, Huang, Nakamura, Burchard, & Hallett, 2005). The AP was present in the emulsions both in the form of longitudinal crystals and microglobules.

Conclusion

The AP, as an ester formed from AA, slightly decreased the pH of the films. Although, in general, the AP did not improve surface hydrophobicity of most films, its presence reduced the water solubility. The amphiphilic character of AP increased the MC of the films. The WVP and TS of the OSA-based carrier were not affected by AP. In the case of the GAR-based system, the fortification with AP had beneficial effects by improving water vapor barrier properties and TS. The increase in AP

CRediT authorship contribution statement

Katarzyna Łupina: Conceptualization, Methodology, Data curation, Formal analysis, Visualization, Investigation, Writing - original draft. Dariusz Kowalczyk: Conceptualization, Methodology, Supervision, Validation, Writing - original draft. Emilia Drozłowska: Investigation, Visualization, Writing - review & editing.

Declaration of Competing Interest

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Acknowledgements

This work was financially supported by the National Science Centre (Poland) under grant number 2019/35/N/NZ9/01795.

References (40)

  • K. Łupina et al.

    Food Hydrocolloids Edible films made from blends of gelatin and polysaccharide-based emulsifiers - A comparative study

    Food Hydrocolloids

    (2019)
  • A. Nor Amalini et al.

    Exploring the properties of modified fish gelatin films incorporated with different fatty acid sucrose esters

    Food Packaging and Shelf Life

    (2018)
  • S. Palma et al.

    Evaluation of the surfactant properties of ascorbyl palmitate sodium salt

    European Journal of Pharmaceutical Sciences

    (2002)
  • S. Tesch et al.

    Stabilization of emulsions by OSA starches

    Journal of Food Engineering

    (2002)
  • Q. Wang et al.

    Molecular characterisation of soybean polysaccharides: An approach by size exclusion chromatography, dynamic and static light scattering methods

    Carbohydrate Research

    (2005)
  • S. Wang et al.

    Rheological properties and chain conformation of soy hull water-soluble polysaccharide fractions obtained by gradient alcohol precipitation

    Food Hydrocolloids

    (2019)
  • Bruschi, M. (2015). Mathematical models of drug release. In M. Bruschi (Eds.) Strategies to Modify the Drug Release...
  • Code of Federal Regulations. (2020). Food and Drug Administration, Title 21, Par....
  • S. Dash et al.

    Kinetic modeling on drug release from controlled drug delivery systems

    Acta Poloniae Pharmaceutica - Drug Research

    (2010)
  • EFSA

    Re-evaluation of polyoxyethylene sorbitan monolaurate (E432), polyoxyethylene sorbitan monooleate (E433), polyoxyethylene sorbitan monopalmitate (E434), polyoxyethylene sorbitan monostearate (E435)andpolyoxyethylene sorbitan tristearate (E436) as food additives

    EFSA Journal

    (2015)
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